Neurological disorders & 3D Histology

Microglia marked by IBA-1 (magenta) cluster around β-amyloid plaques (cyan) in brain of 7-month-old homozygous ARTE10 (disease model #16347) female mouse from Taconic Biosciences. SmartSPIM 15X objective.

Neuroscientists studying major neurological disorders such as Alzheimer’s, Parkinson’s, and Huntington’s diseases frequently use transgenic mouse models to evaluate disease progression and the impact of potential therapies. For instance, mice with overexpression of Alzheimer’s related proteins (e.g. amyloid precursor protein, APP) or mutations that trigger pathology (see Jankowsky and Zheng 2017) demonstrate the hallmarks of… Continue reading Neurological disorders & 3D Histology

LifeCanvas Portraits: Dr. Li Ye

Photo by Don Boomer

Updated April 13, 2022: Dr. Ye’s lab recently published “HYBRiD: hydrogel-reinforced DISCO for clearing mammalian bodies.” Learn more about the Ye Lab at the Scripps Research Institute. LifeCanvas (LC): How did you arrive at your current neuroscience research interests? Li Ye (LY): I have a background in peripheral metabolism, so I was interested in how… Continue reading LifeCanvas Portraits: Dr. Li Ye

Organoids in 3D: Understanding human brain development & disease

Emerging organoid technologies have been used to model human organ development and diseases “in a dish”. Organoids are derived either from induced pluripotent or organ-restricted adult stem cells. These cells are self-renewing, self-organizing and able to grow into 3D structures resembling the architecture and physiology of the original biological specimens.

How 3D tissue clearing benefits COVID-19 research

Over 190 million people have now been infected with the novel coronavirus SARS-CoV-2 in the ongoing coronavirus disease (COVID-19) pandemic with a total number of deaths passing 4.1 million worldwide. Multiple organs are affected by COVID-19, however, the lungs are the main site of disease.

Alzheimer’s biomarkers in 3D: A new perspective on an aging disease

Immunolabeled GFAP (cyan) and Beta-amyloid plaques (magenta) in a 5xFAD mouse brain model of Alzheimer’s disease. Scale bar = 100 µm, 15X objective. Sample courtesy of Epp Lab, University of Calgary.

In 1906 at the University of Munich the neuropathologist Dr. Alois Alzheimer (Figure 1) was studying the brain of a recently deceased patient, Auguste Deter. Previously Alzheimer had assisted Frau Deter in the city asylum in Frankfurt and observed that she suffered from memory loss, delusions and dementia. Working in Dr. Emil Kraepelin’s lab in Munich… Continue reading Alzheimer’s biomarkers in 3D: A new perspective on an aging disease

Whole brain molecular labeling & imaging techniques shed new light on Alzheimer’s Disease

Prof. Li-Huei Tsai’s lab at MIT is focused on uncovering the cellular and circuit-level mechanisms that underlie Alzheimer’s disease, the devastating neurological disorder that degrades the brain’s learning and memory systems. In a 2016 paper in Nature (Iaccarino et al.), the lab reported the surprising discovery that by simply entraining the activity of cortical networks… Continue reading Whole brain molecular labeling & imaging techniques shed new light on Alzheimer’s Disease