SHIELD

Get the first step of tissue processing right with our SHIELD solution kit.

Exceptional protection of fluorescent proteins, tissue integrity, and endogenous molecular information.

VERSATILE

Safeguards endogenous fluorescence, protein antigenicity, nucleic acids, and overall tissue architecture against physical and chemical stressors. Synergizes with a range of existing tissue processing approaches including SWITCH (Cell, 2015), MAP (NBT, 2016), and SmartLabel.

EASY & RELIABLE

Superior tissue preservation, without the variability of hydrogel embedding. Compatible with all PFA-fixed tissues and multiplexing.

FAST & SAFE

Streamlined protocol preserves samples in a matter of days with minimal steps. Reagents are non-toxic and can be easily disposed of after use.

MULTI-ROUND PROTECTION

Protects tissue during fluorescent protein imaging and antibody labeling across multiple rounds.

Technical information:

250-mL SHIELD kit: Preserves 12 whole mouse brains or samples of equivalent size. Includes:

  • SHIELD Epoxy (250 mL)
  • SHIELD Buffer (125 mL)
  • SHIELD ON (250 mL)

500-mL SHIELD kit: Preserves 25 whole mouse brains or samples of equivalent size. Includes:

  • SHIELD Epoxy (500 mL)
  • SHIELD Buffer (250 mL)
  • SHIELD ON (500 mL)
 
GFP+ motor cortical neurons, imaged with SmartSPIM, courtesy of Prof. Byungkook Lim’s lab, UCSD.
RFP+ spinal cord cells, imaged with SmartSPIM, courtesy of Prof. Helen Lai’s lab, UT Southwestern.

Effective Workflow for PFA-Fixed Tissues