SHIELD

Exceptional tissue preservation

Published by Park et al. in Nature Biotechnology 2018, our SHIELD reagent (AKA GE38) safeguards endogenous fluorescence, protein antigenicity, nucleic acids, and overall tissue architecture against physical and chemical stressors. The SHIELD tissue preservation method also protects tissue during fluorescent protein imaging and antibody labeling across multiple rounds.

Compatible with:

Left: GFP+ motor cortical neurons, sample courtesy of Dr. Byungkook Lim, UCSD. Right: RFP+ spinal cord cells, sample courtesy of Dr. Helen Lai, UT Southwestern. Both imaged with SmartSPIM.

Quick & easy protocol

Our streamlined tissue preservation protocol takes just 4-6 days, without the variability of tissue embedding via hydrogel or paraffin. Reagents are non-toxic and can be easily disposed of after use.

Two kit sizes

250 mL: Preserves 12 whole mouse brains or comparably sized samples.

  • 250 mL SH-Epoxy
  • 125 mL SH-Buffer
  • 500 mL SH-ON

500 mL: Preserves 25 whole mouse brains or comparably sized samples.

  • 500 mL SH-Epoxy
  • 250 mL SH-Buffer
  • 1000 mL SH-ON
SHIELD2-100dpi

Further reading

Tissue processing in the 21st century

Breathtaking progress in life sciences has brought us innovations such as high-throughput and individually-affordable genomic sequencing as well as next-generation...