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Fast: Label large, intact samples such as rodent organs an order of magnitude faster than with passive labeling (≤ 24 hours vs. weeks to months).
Easy: Turnkey operation – simply load the buffers and your sample & probes. Perform 2 distinct labeling experiments simultaneously thanks to SmartLabel’s dual chamber design.
Efficient: Uses a small amount of antibody (as little as ~3 µg) to label tissues the size of a whole mouse brain.
Reliable: Prevents tissue damage with Stochastic Electrotransport technology (Kim, PNAS, 2015). Our nanoporous membranes (patent-pending) eliminate tissue contamination and probe loss.
Volume rendering of anti-GFAP staining in a 2-mm thick section of mouse brain, with signal in CA1 hippocampus most prominent. Labeling achieved in 2 days using antibody electrophoresis performed by SmartLabel. Cleared with SmartClear II Pro (<1 day), refractive index-matched with EasyIndex, and light-sheet imaged with SmartSPIM (<4 minutes acquisition time with 2-µm z-step and 1.8 µm/pixel XY; 2×2 tile using a 3.6x, 0.2 NA objective).
SmartLabel, the world’s first active labeling device, combines SWITCH and stochastic electrotransport technologies to quickly and uniformly label whole tissue samples in a fraction of the time needed for passive labeling. Stain an entire mouse brain or rat hemisphere in 24 hours or less and avoid the time-intensive, destructive sectioning and tedious image reconstruction of traditional histology.
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