SHIELD reagent bottles

Tissue Preservation

Our novel SHIELD tissue preservation technique forms intramolecular bonds using polyfunctional, flexible epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids. SHIELD avoids the variability of hydrogel embedding and the information loss from PFA preservation, protecting specimens for multiple rounds of processing.

Tissue Clearing Buffers

Tissue Clearing & Labeling

Our new Clear+ tissue clearing technique is the only method that delipidates samples with no change in morphology and with minimal impact on structural integrity. Fully delipidate whole mouse brains or comparably sized samples in just one day with SmartBatch+, or in one week with our passive clearing kit.


Our turn-key SmartBatch+ system combines electrophoretic tissue clearing and immunolabeling into one high-throughput device. Leverage the Clear+ tissue clearing method, along with eFLASH and patented stochastic electrotransport technologies, to rapidly clear and label whole organs. Key highlights and features include:

  • Batch processing of up to 12 whole mouse brains, 4 whole rat brains, or comparably sized samples
  • Uniform labeling with no surface saturation of antibodies or dyes, and minimal antibody usage
  • No immunolabeling necessary for endogenous fluorescence (no fluorescent protein quenching)
  • 1-day active tissue clearing, 2-day active immunolabeling (primary & secondary)
EasyIndex bottle

Our preformulated EasyIndex solution raises and homogenize the refractive index of delipidated tissue samples, rendering them fully transparent. This enables light penetration into the sample and ensures the acquisition of high-resolution, in-focus image data.


Tissue Sectioning

Megatome is a vibrating microtome designed to section a broad range of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With high blade vibrating frequency and minimized blade deflection, Megatome enables high-throughput tissue sectioning with uniform surface profile, as well as minimal tissue damage and information loss. Additional features include mounting accessories, remote desktop control, and close-up cameras to facilitate the sectioning process.

Light Sheet Microscopy

The SmartSPIM light sheet microscope provides unparalleled resolution and acquisition speed throughout intact tissue volumes, while its modular design allows you to upgrade and customize individual components.
Key highlights and features include:

  • Patented axial sweeping technology, which generates homogenous PSFz across field of view
  • Rapid volumetric acquisition (intact mouse brain hemisphere in <30 minutes)
  • Large imaging chamber and dual illumination, optimized for large (up to 40mm x 75mm) or multi-sample acquisition – easily image organoids, rat brains, entire juvenile mice, & everything in between
  • Focus compensation and tunable imaging parameters
  • Intuitive acquisition software with real-time tile correction
  • Objectives available for 1.63X through 22X magnification

The MegaSPIM microscope is our newest light sheet imaging system. Utilizing the same powerful axial sweeping technology as SmartSPIM, MegaSPIM is optimized for imaging not only laterally large samples, but thin slices and arrays of small tissues as well. Unique highlights and features include:

  • Capacity for lateral sample sizes up to 200 mm x 200 mm –  easily image expanded tissues, large slabs, and arrays of organoids, slices, or whole rodent organs
  • Custom mounting solutions for a variety of sample types
  • High-throughput multicolor acquisition with dual cameras and independent focus correction
  • Post-processing tools to deskew and stitch your data into complete ready-to-analyze volumes
Heat map showing c-FOS+ fluorescence intensity by brain region in whole mouse brain

3D Data Analysis

Quantify your results using our SmartSPIM-optimized workstation. Perform cell detection across the entire sample using machine learning pipelines customized and optimized for 3D fluorescent microscopy images. Generate detailed analysis outputs: visualize your effects with our publication-quality heatmaps, and statistically verify your experimental questions with per-brain region cell counts and densities for each sample.