SmartSPIM

Light sheet microscope built for intact tissues

With uniform axial resolution across the whole sample, rapid volumetric imaging, and a custom imaging chamber, our SmartSPIM light sheet microscope provides the accuracy, speed, and flexibility you need to generate the highest quality data. Unlike many light sheet microscopes, SmartSPIM makes no compromises on resolution or acquisition speed.

Header: β-amyloid disease plaques in magenta, astrocytes (GFAP) in cyan, 15X magnification. Sample courtesy of Drs. David Elliott and Jonathan Epp, University of Calgary.

Technical specifications

Uniform axial resolution across FOV

The SmartSPIM light sheet microscope leverages patented axial sweeping technology (Dean et al. 2015): illumination optics scan the beam while synchronized to the camera’s rolling shutter detection, providing homogeneous axial point spread functions (PSFz) across the entire field of view (FOV)

Left: The sample imaged without axial sweeping displays high axial resolution only at the center of the FOV, while the sample imaged with sweeping shows uniform axial resolution across the FOV.

Microglia (IBA-1, 647 nm) in cortex of a 7-month-old wild-type C57BL/6 male mouse from Taconic Biosciences, model #B6, 3.6X magnification.

Customizable imaging chamber

Custom-designed imaging chamber and sample holders can accommodate a variety of tissue sizes, from organoids to rat brains, and even entire juvenile mice! With no points of contact between tissue and mounting hardware, the user can mount many sample types and sizes in different orientations.

Rapid, intuitive volumetric acquisition

Tunable imaging parameters allow for high speed volumetric imaging while maintaining quality resolution. 20 fps acquisition allows imaging of a mouse brain hemisphere or comparably sized intact sample in <30 minutes (at 1.8 µm x 1.8 µm x 4 µm voxel size).

Streamlined for efficient data collection, SmartSPIM’s acquisition software has real-time tile correction and is simple to operate, making imaging large samples a breeze.

Ready for image analysis? Easily craft publication-ready analysis outputs with our SmartAnalytics workstation, tailor-made for SmartSPIM.
Syto16 (magenta) and vimentin (cyan) in a day 50 dorsal forebrain organoid from STEMCELL Technologies generated using the STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit (cat. #08620), 15X magnification.
"Working with colleagues at LifeCanvas Technologies has opened an entirely new avenue of research for us. Our interest has focused on increasing the spatial resolution in magnetic resonance histology — microscopic imaging of the rodent brain, to allow studies of the mouse brain connectome. Using the clearing/light sheet with the Thy 1 mouse has yielded in the same specimen, diffusion tensor MRI images and the opportunity to validate specific motor pathways. Merging the two yields a sum that is far more than the individual parts. We see the light sheet data from our colleagues at LifeCanvas Technologies as a critical new complement to our MR methodologies. Our colleagues there have been more than helpful and we look forward to extended collaboration in the future."
G Allan Johnson, Ph.D.
Charles E Putman Professor of Radiology,
Duke University School of Medicine

Recent publications citing SmartSPIM