SHIELD

Exceptional tissue preservation

Published by Park et al. in Nature Biotechnology 2018, our SHIELD reagent (AKA GE38) safeguards endogenous fluorescence, protein antigenicity, nucleic acids, and overall tissue architecture against physical and chemical stressors. The SHIELD tissue preservation method also protects tissue during fluorescent protein imaging and antibody labeling across multiple rounds.

Compatible with:

Left: GFP+ motor cortical neurons, sample courtesy of Dr. Byungkook Lim, UCSD. Right: RFP+ spinal cord cells, sample courtesy of Dr. Helen Lai, UT Southwestern. Both imaged with SmartSPIM.

Quick & easy protocol

Our streamlined tissue preservation protocol takes just 4-6 days, without the variability of tissue embedding via hydrogel or paraffin. Reagents are non-toxic and can be easily disposed of after use.

Two kit sizes

250 mL: Preserves 12 whole mouse brains or comparably sized samples.

  • 250 mL SH-Epoxy
  • 125 mL SH-Buffer
  • 500 mL SH-ON

500 mL: Preserves 25 whole mouse brains or comparably sized samples.

  • 500 mL SH-Epoxy
  • 250 mL SH-Buffer
  • 1000 mL SH-ON
SHIELD2-100dpi

Recent publications citing SHIELD

Traumatic axonal injury in the optic nerve: the selective role of SARM1 in the evolution of distal axonopathy

Athanasios S. Alexandris, Youngrim Lee, Mohamed Lehar, Zahra Alam, James McKenney, Dianela Perdomo, Jiwon Ryu, Derek Welsbie, Donald J. Zack, Vassilis Koliatsos

Journal:
Journal of Neurotrauma
Publication Date:
Research Area:
Other
Tissue Species:
Mouse
Tissue Type:
Spinal Cord/Other Neuro
Products Cited:
SHIELD
Shared and Distinct Brain Regions Targeted for Immediate Early Gene Expression by Ketamine and Psilocybin

Pasha A. Davoudian, Ling-Xiao Shao, Alex C. Kwan

Journal:
ACS Chemical Neuroscience
Publication Date:
Research Area:
Neural Circuits
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, CRO Services
Whole-Brain Clearing and Immunolabelling in the African Killifish Nothobranchius furzeri

Augusto Ortega Granillo, Fengyan Deng, Hannah Wilson, Yongfu Wang, Alejandro Sánchez Alvarado

Journal:
Emerging Model Organisms
Publication Date:
Research Area:
Methods
Tissue Species:
Killifish
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartClear, SmartLabel

Further reading

Tissue processing in the 21st century

Immunohistochemistry methods are laborious, error-prone, and expensive – intact tissue processing technologies resolve many of these issues.