EasyIndex

Optimized refractive index matching

Delipidation and refractive index (RI) -matching allow light to pass unhindered through the sample during microscopy. In effect, tissue clearing refers to the combined process of delipidation and optical clearing via index matching.

Delipidated tissues will appear translucent, while cleared and RI-matched tissues will appear transparent. Our preformulated EasyIndex solution prepares samples for sharp, informative volumetric imaging with light sheet microscopes like SmartSPIM.

EasyIndex2_100dpi

Optical clearing of intact tissues & sections

The 500-mL EasyIndex bottle can be used for up to 12 whole mouse brains or similarly sized intact samples, or >200 1 mm-thick sections. We provide RI = 1.52 standard solutions with RI = 1.46 solution available on request.

EasyIndex is ideal for index-matching multiple tissue types, immunolabeled tissues, and specimens cleared with aqueous methods like SHIELD or CLARITY.

Recent publications citing EasyIndex

Shared and Distinct Brain Regions Targeted for Immediate Early Gene Expression by Ketamine and Psilocybin

Pasha A. Davoudian, Ling-Xiao Shao, Alex C. Kwan

Journal:
ACS Chemical Neuroscience
Publication Date:
Research Area:
Neural Circuits
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, CRO Services
Whole-Brain Clearing and Immunolabelling in the African Killifish Nothobranchius furzeri

Augusto Ortega Granillo, Fengyan Deng, Hannah Wilson, Yongfu Wang, Alejandro Sánchez Alvarado

Journal:
Emerging Model Organisms
Publication Date:
Research Area:
Methods
Tissue Species:
Killifish
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartClear, SmartLabel
Arachnoid granulations are lymphatic conduits that communicate with bone marrow and dura-arachnoid stroma

Trishna Shah, Sue E. Leurgans, Rashi I. Mehta, Jingyun Yang, Chad A. Galloway, Karen L. de Mesy Bentley, Julie A. Schneider, Rupal I. Mehta

Journal:
Journal of Experimental Medicine
Publication Date:
Research Area:
Other
Tissue Species:
Human
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartClear, SmartLabel, SmartSPIM

Further reading