While developing our next generation of electrophoretic tissue-processing devices, SmartBatch+, we have also been hard at work developing improvements to...
Delipidation and refractive index (RI) -matching allow light to pass unhindered through the sample during microscopy. In effect, tissue clearing refers to the combined process of delipidation and optical clearing via index matching.
Delipidated tissues will appear translucent, while cleared and RI-matched tissues will appear transparent. Our preformulated EasyIndex solution prepares samples for sharp, informative volumetric imaging with light sheet microscopes like SmartSPIM.
The 500-mL EasyIndex bottle can be used for up to 12 whole mouse brains or similarly sized intact samples, or >200 1 mm-thick sections. We provide RI = 1.52 standard solutions with RI = 1.46 solution available on request.
EasyIndex is ideal for index-matching multiple tissue types, immunolabeled tissues, and specimens cleared with aqueous methods like SHIELD or CLARITY.
While developing our next generation of electrophoretic tissue-processing devices, SmartBatch+, we have also been hard at work developing improvements to...
We discuss the second part of the tissue processing journey: active tissue clearing using SmartClear. SmartClear active clearing is rapid,...
Over 190 million people have now been infected with the novel coronavirus SARS-CoV-2 in the ongoing coronavirus disease (COVID-19) pandemic...
As explained in several of our blogs, delipidation is a critical step in the tissue clearing process. Some form of...