SHIELD

Tissue Preservation - Highlighted pipeline icon

Exceptional tissue preservation

Published by Park et al. in Nature Biotechnology 2018, our SHIELD reagent (AKA GE38) safeguards endogenous fluorescence, protein antigenicity, nucleic acids, and overall tissue architecture against physical and chemical stressors. The SHIELD tissue preservation method also protects tissue during fluorescent protein imaging and antibody labeling across multiple rounds.

Compatible with:

GFP+ motor cortical neurons in whole mouse brain (left) and tdTomato+ in a mouse spinal cord cell-type (right)
Left: GFP+ motor cortical neurons, sample courtesy of Dr. Byungkook Lim, UCSD. Right: RFP+ spinal cord cells, sample courtesy of Dr. Helen Lai, UT Southwestern. Both imaged with SmartSPIM.

Quick & easy tissue preservation protocol

Our streamlined protocol takes just 4-6 days, without the variability of tissue embedding via hydrogel or paraffin. Reagents are non-toxic and can be easily disposed of after use.

Figure showing SHIELD tissue preservation mechanism

Two SHIELD kit sizes

250 mL: Preserves 25 whole mouse brains or comparably sized samples.

  • 250 mL SH-Epoxy
  • 125 mL SH-Buffer
  • 500 mL SH-ON

500 mL: Preserves 50 whole mouse brains or comparably sized samples.

  • 500 mL SH-Epoxy
  • 250 mL SH-Buffer
  • 1000 mL SH-ON
SHIELD reagent bottles

Recent publications citing SHIELD

Astrocyte Gi-GPCR signaling corrects compulsive-like grooming and anxiety-related behaviors in Sapap3 knockout mice

Joselyn S. Soto, Chiranjivi Neupane, Muskan Kaur, Vijaya Pandey, James A. Wohlschlegel, Baljit S. Khakh

Journal:
Neuron
Publication Date:
Research Area:
cFos
Tissue Species:
Mouse
Products Cited:
SHIELD, SmartSPIM, SmartAnalytics, CRO Services
Experience-dependent information routing through the basolateral amygdala shapes behavioral outcomes

Pantelis Antonoudiou, Bradly T. Stone, Phillip L.W. Colmers, Aidan Evans-Strong, Eric Teboul, Najah L. Walton, Grant L. Weiss, Jamie Maguire.

Journal:
Cell Reports
Publication Date:
Research Area:
Neural Circuits
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, SmartSPIM
Serine racemase deletion alters adolescent social behavior and whole-brain cFos activation

Stephanie E. Brown, Ziyi (Zephyr) Wang, Emily L. Newman, Elif Engin, Sabina Berretta, Darrick T. Balu, Oluwarotimi O. Folorunso.

Journal:
Frontiers in Psychiatry
Publication Date:
Research Area:
cFos, Mapping
Tissue Species:
Mouse
Tissue Type:
Heart
Products Cited:
SHIELD, SmartBatch+, SmartSPIM, CRO Services

Further reading