SHIELD

Exceptional tissue preservation

Published by Park et al. in Nature Biotechnology 2018, our SHIELD reagent (AKA GE38) safeguards endogenous fluorescence, protein antigenicity, nucleic acids, and overall tissue architecture against physical and chemical stressors. The SHIELD tissue preservation method also protects tissue during fluorescent protein imaging and antibody labeling across multiple rounds.

Compatible with:

All PFA-fixed tissues and multiplexing
SWITCH, MAP, SmartBatch+, and SmartLabel

Quick & easy protocol

Our streamlined SHIELD tissue preservation protocol takes just 4-6 days, without the variability of tissue embedding via hydrogel or paraffin. Reagents are non-toxic and can be easily disposed of after use.

Two kit sizes

250 mL: Preserves 12 whole mouse brains or comparably sized samples.

250 mL SHIELD Epoxy
125 mL SHIELD Buffer
250 mL SHIELD ON

500 mL: Preserves 25 whole mouse brains or comparably sized samples.

500 mL SHIELD Epoxy
250 mL SHIELD Buffer
500 mL SHIELD ON

Protocols

Documentation

Recent publications citing SHIELD

Bedenbaugh et al. “Organization of neural systems expressing melanocortin-3 receptors in the mouse brain: Evidence for sexual dimorphism.” Journal of Comparative Neurology (June 2022).
Schaffler et al. “A critical role for touch neurons in a skin-brain pathway for stress resilience.” bioRxiv (May 2022).
Yang et al. “Volumetric characterization of microvasculature in ex vivo human brain samples by serial sectioning optical coherence tomography.” IEEE Transactions on Biomedical Engineering (May 2022).
Roy et al. “Brain-wide mapping reveals that engrams for a single memory are distributed across multiple brain regions.” Nature Communications (April 2022).