c-FOS expression is an indicator of recent neural cell activity, making the detection of c-FOS+ cells a high priority for many neuroscientists. This image stack captures the hippocampus and cortex in the transverse plane of an intact mouse brain stained with anti-c-FOS. These images were acquired with SmartSPIM at 3.6X magnification and 4 µm z-step.
Ultimately, quantification is key. To complete the end-to-end tissue processing pipeline, LifeCanvas has developed SmartAnalytics, a powerful machine learning analysis tool designed for large 3D datasets. SmartAnalytics currently offers:
Left: Heatmap of cell density by region in mouse brain hemisphere stained with anti-c-FOS, generated with SmartAnalytics. Right: Histogram of cell density by region in same c-FOS stained hemisphere, generated by SmartAnalytics.
Right: An image stack captures the hippocampus and cortex in the sagittal plane of a mouse brain left hemisphere stained with anti-c-FOS. Images acquired with SmartSPIM at 3.6X magnification and 4 µm z-step. Scale bar 500 µm.