Tissue Clearing Reagents

12 SmartBatch+-processed mouse brains before delipidation
Before delipidation
12 SmartBatch+-processed mouse brains after delipidation and index-matching
After index-matching
12 SmartBatch+-processed mouse brains immunolabeled with NeuN
After immunolabeling & imaging

Clear+ tissue clearing system

Our new Clear+ tissue clearing technique is the only method that delipidates and optically clears samples with no change in sample morphology and with minimal impact on structural integrity. Samples processed with our tissue clearing reagents retain mechanical strength, molecular stability, and native 3D structure.

An advance on our founder’s CLARITY method, this targeted delipidation approach is also dramatically faster: fully delipidate whole mouse brains in just one day with SmartBatch+ active clearing, or just one week with passive clearing. Clear+ can also delipidate larger samples like whole rat brains in ~4 days.

After clearing, leverage our active immunolabeling methods to rapidly and uniformly label whole organs from surface to core with significantly less antibody than passive techniques like iDISCO and CUBIC.

Tissue Clearing - Highlighted pipeline icon

Passive Clearing Kit

Start with the most versatile passive clearing method available: preserve, clear, and index-match your samples with our simple and reliable ready-made solutions, now featuring the Clear+ system.

Our reagents preserve fluorescent proteins, reduce autofluorescence, and optimally prepare samples for immunostaining, 3D imaging, and analysis. For significantly faster clearing speed and sample throughput, upgrade to electrophoretic clearing using SmartBatch+.

Tissue Preservation + Tissue Clearing - Highlighted pipeline icons

Kit Sizes

250 mL: Processes 8+ mouse brains or comparably sized samples. Includes: 250 mL SHIELD, 250 mL Clear+ Delipidation Buffer, and 250 mL EasyIndex.

500 mL: Processes 16+ mouse brains or comparably sized samples. Includes: 500 mL SHIELD, 500 mL Clear+ Delipidation Buffer, and 500 mL EasyIndex.

Recent publications citing LifeCanvas tissue clearing reagents

Loss of insulin signaling in astrocytes exacerbates Alzheimer-like phenotypes in a 5xFAD mouse model

Wenqiang Chen, Qian Huang, Ekaterina Katie Lazdon, Antonio Gomes, Marisa Wong, Emily Stephens, Tabitha Grace Royal, Dan Frenkel, Weikang Cai, C. Ronald Kahn

Journal:
PNAS
Publication Date:
Research Area:
Neurodegeneration, Metabolism
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, Passive Clearing Kit, SmartSPIM, SmartAnalytics, CRO Services
Leveraging the Power of 3D Brain-Wide Imaging and Mapping Tools for Brain Injury Research in Murine Models

Mehwish Anwer, Jeffrey LeDue, Zefang Wang, Sarah Wang, Wai Hang Cheng, Mariia Burdyniuk, Honor Cheung, Jianjia Fan, Carlos Barron, Peter A. Cripton, Mark S. Cembrowski, Fabio Rossi, Timothy H. Murphy, Cheryl L. Wellington

Journal:
bioRxiv
Publication Date:
Research Area:
Brain Injury, Methods
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, SmartAnalytics, CRO Services
BNST PKCδ neurons are activated by specific aversive conditions to promote anxiety-like behavior

Kellie M. Williford, Anne Taylor, James R. Melchior, Hye Jean Yoon, Eryn Sale, Milen D. Negasi, Danielle N. Adank, Jordan A. Brown, Michelle N. Bedenbaugh, Joseph R. Luchsinger, Samuel W. Centanni, Sachin Patel, Erin S. Calipari, Richard B. Simerly, Danny G. Winder

Journal:
Neuropsychopharmacology
Publication Date:
Research Area:
Neural Circuits
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, Passive Clearing Kit, SmartSPIM, SmartAnalytics

Further reading