LifeCanvas Technologies is pioneering holistic intact sample tissue processing with its sample to data set pipeline and tools, seamlessly integrating each step in the workflow. As the only company to own the entire workflow from tissue preservation, clearing, antibody labeling, Refractive Index-matching, to SPIM imaging and analysis, we are positioned to break down barriers and empower researchers with our streamlined approaches to ensure the best results and data possible.
Seamless data acquisition requires getting the first step right. Our novel SHIELD (Park et al, Nature Biotech, 2019) tissue preservation technique forms intramolecular bonds using poyfunctional, flexible epoxides to stabilize tissue architecture and safeguard the sample's endogenous fluorescence, protein antigenicity and nucleic acids without the variability of hydrogel embedding.
By combining two powerful technologies - stochastic electrotransport (Kim et al, PNAS, 2015) and SWITCH (Murray et al, Cell, 2015), SmartLabel allows achieving whole-organ antibody labeling that is uniform from surface to core. Visualize proteins deep within the organ and study the fine-scale topography of the cells they identify.
After clearing and optional labeling, translucent, delipidated samples are incubated in EasyIndex raising and homogenizing their Refractive Index and turning them fully transparent. This increases light penetration into the sample and ensures that images will exhibit the highest resolution and focus. The cleared and labeled samples are then embedded in an agarose gel to stabilize them while imaging, guaranteeing alignment across channels and aiding colocalization of signals.
Built from the ground up, SmartSPIM is an advanced light sheet microscope capable of imaging large tissue samples with high speed and resolution. The system oﬀers superior imaging speed to two-photon and confocal while ensuring uniform axial resolution across the entire sample, generating data-sets with pixels sized 1.8 µm/px in XY and with 4 µm Z-steps (3.6x, 0.2 NA objective ~4.5 µm tall PSF). With rapid 4-color acquisition (488, 561, 642, & 785 laser lines) of your embedded samples, we can precisely overlay multiplexed immunoﬂuorescence signals and ﬂuorescent protein expression patterns alike.
New to tissue clearing? Don't worry - we have a versatile, simple and reliable passive clearing kit that includes everything you need! The bundle includes all the ready-made solutions to preserve, clear, and index match your samples with ease for 3-D imaging and analysis.
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