SHIELD

Exceptional tissue preservation

Published by Park et al. in Nature Biotechnology 2018, our SHIELD reagent safeguards endogenous fluorescence, protein antigenicity, nucleic acids, and overall tissue architecture against physical and chemical stressors. The SHIELD method also protects tissue during fluorescent protein imaging and antibody labeling across multiple rounds.

Compatible with:

All PFA-fixed tissues and multiplexing
SWITCH, MAP, SmartBatch+, and SmartLabel

Quick & easy protocol

Our streamlined SHIELD protocol preserves samples in 4-6 days without the variability of tissue embedding via hydrogel or paraffin. Reagents are non-toxic and can be easily disposed of after use.

Two kit sizes

250 mL: Preserves 12 whole mouse brains or comparably sized samples.

250 mL SHIELD Epoxy
125 mL SHIELD Buffer
250 mL SHIELD ON

500 mL: Preserves 25 whole mouse brains or comparably sized samples.

500 mL SHIELD Epoxy
250 mL SHIELD Buffer
500 mL SHIELD ON

Protocols

Documentation

Publications citing SHIELD

2021:

Nobre et al. “MiRNA-Mediated Knockdown of ATXN3 Alleviates Molecular Disease Hallmarks in a Mouse Model for Spinocerebellar Ataxia Type 3.” Nucleic Acid Therapeutics (December 2021).
Menzel et al. “Lymphocyte access to lymphoma is impaired by high endothelial venule regression.” Cell Reports (October 2021).
Foster et al. “The Mouse Cortico-Basal Ganglia-Thalamic Network.” Nature (October 2021).
Muñoz-Castañeda et al. “Cellular Anatomy of the Mouse Primary Motor Cortex.” Nature (October 2021).
Benavidez et al. “Organization of the inputs and outputs of the mouse superior colliculus.” Nature Communications (June 2021).
Luchsinger et al. “Delineation of an insula-BNST circuit engaged by struggling behavior that regulates avoidance in mice.” Nature Communications (June 2021).
Hintiryan et al. “Connectivity characterization of the mouse basolateral amygdalar complex.” Nature Communications (May 2021).
Helseth et al. “Cholinergic neurons constitutively engage the ISR fordopamine modulation and skill learning in mice.” Science (April 2021).
Sweeney et al. “The melanocortin-3 receptor is a pharmacological target for the regulation of anorexia.” Science Translational Medicine (April 2021).
Bharat et al. “Early Outcomes After Lung Transplantation for Severe COVID-19: A Series of the First Consecutive Cases from Four Countries.” The Lancet Respiratory Medicine (March 2021).

2020:

Albanese et al. “Multiscale 3D Phenotyping of Human Cerebral Organoids.” Scientific Reports (December 2020).
Bharat et al. “Lung Transplantation for Patients with Severe COVID-19.” Science Translational Medicine (November 2020).
Pop et al. “Evidence for Genetically Distinct Direct and Indirect Spinocerebellar Pathways Mediating Proprioception.” bioRxiv (August 2020).
Klinghoffer et al. “Self-Supervised Feature Extraction for 3D Axon Segmentation.” arXiv (April 2020).
Ogujiofor et al. “Motor Neurons Involved in Fine Motor Control Are Labeled by Tracing Atoh1-Lineage Neurons in the Spinal Cord.” bioRxiv (March 2020).
Benavidez et al. “The Mouse Cortico-Tectal Projectome.” bioRxiv (March 2020).

2019:

Roy et al. “Brain-Wide Mapping of Contextual Fear Memory Engram Ensembles Supports the Dispersed Engram Complex Hypothesis.” bioRxiv (June 2019).
Yun et al. “Ultrafast Immunostaining of Organ-Scale Tissues for Scalable Proteomic Phenotyping.” bioRxiv (June 2019).
Martorell et al. “Multi-sensory Gamma Stimulation Ameliorates Alzheimer’s-Associated Pathology and Improves Cognition.” Cell (April 2019).
Swaney et al. “Scalable Image Processing Techniques for Quantitative Analysis of Volumetric Biological Images from Light-Sheet Microscopy.” bioRxiv (March 2019).

2018:

Park et al. “Protection of Tissue Physicochemical Properties Using Polyfunctional Crosslinkers.” Nature Biotechnology (December 2018).