SHIELD

Exceptional Protection of Fluorescent Proteins (FP) and Endogenous Molecular Information

Get the first step of tissue processing right with our easy & reliable solution kit

Superior Tissue Preservation

Versatile: Safeguards endogenous fluorescence, protein antigenicity, nucleic acids, and overall tissue architecture. Synergizes with a range of existing tissue processing approaches including SWITCH (Cell, 2015) and MAP (NBT, 2016). Other preservation techniques, such as PFA preservation alone, can lead to information loss due to physical or chemical stressors experienced during processing steps.

Easy, Fast, & Reliable

Easy & Reliable: Superior tissue preservation without the variability of hydrogel embedding. Compatible with both non-fixed and PFA-fixed tissues.

Fast: Streamlined protocol preserves samples in a matter of days with minimal steps.

Protects Tissue for Multi-Round Processing

Repeated Staining & Imaging: Protects tissue during FP-imaging and antibody labeling across individual rounds.

 SHIELD kit includes:
  • SHIELD Epoxy
  • SHIELD Buffer
  • SHIELD ON 

250-mL kit: Preserve 8 whole mouse brains or samples of equivalent size

500-mL kit: Preserve 16 whole mouse brains or samples of equivalent size

 
Figure from Park paper showing eGFP, YFP, and tdTomato preservation in mouse brain tissue using SHIELD
SHIELD workflow for fixing and clearing intact biological tissue
Bar graph demonstrating preservation of endogenous GFP signal with SHIELD compared to PFA
Figure from Park paper showing multi-round staining of mouse brain tissue