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Whole organ preservation, active clearing and labeling, and light sheet imaging have solved many of the issues related to thin-section histology, including high consumption of time and resources, spotlight bias, and realignment errors. LifeCanvas’s tissue processing pipeline is the world’s only end-to-end solution for processing whole organs from preservation to analysis. Download the white paper to learn more!

Blog Posts

Annotated altas image, Park et al 2018

Three easy steps to superior tissue preservation with SHIELD

In this blog, we will provide key insights in the first step of this journey: tissue preservation using SHIELD. SHIELD is a tissue-gel hybridization method using polyfunctional, flexible epoxides to preserve tissue architecture, endogenous fluorescence, protein antigenicity, and nucleic acids.

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Cleared lung tissue allowing visualization of the collagen structure and matrix of lung tissue at 20x magnification. Bharat et al., 2021.

How 3D tissue clearing benefits COVID-19 research

Over 190 million people have now been infected with the novel coronavirus SARS-CoV-2 in the ongoing coronavirus disease (COVID-19) pandemic with a total number of deaths passing 4.1 million worldwide. Multiple organs are affected by COVID-19, however, the lungs are the main site of disease.

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Mouse brain viral expression

Recent publications leverage 3D histology to provide new insights into neuronal circuitry

The goal of histology has long been to interrogate complex structures in whole tissue samples. Over centuries, slicing of samples for traditional two-dimensional histology has been indispensable in understanding the structure and morphology of multiple tissue types. However, this approach is limited to yielding images in thin tissue sections, is time-consuming and is error-prone due to tissue distortion and information loss.

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Accuracy of training data by number of users making annotations, for cell detection algorithm

How to create accurate training data for cell classification algorithms

Creating high-quality training data is a critical step when designing algorithms to detect and classify cells. Training data are example data points that are used to develop an algorithm. For example: a set of images, each of which might or might not contain a cell, plus human-generated tags indicating whether each image actually contains a cell.

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Mouse spinal cord expressing tdTomato, imaged with SmartSPIM

Upcoming publications to watch

Check out these upcoming publications utilizing LifeCanvas products and technologies! Our tissue processing methods and devices are utilized around the world to further medical and biological research, facilitating new discoveries and improving lives. The articles below are posted prior to peer-review in open-access medRxiv and bioRxiv online repositories.

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SDS monomers rupture cell membrane and form micelles to facilitate tissue clearing

How do detergents dissolve lipid membranes?

As explained in several of our blogs, delipidation is a critical step in the tissue clearing process. Some form of lipid removal is present in almost all of the most popular clearing techniques, including CLARITY, iDISCO, SHIELD, and CUBIC. In the case of SHIELD and our technologies, the detergent Sodium Dodecyl Sulfate (SDS) is used to dissolve the lipids

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Schematic of Zsigmondy's ultramicroscope

Our favorite inventions for National Inventor’s Month

April is National Inventor’s Month, and we wanted to give a shoutout to historical inventors that had a hand in creating the technologies we use every day in our manufacturing and tissue processing lab. Advances in science are incredibly interconnected, requiring the use of materials

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SHIELD preservation and clearing with SmartClear II Pro allows for good light penetration during imaging

Talking transparency: The history and physics of tissue clearing

There is a growing trend among biomedical researchers to interrogate biological structures and biomolecular information in three-dimensional volumes rather than in thin slices. This is particularly the case for neuroscientists looking to spatially map the interactions between the hundreds of unique cell types in the

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Neuropeptide Y staining in mouse brain dorsal cortex

3D labeling and imaging of Neuropeptide Y (NPY) localization in an intact mouse brain hemisphere

Neurons communicate not only via conventional neurotransmitters but also through neuropeptides. Whereas neurotransmitters often operate by changing the excitability of neighboring neurons, neuropeptides typically impact molecular pathways within target cells leading to diverse modulatory effects. Individual neurons can release both a conventional neurotransmitter and one

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New LifeCanvas tissue process pipeline

Tissue processing in the 21st century

Breathtaking progress in life sciences has brought us innovations such as high-throughput and individually-affordable genomic sequencing as well as next-generation flow cytometry that can phenotype dissociated cells for their expression of dozens of markers simultaneously. The development of cutting-edge technology solutions with standardized workflows has

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Why use intact samples for your research?

Histological analysis has classically been performed on thin tissue sections so that fine-scale features such as individual neuronal cell bodies and cellular processes can be resolved using light microscopy. This tissue-thickness limitation is due to two primary mechanisms: (#1) contamination of the focal plane by

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