c-FOS Brain Mapping: Challenges & Solutions

Mapping the localization of increased c-FOS expression provides important insights into specific brain regions implicated in the responses to a range of stimuli. Researchers have used c-FOS protein up-regulation as an indicator for a variety of cellular activities. For instance, this marker is a useful proxy for measuring the mammalian response to various stimuli including… Continue reading c-FOS Brain Mapping: Challenges & Solutions

Neurological disorders & 3D Histology

Microglia marked by IBA-1 (magenta) cluster around β-amyloid plaques (cyan) in brain of 7-month-old homozygous ARTE10 (disease model #16347) female mouse from Taconic Biosciences. SmartSPIM 15X objective.

Neuroscientists studying major neurological disorders such as Alzheimer’s, Parkinson’s, and Huntington’s diseases frequently use transgenic mouse models to evaluate disease progression and the impact of potential therapies. For instance, mice with overexpression of Alzheimer’s related proteins (e.g. amyloid precursor protein, APP) or mutations that trigger pathology (see Jankowsky and Zheng 2017) demonstrate the hallmarks of… Continue reading Neurological disorders & 3D Histology

LifeCanvas Portraits: Dr. Li Ye

Photo by Don Boomer

Updated April 13, 2022: Dr. Ye’s lab recently published “HYBRiD: hydrogel-reinforced DISCO for clearing mammalian bodies.” Learn more about the Ye Lab at the Scripps Research Institute. LifeCanvas (LC): How did you arrive at your current neuroscience research interests? Li Ye (LY): I have a background in peripheral metabolism, so I was interested in how… Continue reading LifeCanvas Portraits: Dr. Li Ye

3D Phenotyping with the SmartSPIM Light Sheet Microscope

After tissue clearing and (optional) labeling, intact tissue needs to be index-matched with EasyIndex refractive index-matching solution. Afterwards, tissues will appear fully transparent and ready for imaging using the SmartSPIM light sheet microscope. To complete your workflow, LifeCanvas Technologies offers SmartAnalytics – a powerful machine learning analysis tool, specially designed for large 3D datasets. This… Continue reading 3D Phenotyping with the SmartSPIM Light Sheet Microscope

LifeCanvas Portraits: Dr. Jose Maldonado

Jose Maldonado, Managing Director of Vanderbilt’s NeuroVisualization Lab, discusses his neuroscience research interests and how LifeCanvas’ imaging and analysis platforms helped quantify crucial data in his co-authored study published in Nature Communications this June.

3D labeling and imaging of Neuropeptide Y (NPY) localization in an intact mouse brain hemisphere

Figure 2: 400-µm MIP of Neuropeptide Y staining in mouse brain dorsal cortex.

Neurons communicate not only via conventional neurotransmitters but also through neuropeptides. Whereas neurotransmitters often operate by changing the excitability of neighboring neurons, neuropeptides typically impact molecular pathways within target cells leading to diverse modulatory effects. Individual neurons can release both a conventional neurotransmitter and one or more neuropeptides, providing the potential for multiple avenues of… Continue reading 3D labeling and imaging of Neuropeptide Y (NPY) localization in an intact mouse brain hemisphere

Whole brain molecular labeling & imaging techniques shed new light on Alzheimer’s Disease

Prof. Li-Huei Tsai’s lab at MIT is focused on uncovering the cellular and circuit-level mechanisms that underlie Alzheimer’s disease, the devastating neurological disorder that degrades the brain’s learning and memory systems. In a 2016 paper in Nature (Iaccarino et al.), the lab reported the surprising discovery that by simply entraining the activity of cortical networks… Continue reading Whole brain molecular labeling & imaging techniques shed new light on Alzheimer’s Disease