SmartSPIM

Light sheet microscope built for intact tissues

With uniform axial resolution across the whole sample, rapid volumetric imaging, and a custom imaging chamber, our SmartSPIM light sheet microscope provides the accuracy, speed, and flexibility you need to generate the highest quality data. Unlike many light sheet microscopes, SmartSPIM makes no compromises on resolution or acquisition speed.

Chosen by leading research institutions

Technical specifications

Uniform axial resolution across FOV

The SmartSPIM light sheet microscope leverages patented axial sweeping technology (Dean et al. 2015): illumination optics scan the beam while synchronized to the camera’s rolling shutter detection, providing homogeneous axial point spread functions (PSFz) across the entire field of view (FOV)

Left: The sample imaged without axial sweeping displays high axial resolution only at the center of the FOV, while the sample imaged with sweeping shows uniform axial resolution across the FOV.

Microglia (IBA-1, 647 nm) in cortex of a 7-month-old wild-type C57BL/6 male mouse from Taconic Biosciences, model #B6, 3.6X magnification.

Customizable imaging chamber

Custom-designed imaging chamber and sample holders can accommodate a variety of tissue sizes, from organoids to rat brains, and even entire juvenile mice! With no points of contact between tissue and mounting hardware, the user can mount many sample types and sizes in different orientations.

Rapid, intuitive volumetric acquisition

Tunable imaging parameters allow for high speed volumetric imaging while maintaining quality resolution. 20 fps acquisition allows imaging of a mouse brain hemisphere or comparably sized intact sample in <30 minutes (at 1.8 µm x 1.8 µm x 4 µm voxel size).

Streamlined for efficient data collection, SmartSPIM’s acquisition software has real-time tile correction and is simple to operate, making imaging large samples a breeze.

Syto16 (magenta) and vimentin (cyan) in a day 50 dorsal forebrain organoid from STEMCELL Technologies generated using the STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit (cat. #08620), 15X magnification.

Further reading

LifeCanvas Portraits: Dr. Yongsoo Kim

Dr. Kim’s lab recently published “Whole-Brain Wiring Diagram of Oxytocin System in Adult Mice” in The Journal of Neuroscience. LifeCanvas...

LifeCanvas Portraits: Dr. Li Ye

Updated April 13, 2022: Dr. Ye’s lab recently published “HYBRiD: hydrogel-reinforced DISCO for clearing mammalian bodies.” Learn more about the...

LifeCanvas Portraits: Dr. Jose Maldonado

Jose Maldonado, Managing Director of Vanderbilt’s NeuroVisualization Lab, discusses his neuroscience research interests and how LifeCanvas’ imaging and analysis platforms...

Header: β-amyloid disease plaques in magenta, astrocytes (GFAP) in cyan, 15X magnification. Sample courtesy of Drs. David Elliott and Jonathan Epp, University of Calgary.