SeTau-647 Secondary Antibody

Ensuring superior photostability when labeling

Our SeTau-647 Secondary Antibody is the only commercially available pre-conjugated SeTau Secondary that will provide superior photostability when labeling. 

Features and highlights:

  • Most photostable far-red fluorescent dye we have tested.
  • Fast, uniform labeling: Combine with SmartBatch+ for the best results when staining with antibodies in the 647 channel. 
  • Applications: Ideal for light sheet, 2P Imaging, expansion tissues, super-resolution, or live cell imaging where fluorescence stability is paramount.
  • SeTau-647 secondary antibody is available in 0.5 mg vials. Each vial is preconjugated with SeTau-647 from SETA Biomedicals.

Video shows how SeTau-647 maintains photostability over time compared to a conventional 647 fluorophore. A whole mouse brain was labeled with anti-NeuN primary antibody. After hemisecting the brain, the hemisphere on the left was stained with a conventional 647 fluorophore secondary antibody, and the hemisphere on the right with SeTau-647. Hemispheres were glued together and imaged on SmartSPIM at 1.6X with 100% laser power.

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SeTau-647 versus a conventional fluorophore

Photobleaching occurs when a fluorophore loses its fluorescence due to light-induced damage. As different fluorophores have varied susceptibility to this phenomenon, we measure fluorescence intensity of different fluorophores over time to determine photostability.

LifeCanvas developed SeTau-647 Secondary Antibody which as observed in the graph, shows higher fluorescence intensity sustainability over time compared to conventional 647 fluorophores. This establishes SeTau-647 as the premier choice, showcasing superior photostability over conventional alternatives.

Minimizing photobleaching is crucial for light sheet imaging and other applications requiring fluorescence stability, such as 2P, expansion tissues, super-resolution, and live cell imaging.

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Documentation

Protocols

Recent publications citing SmartBatch+

Serine racemase deletion alters adolescent social behavior and whole-brain cFos activation

Stephanie E. Brown, Ziyi (Zephyr) Wang, Emily L. Newman, Elif Engin, Sabina Berretta, Darrick T. Balu, Oluwarotimi O. Folorunso.

Journal:
Frontiers in Psychiatry
Publication Date:
Research Area:
cFos, Mapping
Tissue Species:
Mouse
Tissue Type:
Heart
Products Cited:
SHIELD, SmartBatch+, SmartSPIM, CRO Services
A cocaine-activated ensemble exerts increased control over behavior while decreasing in size

Kimberly C. Thibeault, Michael Z. Leonard, Veronika Kondev, Soren D. Emerson, Rishik Bethi, Alberto J. Lopez, Jonathon P. Sens, Brett P. Nabit, Hannah B. Elam, Danny G. Winder, Sachin Patel, Drew D. Kiraly, Brad A. Grueter, Erin S. Calipari.

Journal:
Biological Psychiatry
Publication Date:
Research Area:
cFos, Mapping
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, SmartBatch+, SmartSPIM, CRO Services
The functional and anatomical characterization of three spinal output pathways of the anterolateral tract

Haichao Chen, Isabel H. Bleimeister, Eileen K. Nguyen, Jie Li, Abby Yilin Cui, Harrison J. Stratton, Kelly M. Smith, Mark L. Baccei, Sarah E. Ross.

Journal:
Cell Reports
Publication Date:
Research Area:
Neural Circuits, Mapping
Tissue Species:
Mouse
Tissue Type:
Spinal Cord
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, CRO Services
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Further reading

LifeCanvas Portraits: Dr. Mike Taormina

Dr. Mike Taormina uses SmartSPIM light sheet microscopy to rapidly image whole mouse brains, streamlining workflows at the Allen Institute.

For technical support reach out to our dedicated customer support team at support@lifecanvastech.com.