SmartBatch+

Electrophoretic tissue clearing & immunolabeling

Our easy-to-use system combines active tissue clearing and immunolabeling into one high-throughput device, allowing you to switch between clearing and labeling modes simply by changing buffers. SmartBatch+ is compatible with different tissue types and large intact samples.

An advance on our founder’s CLARITY tissue clearing method, Clear+ provides maximum optical transparency with no tissue expansion or contraction. In immunolabeling mode, eFLASH and patented SE technologies uniformly label whole organs. Unlike other methods like iDISCO and CUBIC, SmartBatch+ actively preserves fluorescent protein signal.

Tissue Clearing + Tissue Labeling - Highlighted pipeline icons

Consistent histological data

SmartBatch+ actively clears and labels up to 12 whole mouse brains or comparably sized samples in as little as one day, and 2 whole rat brains in just 3-4 days! This provides unparalleled consistency for multiple treatment groups. Its small footprint also allows ample space for multiple devices.

High quality sample integrity for 3D imaging

SmartBatch+ combined with SHIELD tissue preservation protects fluorescent protein signal, antigenicity, and molecular and physical architecture through all tissue processing steps. Clear+ is currently the only tissue clearing technique that preserves sample morphology. This ensures your samples will produce information-rich images.

Fast, cost-effective tissue clearing & labeling

Use just 4-20 μg of antibody per target to label a sample the size of a whole mouse brain — up to an order of magnitude less than passive staining. Save hundreds of dollars per sample per antibody, in primary antibody alone! SmartBatch+’s turnkey design is also labor-efficient, requiring <12 mins hands-on time per sample to clear and label.

SmartBatch+ tissue clearing and labeling workflow

Recent publications citing SmartBatch+

Drugs of abuse hijack a mesolimbic pathway that processes homeostatic need

Bowen Tan, Caleb J. Browne, Tobias Nöbauer, Alipasha Vaziri, Jeffrey M. Friedman, Eric J. Nestler

Journal:
bioRxiv
Publication Date:
Research Area:
Neural Circuits
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, CRO Services
Cellular and molecular basis of leptin resistance

Bowen Tan, Kristina Hedbacker, Leah Kelly, Zhaoyue Zhang, Ji-Dung Luo, Joshua D. Rabinowitz, Jeffrey M. Friedman

Journal:
bioRxiv
Publication Date:
Research Area:
Metabolism
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, CRO Services
Xiphoid nucleus of the midline thalamus controls cold-induced food seeking

Neeraj K. Lal, Phuong Le, Samarth Aggarwal, Alan Zhang, Kristina Wang, Tianbo Qi, Zhengyuan Pang, Dong Yang, Victoria Nudell, Gene W. Yeo, Alexander S. Banks, Li Ye

Journal:
Nature
Publication Date:
Research Area:
Metabolism
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, CRO Services

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Further reading

LifeCanvas Portraits: Dr. Mike Taormina

Dr. Mike Taormina uses SmartSPIM light sheet microscopy to rapidly image whole mouse brains, streamlining workflows at the Allen Institute.