EasyIndex

Optical Clearing Solution

Our 500-mL bottle can be used for up to 12 whole mouse brains or 200+ 1 mm-thick tissue sections! EasyIndex is the most economical option guaranteeing best refractive index matching results (RI = 1.52 standard; RI = 1.46 available on request). Intact tissues are rendered optically transparent and are optimally prepared for volumetric imaging with SmartSPIM or other microscopes.

EasyIndex is ideal for use with:

Cleared tissues using aqueous-based methods and others
Immunolabeled tissues

Download the protocol below to learn more about the entire LifeCanvas tissue processing system, including refractive index matching with EasyIndex.

Cerebral organoid delipidated and refractive index matched on light grid — Cerebral organoid

Mouse brain delipidated and refractive index matched on light grid — Mouse brain

Human skin sample delipidated and refractive index matched on light grid — Human skin

Mouse colon delipidated and refractive index matched on light grid — Mouse colon

Mouse embryo delipidated and refractive index matched on light grid — Mouse embryo

Mouse spinal cord delipidated and refractive index matched on light grid — Mouse spinal cord

Mouse heart delipidated and refractive index matched on light grid — Mouse heart

Mouse intestine delipidated and refractive index matched on light grid — Mouse intestine

Mouse kidney delipidated and refractive index matched on light grid — Mouse kidney

Rat lung tissue delipidated and refractive index matched on light grid — Rat lung

Mouse spleen delipidated and refractive index matched on light grid — Mouse spleen

Mouse liver delipidated and refractive index matched on light grid — Mouse liver

Rat brain hemisphere delipidated and refractive index matched on light grid — Rat brain hemisphere

Examples of cleared and RI-matched tissue types from our tissue processing laboratory. Cleared (delipidated) tissues will appear translucent, while cleared and refractive index-matched tissues will appear transparent.

Removal of lipid molecules and refractive index matching allows light to pass unhindered through the sample during microscopy, producing sharp, informative images even in regions deep within the tissue.