Light sheet fluorescence and laser scanning confocal microscopy are both used to acquire 3D image datasets with optical sectioning, however...
Menu
With uniform axial resolution across the whole sample, rapid volumetric imaging, and a custom imaging chamber, our SmartSPIM light sheet microscope provides the accuracy, speed, and flexibility you need to generate the highest quality data. Unlike many light sheet microscopes, SmartSPIM makes no compromises on resolution or acquisition speed.
The SmartSPIM light sheet microscope leverages patented axial sweeping technology (Dean et al. 2015): illumination optics scan the beam while synchronized to the camera’s rolling shutter detection, providing homogeneous axial point spread functions (PSFz) across the entire field of view (FOV).
Left: The sample imaged without axial sweeping displays high axial resolution only at the center of the FOV, while the sample imaged with sweeping shows uniform axial resolution across the FOV.
Microglia (IBA-1, 647 nm) in cortex of a 7-month-old wild-type C57BL/6 male mouse from Taconic Biosciences, model #B6, 3.6X objective.
Custom-designed imaging chamber and sample holders can accommodate a variety of tissue sizes, from organoids to rat brains, and even entire juvenile mice! With no points of contact between tissue and mounting hardware, the user can mount many sample types and sizes in different orientations.
Tunable imaging parameters allow for high speed volumetric imaging while maintaining quality resolution. 20 fps acquisition allows imaging of a mouse brain hemisphere or comparably sized intact sample in <30 minutes (at 1.8 µm x 1.8 µm x 4 µm voxel size).
Streamlined for efficient data collection, SmartSPIM’s acquisition software has real-time tile correction and is simple to operate, making imaging large samples a breeze.
Prepare samples with tools for preservation, clearing, labeling, & sectioning.
Acquire spatially informative images with innovative microscopy techniques.
Generate publication-quality data with robust analysis tools.
Light sheet fluorescence and laser scanning confocal microscopy are both used to acquire 3D image datasets with optical sectioning, however...
The Allen Brain Atlas is an incredibly powerful tool for neuroscience research, enabling researchers to discuss their findings in a...
Dr. Michelle Bedenbaugh uses SmartSPIM microscopy and SmartAnalytics to visualize and quantify MC3R circuitry in whole mouse brains.
Dr. Kim's lab at Penn State uses the SmartSPIM light sheet microscope to map and create 3D atlases of the...
Header: β-amyloid disease plaques in magenta, astrocytes (GFAP) in cyan, 15X objective. Sample courtesy of Drs. David Elliott and Jonathan Epp, University of Calgary.