SeTau-647 Secondary Antibody

Ensuring superior photostability when labeling

Our SeTau-647 Secondary Antibody is the only commercially available pre-conjugated SeTau Secondary that will provide superior photostability when labeling. 

Features and highlights:

  • Most photostable far-red fluorescent dye we have tested.
  • Fast, uniform labeling: Combine with SmartBatch+ for the best results when staining with antibodies in the 647 channel. 
  • Applications: Ideal for light sheet, 2P Imaging, expansion tissues, super-resolution, or live cell imaging where fluorescence stability is paramount.
  • SeTau-647 secondary antibody is available in 0.5 mg vials. Each vial is preconjugated with SeTau-647 from SETA Biomedicals.

Video shows how SeTau-647 maintains photostability over time compared to a conventional 647 fluorophore. A whole mouse brain was labeled with anti-NeuN primary antibody. After hemisecting the brain, the hemisphere on the left was stained with a conventional 647 fluorophore secondary antibody, and the hemisphere on the right with SeTau-647. Hemispheres were glued together and imaged on SmartSPIM at 1.6X with 100% laser power.

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SeTau-647 versus a conventional fluorophore

Photobleaching occurs when a fluorophore loses its fluorescence due to light-induced damage. As different fluorophores have varied susceptibility to this phenomenon, we measure fluorescence intensity of different fluorophores over time to determine photostability.

LifeCanvas developed SeTau-647 Secondary Antibody which as observed in the graph, shows higher fluorescence intensity sustainability over time compared to conventional 647 fluorophores. This establishes SeTau-647 as the premier choice, showcasing superior photostability over conventional alternatives.

Minimizing photobleaching is crucial for light sheet imaging and other applications requiring fluorescence stability, such as 2P, expansion tissues, super-resolution, and live cell imaging.

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Documentation

Protocols

Recent publications citing SmartBatch+

Phosphorylation of pyruvate dehydrogenase inversely associates with neuronal activity.

Dong Yang, Yu Wang, Tianbo Qi, Xi Zhang, Leyao Shen, Jingrui Ma, Zhengyuan Pang, Neeraj K. Lal, Daniel B. McClatchy, Saba Heydari Seradj, Verina H. Leung, Kristina Wang, Yi Xie, Filip S. Polli, Anton Maximov, Oscar Christian Gonzalez, Luis de Lecea, Hollis T. Cline, Vineet Augustine, John R. Yates III, and Li Ye.

Journal:
Neuron
Publication Date:
Research Area:
Neural Circuits, Neural Interfaces
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, SmartAnalytics, CRO Services
Drugs of abuse hijack a mesolimbic pathway that processes homeostatic need

Bowen Tan, Caleb J. Browne, Tobias Nöbauer, Alipasha Vaziri, Jeffrey M. Friedman, Eric J. Nestler

Journal:
bioRxiv
Publication Date:
Research Area:
Neural Circuits
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, CRO Services
Cellular and molecular basis of leptin resistance

Bowen Tan, Kristina Hedbacker, Leah Kelly, Zhaoyue Zhang, Ji-Dung Luo, Joshua D. Rabinowitz, Jeffrey M. Friedman

Journal:
bioRxiv
Publication Date:
Research Area:
Metabolism
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, CRO Services
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Further reading

LifeCanvas Portraits: Dr. Mike Taormina

Dr. Mike Taormina uses SmartSPIM light sheet microscopy to rapidly image whole mouse brains, streamlining workflows at the Allen Institute.

For technical support reach out to our dedicated customer support team at support@lifecanvastech.com.