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Delipidation and refractive index (RI) matching allow light to pass unhindered through the sample during microscopy. In effect, tissue clearing refers to the combined process of delipidation and optical clearing via index matching.
Delipidated tissues will appear translucent, while cleared and RI-matched tissues will appear transparent. Our preformulated EasyIndex solution prepares samples for sharp, informative volumetric imaging with light sheet microscopes like SmartSPIM.
The 500-mL EasyIndex bottle can be used for up to 12 whole mouse brains or similarly sized intact tissue samples, or >200 1 mm-thick tissue sections. We provide RI = 1.52 standard solutions with RI = 1.46 solution available on request.
EasyIndex is ideal for optical clearing of multiple tissue types, immunolabeled tissues, and tissues cleared with aqueous methods like SHIELD or CLARITY.