SHIELD

Tissue Preservation - Highlighted pipeline icon

Exceptional tissue preservation

Published by Park et al. in Nature Biotechnology 2018, our SHIELD reagent (AKA GE38) safeguards endogenous fluorescence, protein antigenicity, nucleic acids, and overall tissue architecture against physical and chemical stressors. The SHIELD tissue preservation method also protects tissue during fluorescent protein imaging and antibody labeling across multiple rounds.

Compatible with:

GFP+ motor cortical neurons in whole mouse brain (left) and tdTomato+ in a mouse spinal cord cell-type (right)
Left: GFP+ motor cortical neurons, sample courtesy of Dr. Byungkook Lim, UCSD. Right: RFP+ spinal cord cells, sample courtesy of Dr. Helen Lai, UT Southwestern. Both imaged with SmartSPIM.

Quick & easy tissue preservation protocol

Our streamlined protocol takes just 4-6 days, without the variability of tissue embedding via hydrogel or paraffin. Reagents are non-toxic and can be easily disposed of after use.

Figure showing SHIELD tissue preservation mechanism

Two SHIELD kit sizes

250 mL: Preserves 25 whole mouse brains or comparably sized samples.

  • 250 mL SH-Epoxy
  • 125 mL SH-Buffer
  • 500 mL SH-ON

500 mL: Preserves 50 whole mouse brains or comparably sized samples.

  • 500 mL SH-Epoxy
  • 250 mL SH-Buffer
  • 1000 mL SH-ON
SHIELD reagent bottles

Recent publications citing SHIELD

A topographical atlas of α-synuclein cell type-specfic expression in adult mouse brain and peripheral organs

Maxime W C Rousseaux, Haley Geertsma, Zoe Fisk, Lillian Sauline, Alice Prigent, Kevin Kurgat, Steve Callaghan, Julianna Tomlinson, Michael Henderson

Journal:
Research Square
Publication Date:
Research Area:
Neurodegeneration
Tissue Species:
Mouse
Tissue Type:
Brain, Spinal Cord, GI Tract
Products Cited:
SHIELD, CRO Services
Mapping oto-pharyngeal development in a human inner ear organoid model

Matthew R. Steinhart, Wouter H. van der Valk, Daniel Osorio, Sara A. Serdy, Jingyuan Zhang, Carl Nist-Lund, Jin Kim, Cynthia Moncada-Reid, Liang Sun, Jiyoon Lee, Karl R. Koehler

Journal:
Development
Publication Date:
Research Area:
Stem Cells
Tissue Species:
Human
Tissue Type:
Organoid
Products Cited:
SHIELD, EasyIndex
A Light sheet fluorescence microscopy and machine learning-based approach to investigate drug and biomarker distribution in whole organs and tumors

Niyanta Kumar, Petr Hrobar, Martin Vagenknecht, Jindrich Soukup, Nadia Patterson, Peter Bloomingdale, Tomoko Freshwater, Sophia Bardehle, Roman Peter, Ruban Mangadu, Cinthia V Pastuskovas, Chiswili Yves Chabu, Mark T. Cancilla

Journal:
bioRxiv
Publication Date:
Research Area:
Neurodegeneration, Cancer, Vasculature, Methods
Tissue Species:
Mouse
Tissue Type:
Brain, Tumor
Products Cited:
SHIELD, EasyIndex, SmartClear, SmartLabel, SmartSPIM, SmartAnalytics, CRO Services

Further reading

Tissue processing in the 21st century

Immunohistochemistry methods are laborious, error-prone, and expensive – intact tissue processing technologies resolve many of these issues.