Tissue Clearing Reagents

12 SmartBatch+-processed mouse brains before delipidation
Before delipidation
12 SmartBatch+-processed mouse brains after delipidation and index-matching
After index-matching
12 SmartBatch+-processed mouse brains immunolabeled with NeuN
After immunolabeling & imaging

Clear+ tissue clearing system

Our new Clear+ tissue clearing technique is the only method that delipidates and optically clears samples with no change in sample morphology and with minimal impact on structural integrity. Samples processed with our tissue clearing reagents retain mechanical strength, molecular stability, and native 3D structure.

An advance on our founder’s CLARITY method, this targeted delipidation approach is also dramatically faster: fully delipidate whole mouse brains in just one day with SmartBatch+ active clearing, or just one week with passive clearing. Clear+ can also delipidate larger samples like whole rat brains in ~4 days.

After clearing, leverage our active immunolabeling methods to rapidly and uniformly label whole organs from surface to core with significantly less antibody than passive techniques like iDISCO and CUBIC.

Tissue Clearing - Highlighted pipeline icon

Passive Clearing Kit

Start with the most versatile passive clearing method available: preserve, clear, and index-match your samples with our simple and reliable ready-made solutions, now featuring the Clear+ system.

Our reagents preserve fluorescent proteins, reduce autofluorescence, and optimally prepare samples for immunostaining, 3D imaging, and analysis. For significantly faster clearing speed and sample throughput, upgrade to electrophoretic clearing using SmartBatch+.

Tissue Preservation + Tissue Clearing - Highlighted pipeline icons

Kit Sizes

250 mL: Processes 8+ mouse brains or comparably sized samples. Includes: 250 mL SHIELD, 250 mL Clear+ Delipidation Buffer, and 250 mL EasyIndex.

500 mL: Processes 16+ mouse brains or comparably sized samples. Includes: 500 mL SHIELD, 500 mL Clear+ Delipidation Buffer, and 500 mL EasyIndex.

Recent publications citing LifeCanvas tissue clearing reagents

A Light sheet fluorescence microscopy and machine learning-based approach to investigate drug and biomarker distribution in whole organs and tumors

Niyanta Kumar, Petr Hrobar, Martin Vagenknecht, Jindrich Soukup, Nadia Patterson, Peter Bloomingdale, Tomoko Freshwater, Sophia Bardehle, Roman Peter, Ruban Mangadu, Cinthia V Pastuskovas, Chiswili Yves Chabu, Mark T. Cancilla

Journal:
bioRxiv
Publication Date:
Research Area:
Neurodegeneration, Cancer, Vasculature, Methods
Tissue Species:
Mouse
Tissue Type:
Brain, Tumor
Products Cited:
SHIELD, EasyIndex, SmartClear, SmartLabel, SmartSPIM, SmartAnalytics, CRO Services
Developmental Mouse Brain Common Coordinate Framework

Fae A. Kronman, Josephine K. Liwang, Rebecca Betty, Daniel J. Vanselow, Yuan-ting Wu, Nicholas J. Tustison, Ashwin Bhandiwad, Steffy B. Manjila, Jennifer A. Minteer, Donghui Shin, Choong Heon Lee, Rohan Patil, Jeffrey T. Duda, Luis Puelles, James C. Gee, Jiangyang Zhang, Lydia Ng, Yongsoo Kim

Journal:
bioRxiv
Publication Date:
Research Area:
Methods, Other
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartClear, SmartLabel, Passive Clearing Kit, SmartSPIM
Drugs of abuse hijack a mesolimbic pathway that processes homeostatic need

Bowen Tan, Caleb J. Browne, Tobias Nöbauer, Alipasha Vaziri, Jeffrey M. Friedman, Eric J. Nestler

Journal:
bioRxiv
Publication Date:
Research Area:
Neural Circuits
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, CRO Services

Further reading