Services

Whole Organ Mapping

LifeCanvas provides exceptional-quality whole organ mapping services to meet and surpass your research goals. Our team has extensive expertise in tissue processing and imaging, as well as robust image analysis methods for complex, information-rich datasets. Just send us your PFA-fixed samples, and we’ll do the rest.

Header: Subpopulation of parvalbumin (PV) -expressing GABAergic neurons visible throughout mouse hemisphere labelled with anti-PV, 3.6X. Left: Mouse spinal cord labeled with tdTomato. Sample courtesy of Lai Lab, UT Southwestern. Scale bar = 300 µm.

Leverage our comprehensive pipeline

Figure 1. SHIELD cross-links biological samples.
Tissue Preservation
Delipidation
Delipidation
Immunolabeling
Immunolabeling
Clearing
Optical Clearing
Imaging
Light Sheet Imaging
analysis
3D Image Analysis

Featured Applications

We can clear and image intact samples with endogenous fluorescent reporters (e.g. GFP, YFP, RFPs) tagging select cells and processes. Our tools preserve fluorescent proteins exceptionally well, and work with a variety of tissue and sample types, including: spinal cord, kidney, liver, heart, lung, intestine, organoids, and tumors.

Right: Thy1-driven YFP expression in an adult mouse brain. Sample courtesy of G. Allan Johnson, Duke Center for In Vivo Microscopy.

We have perfected technology to label and image c-FOS+ cells throughout the CNS. Using our robust models for detecting c-FOS+ cells in mouse brains, we provide: cell counts, densities, and heat maps quantifying levels of regional neural activity registered to the Allen Brain Atlas.

Left: Hippocampus and cortex in sagittal plane of mouse brain left hemisphere stained with anti-c-FOS (white), with detected cells in red. 3.6X, scale bar = 500 µm.

LifeCanvas can process and analyze samples for β-amyloid deposition in Alzheimer’s disease models, and for accumulation of α-synuclein in Lewy Bodies in Parkinson’s disease models. We determine levels of protein aggregation for regionally specific brain areas registered to the Allen Brain Atlas.

Left: GFAP (cyan) and Beta-amyloid plaques (magenta) in 5xFAD mouse brain model of Alzheimer’s disease. 15X, scale bar = 100 µm. Sample courtesy of Epp Lab, University of Calgary.

Our versatile technologies allow us to label multiple targets, including specific cell types (e.g. neurons, glia), nuclei, and vasculature. We can also process multiple organ or sample types using tissue-specific markers.

Left: Mouse duodenum segment, labeled with lectin (red, vasculature) and anti-Olfm4 (cyan, stem cell marker). Sample courtesy of Dr. Chaudhry, Ferrara Bone Marrow Transplantation Lab, Icahn School of Medicine.