SmartLabel

The Ultimate Active Immunostaining Device

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Quick Installation Guide

Device Setup

Rapid Immunostaining
with Unparalleled Uniformity

Fast, Easy, and Efficient

Fast: Label large, intact samples such as rodent organs an order of magnitude faster than with passive labeling (≤ 24 hours vs. weeks to months).

Easy: Turnkey operation – simply load the buffers and your sample & probes. Perform 2 distinct labeling experiments simultaneously thanks to SmartLabel’s dual chamber design.

Efficient: Uses a small amount of antibody (as little as ~3 µg) to label tissues the size of a whole mouse brain.

Reliable: Prevents tissue damage with Stochastic Electrotransport technology (Kim, PNAS, 2015). Our nanoporous membranes (patent-pending) eliminate tissue contamination and probe loss.

Complete and Uniform Labeling

Achieves unparalleled uniformity of signal intensity from surface to core using LifeCanvas’s advanced eFLASH (Yun, bioRxiv, 2019) protocol.
Combines rapid, Stochastic Electrotransport-mediated (Kim, PNAS, 2015) infiltration of probes into tissue with the SWITCH technique (Murray, Cell, 2015) of controlling probe binding kinetics.

Cost Savings by Avoiding Target Saturation

Prevents antibody depletion caused by excessive binding to superficial tissue sites and ensures the sample’s center is well-labeled.

Compatible with Many Cleared-Tissue Techniques

SHIELD (Park, Nature Biotechnology, 2018)
MAP (Ku, Nature Biotechnology, 2016)
SWITCH (Murray, Cell, 2015)

GFAP (cyan) and Beta-amyloid (magenta) staining in mouse brain imaged at 15x — Beta Amyloid plaques (magenta) and astrocytes (cyan) in mouse brain model of Alzheimer's Disease imaged at 15x with SmartSPIM.

Tyrosine hydroxylase staining in mouse brain hemisphere — Tyrosine Hydroxylase staining in a mouse brain hemisphere. Labeled with SmartLabel using only 3 µg primary antibody.

Lectin (red) and Olf4 (cyan) immunolabeling of intact mouse intestine — Injected lectin conjugate (red) and Olf4 (cyan) stem cell marker in a mouse duodenum segment.

Parvalbumin staining in mouse cerebellum imaged at 3.6x — Rapid, one-step immunolabeling of a subset of inhibitory neurons in a mouse brain hemisphere using SmartLabel. Image shows a 3D rendering of the cerebellum.

SYTO16 + Lectin in mouse brain hemi — SYTO16 (cyan) nuclear stain and lectin (magenta) vascular stain highlight tumor morphology in a mouse brain hemisphere harboring a xenografted striatal tumor.

MBP staining in mouse cerebellum — Uniform anti-Myelin Basic Protein labeling in a mouse cerebellum.

Phospho-tau staining of human frontal cortex — Slab of human frontal cortex tissue labeled using SmartLabel with anti-Phospho-Tau, a hallmark of Alzheimer's disease.

Anti-GFAP Staining in CA1 Hippocampus of 2-mm Thick Section of Mouse Brain

Volume rendering of anti-GFAP staining in a 2-mm thick section of mouse brain, with signal in CA1 hippocampus most prominent. Labeling achieved in 2 days using antibody electrophoresis performed by SmartLabel. Cleared with SmartClear II Pro (<1 day), refractive index-matched with EasyIndex, and light-sheet imaged with SmartSPIM (<4 minutes acquisition time with 2-µm z-step and 1.8 µm/pixel XY; 2×2 tile using a 3.6x, 0.2 NA objective).

SmartLabel: Rapid Immunostaining with Unparalleled Uniformity

SmartLabel, the world’s first active labeling device, combines SWITCH and stochastic electrotransport technologies to quickly and uniformly label whole tissue samples in a fraction of the time needed for passive labeling. Stain an entire mouse brain or rat hemisphere in 24 hours or less and avoid the time-intensive, destructive sectioning and tedious image reconstruction of traditional histology.