Here, we demonstrate how the Megatome Premium can easily facilitate this process through whole-mount and non-destructive tissue sectioning.
Our new Clear+ tissue clearing technique is the only method that delipidates and optically clears samples with no change in sample morphology and with minimal impact on structural integrity. Samples processed with our tissue clearing reagents retain mechanical strength, molecular stability, and native 3D structure.
An advance on our founder’s CLARITY method, this targeted delipidation approach is also dramatically faster: fully delipidate whole mouse brains in just one day with SmartBatch+ active clearing, or just one week with passive clearing. Clear+ can also delipidate larger samples like whole rat brains in ~4 days.
After clearing, leverage our active immunolabeling methods to rapidly and uniformly label whole organs from surface to core with significantly less antibody than passive techniques like iDISCO and CUBIC.
Start with the most versatile passive clearing method available: preserve, clear, and index-match your samples with our simple and reliable ready-made solutions, now featuring the Clear+ system.
Our reagents preserve fluorescent proteins, reduce autofluorescence, and optimally prepare samples for immunostaining, 3D imaging, and analysis. For significantly faster clearing speed and sample throughput, upgrade to electrophoretic clearing using SmartBatch+.
250 mL: Processes 8+ mouse brains or comparably sized samples. Includes: 250 mL SHIELD, 250 mL Clear+ Delipidation Buffer, and 250 mL EasyIndex.
500 mL: Processes 16+ mouse brains or comparably sized samples. Includes: 500 mL SHIELD, 500 mL Clear+ Delipidation Buffer, and 500 mL EasyIndex.
Here, we demonstrate how the Megatome Premium can easily facilitate this process through whole-mount and non-destructive tissue sectioning.
Immunohistochemistry (IHC) and 3D histology are different approaches to detecting specific proteins in biological tissue.
Tissue clearing techniques have long since sought to maximize optical transparency while preserving sample architecture and minimizing processing time. While...
LifeCanvas tissue clearing is fast, reliable, and cost-effective, allowing uniform and simultaneous clearing for multiple samples.