Tissue Clearing Services

Customized intact tissue clearing

LifeCanvas can clear and image whole organs with endogenous fluorescent reporters (e.g. GFP, YFP, RFPs) tagging select cells and processes. High-throughput, electrophoretic tissue clearing with SmartBatch+ ensures preservation of fluorescent signal, sample integrity, and experimental consistency.

Right: GFP+ motor cortical neurons projecting to striatum via rabies virus expressing eGFP. Sample courtesy of Dr. Byungkook Lim, UCSD.

Full service tissue processing

Upon receiving your samples, LifeCanvas technicians:

  • Prepare them with SHIELD to preserve tissue integrity and endogenous fluorescence
  • Actively clear them using SmartBatch+
  • Index-match with EasyIndex
  • Acquire volumetric light sheet images with SmartSPIM
  • Analyze post-processed images with SmartAnalytics to derive cell counts, densities, heat maps, and graphical outputs

From tissue samples to publishable data

You send us: PFA-fixed samples from your experimental animals

Based on your needs, we can provide:

  • Mapping of endogenous fluorescent reporters throughout tissue volumes
  • Full processing and imaging for diverse tissue types, including: spinal cord, kidney, liver, heart, lung, intestine, organoids, tumors

Endogenous Thy1-driven YFP expression in an adult mouse brain. Sample courtesy of Dr. G. Allan Johnson, Duke Center for In Vivo Microscopy.

Speak to an expert about your application

Further reading

Header: tdTomato labeling of a mouse spinal cord cell-type. Sample courtesy of Dr. Helen Lai, UT Southwestern.