SmartBatch+

Electrophoretic tissue clearing & immunolabeling

Our easy-to-use system combines active tissue clearing and immunolabeling into one high-throughput device, allowing you to switch between clearing and labeling modes simply by changing buffers. SmartBatch+ is compatible with different tissue types and large intact samples.

An advance on our founder’s CLARITY tissue clearing method, Clear+ provides maximum optical transparency with no tissue expansion or contraction. In immunolabeling mode, eFLASH and patented SE technologies uniformly label whole organs. Unlike other methods like iDISCO and CUBIC, SmartBatch+ actively preserves fluorescent protein signal.

Consistent histological data

SmartBatch+ actively clears and labels up to 12 whole mouse brains or comparably sized samples in as little as one day, and 2 whole rat brains in just 3-4 days! This provides unparalleled consistency for multiple treatment groups. Its small footprint also allows ample space for multiple devices.

High quality sample integrity for 3D imaging

SmartBatch+ combined with SHIELD tissue preservation protects fluorescent protein signal, antigenicity, and molecular and physical architecture through all tissue processing steps. Clear+ is currently the only tissue clearing technique that preserves sample morphology. This ensures your samples will produce information-rich images.

Fast, cost-effective clearing & immunolabeling

Use just 4-20 μg of antibody per target to label a sample the size of a whole mouse brain — up to an order of magnitude less than passive staining. Save hundreds of dollars per sample per antibody, in primary antibody alone! SmartBatch+’s turnkey design is also labor-efficient, requiring <12 mins hands-on time per sample to clear and label.

Recent publications citing SmartBatch+

Shared and Distinct Brain Regions Targeted for Immediate Early Gene Expression by Ketamine and Psilocybin

Pasha A. Davoudian, Ling-Xiao Shao, Alex C. Kwan

Journal:
ACS Chemical Neuroscience
Publication Date:
Research Area:
Neural Circuits
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, CRO Services
Cardiovascular baroreflex circuit moonlights in sleep control

Yuanyuan Yao, Zeke Barger, Mohammad Saffari Doost, Chak Foon Tso, Dana Darmohray, Daniel Silverman, Danqian Liu, Chenyan Ma, Ali Cetin, Shenqin Yao, Hongkui Zeng, Yang Dan

Journal:
Neuron
Publication Date:
Research Area:
Neural Circuits
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, CRO Services
Characterizing the differential distribution and targets of Sumo paralogs in the mouse brain

Terry R. Suk, Trina T. Nguyen, Zoe A. Fisk, Miso Mitkovski, Haley M. Geertsma, Jean-Louis A. Parmasad, Meghan M. Heer, Steve M. Callaghan, Nils Brose, Marilyn Tirard, Maxime W.C. Rousseaux

Journal:
bioRxiv
Publication Date:
Research Area:
Neurodegeneration
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, CRO Services

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