Switches between clearing and labeling modes simply by changing buffers. Clears or labels 12 mouse brains or comparably sized samples in 1 day. Compatible with different tissue types and large intact samples.
Capable of actively clearing or labeling up to 12 whole mouse brains, 2 whole rat brains, or comparably sized samples per batch. Provides consistency for multiple treatment groups.
Uses 4-20 μg of antibody per target to label a sample the size of a whole mouse brain. Clearing buffers last up to 1 week, enabling continuous batch clearing without changing reagents.
Anti-NeuN comparison for 1 whole mouse brain:
Stochastic electrotransport combined with SHIELD preserves molecular and physical architecture through all tissue processing steps, and there is no tissue expansion after clearing.