Versatile: Safeguards endogenous fluorescence, protein antigenicity, nucleic acids, and overall tissue architecture. Synergizes with a range of existing tissue processing approaches including SWITCH (Cell, 2015) and MAP (NBT, 2016). Other preservation techniques, such as PFA preservation alone, can lead to information loss due to physical or chemical stressors experienced during processing steps.
Easy & Reliable: Superior tissue preservation without the variability of hydrogel embedding. Compatible with both non-fixed and PFA-fixed tissues.
Fast: Streamlined protocol preserves samples in a matter of days with minimal steps.
Safe: Reagents are non-toxic and can be easily disposed of after use.
Repeated Staining & Imaging: Protects tissue during FP-imaging and antibody labeling across individual rounds.
250-mL SHIELD kit: Preserve 12 whole mouse brains or samples of equivalent size. Includes:
500-mL SHIELD kit: Preserve 25 whole mouse brains or samples of equivalent size. Includes:
Full Pipeline Protocol (includes SHIELD protocol)
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