Light Sheet Microscopy

DALISPIM

DALISPIM is an open-top, inverted light sheet microscope that combines high-speed, high-resolution volumetric imaging with broad sample compatibility. Key highlights and features include:

  • Patented axial sweeping technology, which generates homogenous PSFz across field of view
  • Aberration-free optical coupling with standard sample holders (e.g. well plates, slide glasses, petri dishes, organ-on-a-chip devices) without the need for immersion baths
  • Capacity for well plates up to 125 x 85 mm, or custom containers up to 250 x 75 –  image live or cleared tissues including intact organs, organoids, biopsies, sections, and even entire model organisms
  • Custom-designed optics and patented axial sweeping technology for rapid acquisition of images with uniformly high resolution in all axes
  • Easy-to-use setup and acquisition software
  • Comprehensive post-processing package
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SmartSPIM​

The SmartSPIM light sheet microscope provides unparalleled resolution and acquisition speed throughout intact tissue volumes, while its modular design allows you to upgrade and customize individual components.
Key highlights and features include:

  • Patented axial sweeping technology, which generates homogenous PSFz across field of view
  • Rapid volumetric acquisition (intact mouse brain hemisphere in <30 minutes)
  • Large imaging chamber and dual illumination, optimized for large (up to 40mm x 75mm) or multi-sample acquisition – easily image organoids, rat brains, entire juvenile mice, & everything in between
  • Focus compensation and tunable imaging parameters
  • Intuitive acquisition software with real-time tile correction
  • Objectives available for 1.63X through 22X magnification
Learn more about SmartSPIM
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MegaSPIM Light Sheet Microscope

MegaSPIM

The MegaSPIM microscope is our newest light sheet imaging system. Utilizing the same powerful axial sweeping technology as SmartSPIM, MegaSPIM is optimized for imaging not only laterally large samples, but thin slices and arrays of small tissues as well. Unique highlights and features include:

  • Capacity for lateral sample sizes up to 200 mm x 200 mm –  easily image expanded tissues, large slabs, and arrays of organoids, slices, or whole rodent organs
  • Custom mounting solutions for a variety of sample types
  • High-throughput multicolor acquisition with dual cameras and independent focus correction
  • Post-processing tools to deskew and stitch your data into complete ready-to-analyze volumes
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Recent publications citing LifeCanvas light sheet microscopy

A human cardiomyocyte screen identifies optimized lipid nanoparticles for in vivo cardiac gene editing

Yehui Sun, Yu-Chung Pien, Yufen Xiao, Wei Tan, Efrain Sanchez-Ortiz, Mateusz Z. Durbacz, Zexiang Chen, John R. McAnally, Yu Zhang, Hui Li, Andreas C. Chai, Francesco Chemello, Fangyu Zhang, Sang M. Lee, Priyanka Patel, Denise M. Ramirez, Sumanta Chatterjee, Ning Liu, Eric N. Olson, Daniel J. Siegwart

Journal:
PNAS
Publication Date:
Research Area:
Cardiovascular, Biodistribution
Tissue Species:
Mouse
Tissue Type:
Heart
Products Cited:
SHIELD, EasyIndex, Passive Clearing Kit, SmartSPIM
The midbrain reticular formation in contextual control of perceptual decisions

Jordan R. Shaker, Jeremy N. Schroeter, Daniel Birman, Nicholas A. Steinmetz

Journal:
Neuron
Publication Date:
Research Area:
Neural Circuits
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, Passive Clearing Kit, SmartSPIM
Fronto-insular circuit mechanisms of accelerated intermittent theta burst stimulation

Shane B. Johnson, Devin Rocks, Laura Chalençon, Kenneth Johnson, Umair Hassan, Anisul Arefin, Gülcan Akgül, Alexander Donatelle, Henry Asher, Immanuel Elbau, David Estrin, Rebecca Zhang, Alexandra Lenz, Rachel Mikofsky, Cory Knox, Rachael Han, Pooja Suganthan, Tahrima Chowdhury, Christine Kuang, Daniel Shaver, Parsa Nilchian, Puja Parekh, Jacob Roshgadol, Natalia DeMarco Garcia, Matthew Wright, Lindsay Victoria, Benjamin Zebley, Joshua Levitz, Corey J. Keller, Aaron D. Boes, Conor Liston

Journal:
Cell
Publication Date:
Research Area:
Neural Circuits
Tissue Type:
Brain
Products Cited:
SHIELD, SmartSPIM
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