
Immunohistochemistry (IHC) vs. 3D Histology
Immunohistochemistry (IHC) and 3D histology are different approaches to detecting specific proteins in biological tissue.
Immunohistochemistry (IHC) and 3D histology are different approaches to detecting specific proteins in biological tissue.
Dr. Sam Centanni uses the LifeCanvas pipeline to investigate the neurobiological overlap between stress and predisposition to alcohol abuse, as well as long-term effects of alcohol on the brain.
Dr. Wenqiang Chen leverages LifeCanvas whole brain mapping to study the interaction of insulin resistance with Alzheimer’s disease.
Different types of microtome are used to section different types of tissue, depending on the sample’s size, embedding medium, and preparation method.
Dr. Mike Taormina uses SmartSPIM light sheet microscopy to rapidly image whole mouse brains, streamlining workflows at the Allen Institute.
Light sheet fluorescence and laser scanning confocal microscopy are both used to acquire 3D image datasets with optical sectioning, however they differ significantly in imaging speed and resulting data quality.