Whole organ preservation, active clearing and labeling, and light sheet imaging have solved many of the issues related to thin-section histology, including high consumption of time and resources, spotlight bias, and realignment errors. LifeCanvas’s tissue processing pipeline is the world’s only end-to-end solution for processing whole organs from preservation to analysis. Download the white paper to learn more!
In this blog, we will provide key insights in the first step of this journey: tissue preservation using SHIELD. SHIELD is a tissue-gel hybridization method using polyfunctional, flexible epoxides to preserve tissue architecture, endogenous fluorescence, protein antigenicity, and nucleic acids.
Emerging organoid technologies have been used to model human organ development and diseases “in a dish”. Organoids are derived either from induced pluripotent or organ-restricted adult stem cells. These cells are self-renewing, self-organizing and able to grow into 3D structures resembling the architecture and physiology of the original biological specimens.
Over 190 million people have now been infected with the novel coronavirus SARS-CoV-2 in the ongoing coronavirus disease (COVID-19) pandemic with a total number of deaths passing 4.1 million worldwide. Multiple organs are affected by COVID-19, however, the lungs are the main site of disease.
The goal of histology has long been to interrogate complex structures in whole tissue samples. Over centuries, slicing of samples for traditional two-dimensional histology has been indispensable in understanding the structure and morphology of multiple tissue types. However, this approach is limited to yielding images in thin tissue sections, is time-consuming and is error-prone due to tissue distortion and information loss.
Creating high-quality training data is a critical step when designing algorithms to detect and classify cells. Training data are example data points that are used to develop an algorithm. For example: a set of images, each of which might or might not contain a cell, plus human-generated tags indicating whether each image actually contains a cell.
Check out these upcoming publications utilizing LifeCanvas products and technologies! Our tissue processing methods and devices are utilized around the world to further medical and biological research, facilitating new discoveries and improving lives. The articles below are posted prior to peer-review in open-access medRxiv and bioRxiv online repositories.
As explained in several of our blogs, delipidation is a critical step in the tissue clearing process. Some form of lipid removal is present in almost all of the most popular clearing techniques, including CLARITY, iDISCO, SHIELD, and CUBIC. In the case of SHIELD and our technologies, the detergent Sodium Dodecyl Sulfate (SDS) is used to dissolve the lipids
Although in the realm of neuroscience c-FOS protein is typically recognized as a marker of neuronal activity, it was originally discovered nearly forty years ago in rat fibroblasts and characterized as being encoded by a proto-oncogene (a gene that once mutated can become tumor-inducing) and
Recent advances in the tissue clearing field have unlocked the visualization of biology throughout whole organs. Tissue processing methods that render tissue optically transparent can be combined with methods that enable the tissue-wide penetration of molecular probes such as antibodies. However, many of these techniques
April is National Inventor’s Month, and we wanted to give a shoutout to historical inventors that had a hand in creating the technologies we use every day in our manufacturing and tissue processing lab. Advances in science are incredibly interconnected, requiring the use of materials
In 1906 at the University of Munich the neuropathologist Dr. Alois Alzheimer [Figure 1] was studying the brain of a recently deceased patient, Auguste Deter. Previously Alzheimer had assisted Frau Deter in the city asylum in Frankfurt and observed that she suffered from memory loss
Acetylcholine neurotransmission in the brain via the central cholinergic system has been implicated in critical functions including cognition, arousal, attention and learning. Moreover, interest in central cholinergic transmission has been piqued given that loss of cholinergic innervation may play an important role in neurodegenerative disorders
There is a growing trend among biomedical researchers to interrogate biological structures and biomolecular information in three-dimensional volumes rather than in thin slices. This is particularly the case for neuroscientists looking to spatially map the interactions between the hundreds of unique cell types in the
Neurons communicate not only via conventional neurotransmitters but also through neuropeptides. Whereas neurotransmitters often operate by changing the excitability of neighboring neurons, neuropeptides typically impact molecular pathways within target cells leading to diverse modulatory effects. Individual neurons can release both a conventional neurotransmitter and one
Prof. Li-Huei Tsai’s lab at MIT is focused on uncovering the cellular and circuit-level mechanisms that underlie Alzheimer’s disease, the devastating neurological disorder that degrades the brain’s learning and memory systems. In a 2016 paper in Nature (Iaccarino et al.), the lab reported the surprising
New to tissue clearing? If so, you are in the right place! This guide will walk you through the tissue processing journey step by step. First, to ensure the sample retains endogenous fluorescence, protein antigenicity, and structural integrity throughout the clearing process, the tissue
Breathtaking progress in life sciences has brought us innovations such as high-throughput and individually-affordable genomic sequencing as well as next-generation flow cytometry that can phenotype dissociated cells for their expression of dozens of markers simultaneously. The development of cutting-edge technology solutions with standardized workflows has
Histological analysis has classically been performed on thin tissue sections so that fine-scale features such as individual neuronal cell bodies and cellular processes can be resolved using light microscopy. This tissue-thickness limitation is due to two primary mechanisms: (#1) contamination of the focal plane by
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