SmartLabel
The Ultimate Active Immunostaining Device
The Ultimate Active Immunostaining Device
Email us at labeling@lifecanvastech.com for more information.
Why Early Adopter?
- Gain a competitive edge in whole-organ labeling and receive individualized technical support designed to get you generating data quickly.
- Exchange tissue samples with LifeCanvas scientists at various stages of the preservation, clearing, labeling, & imaging pipeline to get feedback on your preparation skills and master the entire workflow easily.
- Exclusive opportunity to have LifeCanvas validate new antibodies or labeling reagents of your choice for your specific tissue type & application.
Rapid Immunostaining
with Unparalleled Uniformity
Fast, Easy, and Efficient
Fast, Easy, and Efficient
Fast: Label large, intact samples such as rodent organs an order of magnitude faster than with passive labeling (≤24 hours vs. weeks to months).
Easy: Turnkey operation – simply load the buffers and your sample & probes. Perform 2 distinct labeling experiments simultaneously thanks to SmartLabel’s dual chamber design.
Efficient: Uses a small amount of antibody (as little as ~3 µg) to label tissues the size of a whole mouse brain.
Reliable: Prevents tissue damage with Stochastic Electrotransport technology (Kim, PNAS, 2015). Our nanoporous membranes (patent-pending) eliminate tissue contamination and probe loss.
Complete and Uniform Labeling
Complete and Uniform Labeling
- Achieves unparalleled uniformity of signal intensity from surface to core using LifeCanvas’s advanced eFLASH (Yun et al.. 2019) protocol.
- Combines rapid, Stochastic Electrotransport-mediated (Kim et al., 2015) infiltration of probes into tissue with the SWITCH technique (Murray et al., 2015) of controlling probe binding kinetics.
Cost Savings by Avoiding Target Saturation
Cost Savings by Avoiding Target Saturation
- Prevents antibody depletion caused by excessive binding to superficial tissue sites and ensures the sample’s center is well-labeled.
Compatible with Many Cleared-Tissue Techniques
Compatible with Many Cleared-Tissue Techniques
Anti-GFAP Staining in CA1 Hippocampus of 2-mm Thick Section of Mouse Brain
Volume rendering of anti-GFAP staining in a 2-mm thick section of mouse brain, with signal in CA1 hippocampus most prominent. Labeling achieved in 2 days using antibody electrophoresis performed by SmartLabel. Cleared with SmartClear II Pro (<1 day), refractive index-matched with EasyIndex, and light-sheet imaged with SmartSPIM (<4 minutes acquisition time with 2-µm z-step and 1.8 µm/pixel XY; 2×2 tile using a 3.6x, 0.2 NA objective).
SmartLabel: Rapid Immunostaining with Unparalleled Uniformity
SmartLabel, the world’s first active labeling device, combines SWITCH and stochastic electrotransport technologies to quickly and uniformly label whole tissue samples in a fraction of the time needed for passive labeling. Stain an entire mouse brain or rat hemisphere in 24 hours or less and avoid the time-intensive, destructive sectioning and tedious image reconstruction of traditional histology.
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