EasyIndex

Whole mouse brain before delipidation, after delipidation, and after optical clearing including refractive index matching

Refractive index matching, simplified

Delipidation and refractive index (RI) -matching allow light to pass unhindered through the sample during microscopy. In effect, tissue clearing refers to the combined process of delipidation and optical clearing via index matching.

Delipidated tissues will appear translucent, while cleared and RI-matched tissues will appear transparent. Our preformulated EasyIndex solution prepares samples for sharp, informative volumetric imaging with light sheet microscopes like SmartSPIM and MegaSPIM.

Tissue Clearing - Highlighted pipeline icon
EasyIndex bottle

Optically clear intact tissues and sections

The 500-mL EasyIndex bottle can be used for up to 12 whole mouse brains or similarly sized intact samples, or >200 1 mm-thick sections. We provide RI = 1.52 standard solutions with RI = 1.46 solution available on request.

EasyIndex is ideal for index-matching multiple tissue types, immunolabeled tissues, and specimens cleared with aqueous methods like SHIELD or CLARITY.

Recent publications citing EasyIndex

BNST PKCδ neurons are activated by specific aversive conditions to promote anxiety-like behavior

Kellie M. Williford, Anne Taylor, James R. Melchior, Hye Jean Yoon, Eryn Sale, Milen D. Negasi, Danielle N. Adank, Jordan A. Brown, Michelle N. Bedenbaugh, Joseph R. Luchsinger, Samuel W. Centanni, Sachin Patel, Erin S. Calipari, Richard B. Simerly, Danny G. Winder

Journal:
Neuropsychopharmacology
Publication Date:
Research Area:
Neural Circuits
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, Passive Clearing Kit, SmartSPIM, SmartAnalytics
Intra-tracheally injected human induced pluripotent stem cell-derived pneumocytes and endothelial cells engraft in the distal lung and ameliorate emphysema in a rat model

Wafa Altalhi, Tong Wu, Gregory R. Wojtkiewicz, Sydney Jeffs, Kenji Miki, Harald C. Ott

Journal:
Journal of Thoracic and Cardiovascular Surgery
Publication Date:
Research Area:
Stem Cells, Other
Tissue Species:
Rat
Tissue Type:
Lung
Products Cited:
EasyIndex
Characterizing the differential distribution and targets of Sumo1 and Sumo2 in the mouse brain

Terry R. Suk, Trina T. Nguyen, Zoe A. Fisk, Miso Mitkovski, Haley M. Geertsma, Jean-Louis A. Parmasad, Meghan M. Heer, Steve M. Callaghan, Fritz Benseler, Nils Brose, Marilyn Tirard, Maxime W.C. Rousseaux

Journal:
iScience
Publication Date:
Research Area:
Other
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, CRO Services

Further reading