Tissue Clearing Reagents

12 SmartBatch+-processed mouse brains before delipidation
Before delipidation
12 SmartBatch+-processed mouse brains after delipidation and index-matching
After index-matching
12 SmartBatch+-processed mouse brains immunolabeled with NeuN
After immunolabeling & imaging

Clear+ tissue clearing system

Our new Clear+ tissue clearing technique is the only method that delipidates and optically clears samples with no change in sample morphology and with minimal impact on structural integrity. Samples processed with our tissue clearing reagents retain mechanical strength, molecular stability, and native 3D structure.

An advance on our founder’s CLARITY method, this targeted delipidation approach is also dramatically faster: fully delipidate whole mouse brains in just one day with SmartBatch+ active clearing, or just one week with passive clearing. Clear+ can also delipidate larger samples like whole rat brains in ~4 days.

After clearing, leverage our active immunolabeling methods to rapidly and uniformly label whole organs from surface to core with significantly less antibody than passive techniques like iDISCO and CUBIC.

Tissue Clearing - Highlighted pipeline icon

Passive Clearing Kit

Start with the most versatile passive clearing method available: preserve, clear, and index-match your samples with our simple and reliable ready-made solutions, now featuring the Clear+ system.

Our reagents preserve fluorescent proteins, reduce autofluorescence, and optimally prepare samples for immunostaining, 3D imaging, and analysis. For significantly faster clearing speed and sample throughput, upgrade to electrophoretic clearing using SmartBatch+.

Tissue Preservation + Tissue Clearing - Highlighted pipeline icons

Kit Sizes

250 mL: Processes 8+ mouse brains or comparably sized samples. Includes: 250 mL SHIELD, 250 mL Clear+ Delipidation Buffer, and 250 mL EasyIndex.

500 mL: Processes 16+ mouse brains or comparably sized samples. Includes: 500 mL SHIELD, 500 mL Clear+ Delipidation Buffer, and 500 mL EasyIndex.

Recent publications citing LifeCanvas tissue clearing reagents

Adeno-associated virus delivered CXCL9 sensitizes glioblastoma to anti-PD-1 immune checkpoint blockade

Christina A. von Roemeling, Jeet A. Patel, Savannah L. Carpenter, Oleg Yegorov, Changlin Yang, Alisha Bhatia, Bently P. Doonan, Rylynn Russell, Vrunda S. Trivedi, Kelena Klippel, Daniel H. Ryu, Adam Grippin, Hunter S. Futch, Yong Ran, Lan B. Hoang-Minh, Frances L. Weidert, Todd E. Golde & Duane A. Mitchell

Journal:
Nature Communications
Publication Date:
Products Cited:
SmartBatch+, Passive Clearing Kit, SmartSPIM, CRO Services
Serine racemase deletion alters adolescent social behavior and whole-brain cFos activation

Stephanie E. Brown, Ziyi (Zephyr) Wang, Emily L. Newman, Elif Engin, Sabina Berretta, Darrick T. Balu, Oluwarotimi O. Folorunso.

Journal:
Frontiers in Psychiatry
Publication Date:
Research Area:
cFos, Mapping
Tissue Species:
Mouse
Tissue Type:
Heart
Products Cited:
SHIELD, SmartBatch+, SmartSPIM, CRO Services
A cocaine-activated ensemble exerts increased control over behavior while decreasing in size

Kimberly C. Thibeault, Michael Z. Leonard, Veronika Kondev, Soren D. Emerson, Rishik Bethi, Alberto J. Lopez, Jonathon P. Sens, Brett P. Nabit, Hannah B. Elam, Danny G. Winder, Sachin Patel, Drew D. Kiraly, Brad A. Grueter, Erin S. Calipari.

Journal:
Biological Psychiatry
Publication Date:
Research Area:
cFos, Mapping
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, SmartBatch+, SmartSPIM, CRO Services

Further reading