SmartSPIM

Light sheet microscope built for intact tissues

With uniform axial resolution across the whole sample, rapid volumetric imaging, and a custom imaging chamber, our SmartSPIM light sheet microscope provides the accuracy, speed, and flexibility you need to generate the highest quality data. Unlike many light sheet microscopes, SmartSPIM makes no compromises on resolution or acquisition speed.

Light Sheet Microscopy - Highlighted pipeline icon
SmartSPIM light sheet microscope

Chosen by leading research institutions

Uniform axial resolution across FOV

The SmartSPIM light sheet microscope leverages patented axial sweeping technology (Dean et al. 2015): illumination optics scan the beam while synchronized to the camera’s rolling shutter detection, providing homogeneous axial point spread functions (PSFz) across the entire field of view (FOV)

Left: The sample imaged without axial sweeping displays high axial resolution only at the center of the FOV, while the sample imaged with sweeping shows uniform axial resolution across the FOV.

Microglia (IBA-1, 647 nm) in cortex of a 7-month-old wild-type C57BL/6 male mouse from Taconic Biosciences, model #B6, 3.6X objective.

Customizable imaging chamber

Custom-designed imaging chamber and sample holders can accommodate a variety of tissue sizes, from organoids to rat brains, and even entire juvenile mice! With no points of contact between tissue and mounting hardware, the user can mount many sample types and sizes in different orientations.

Rapid, intuitive volumetric acquisition

Tunable imaging parameters allow for high speed volumetric imaging while maintaining quality resolution. 20 fps acquisition allows imaging of a mouse brain hemisphere or comparably sized intact sample in <30 minutes (at 1.8 µm x 1.8 µm x 4 µm voxel size).

Streamlined for efficient data collection, SmartSPIM’s acquisition software has real-time tile correction and is simple to operate, making imaging large samples a breeze.

Syto16 and vimentin in intact day 50 dorsal forebrain organoid
Syto16 (magenta) and vimentin (cyan) in a day 50 dorsal forebrain organoid from STEMCELL Technologies generated using the STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit (cat. #08620), 15X objective.

Technical specifications

SmartSPIM light sheet microscope specifications

More than a microscope – a complete solution

Tissue Processing

Prepare samples with tools for preservation, clearing, labeling, & sectioning.

Light Sheet Imaging

Acquire spatially informative images with innovative microscopy techniques.

Image Analysis

Generate publication-quality data with robust analysis tools.

Recent publications citing the SmartSPIM light sheet microscope

BNST PKCδ neurons are activated by specific aversive conditions to promote anxiety-like behavior

Kellie M. Williford, Anne Taylor, James R. Melchior, Hye Jean Yoon, Eryn Sale, Milen D. Negasi, Danielle N. Adank, Jordan A. Brown, Michelle N. Bedenbaugh, Joseph R. Luchsinger, Samuel W. Centanni, Sachin Patel, Erin S. Calipari, Richard B. Simerly, Danny G. Winder

Journal:
Neuropsychopharmacology
Publication Date:
Research Area:
Neural Circuits
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, Passive Clearing Kit, SmartSPIM, SmartAnalytics
Characterizing the differential distribution and targets of Sumo1 and Sumo2 in the mouse brain

Terry R. Suk, Trina T. Nguyen, Zoe A. Fisk, Miso Mitkovski, Haley M. Geertsma, Jean-Louis A. Parmasad, Meghan M. Heer, Steve M. Callaghan, Fritz Benseler, Nils Brose, Marilyn Tirard, Maxime W.C. Rousseaux

Journal:
iScience
Publication Date:
Research Area:
Other
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, CRO Services
Forty-hertz light stimulation does not entrain native gamma oscillations in Alzheimer’s disease model mice

Marisol Soula, Alejandro Martín-Ávila, Yiyao Zhang, Annika Dhingra, Noam Nitzan, Martin J. Sadowski, Wen-Biao Gan, György Buzsáki

Journal:
Nature
Publication Date:
Research Area:
Neurodegeneration
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartLabel, Passive Clearing Kit, SmartSPIM, SmartAnalytics, CRO Services

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Further reading

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The Allen Brain Atlas is an incredibly powerful tool for neuroscience research, enabling researchers to discuss their findings in a...

Header: β-amyloid disease plaques in magenta, astrocytes (GFAP) in cyan, 15X objective. Sample courtesy of Drs. David Elliott and Jonathan Epp, University of Calgary.