Services

Sample-to-result whole organ mapping

LifeCanvas provides exceptional-quality whole organ mapping services to meet and surpass your research goals. Our team has extensive expertise in tissue processing and imaging, as well as robust image analysis methods for complex, information-rich datasets. Just send us your PFA-fixed samples, and we’ll do the rest.

Left: Mouse spinal cord labeled with tdTomato. Sample courtesy of Dr. Helen Lai, UT Southwestern. Scale bar = 300 µm.

Leverage our comprehensive pipeline

Tissue Preservation
Delipidation
Immunolabeling
Optical Clearing
Light Sheet Imaging
3D Image Analysis

Interactive, high-quality data delivery

Our interactive data delivery platform presents your data in an easily digestible and customizable format. View the experimental effect on multiple brain regions in side-by-side bar plots, and compare treatment groups with subtracted heatmaps.

We deliver:

  • Regional cell counts registered to the Allen Brain Atlas
  • Heat maps showing localized levels of protein expression
  • Coronal views with Atlas overlays depicting labeled cells throughout the whole brain volume

Expertise in diverse sample species and types

  • Whole rodent juvenile
  • Whole rodent head
  • Brain
  • Peroneal nerve
  • Sciatic nerve
  • Dorsal root ganglion
  • Spinal cord
  • Heart
  • Lung
  • Liver
  • Pancreas
  • Kidney
  • Organoid
  • Adipose tissue
  • Skin
  • Eye
  • Ear
  • Muscle
  • Limb
  • Placenta

Featured Applications

We can clear and image intact samples with endogenous fluorescent reporters (e.g. GFP, YFP, RFPs) tagging select cells and processes. Our tools preserve fluorescent proteins exceptionally well, and work with a variety of tissue and sample types, including: spinal cord, kidney, liver, heart, lung, intestine, organoids, and tumors.

Right: Thy1-driven YFP expression in an adult mouse brain. Sample courtesy of Dr. G. Allan Johnson, Duke Center for In Vivo Microscopy.

We have perfected technology to label and image c-FOS+ cells throughout the CNS. Using our robust models for detecting c-FOS+ cells in mouse brains, we provide: cell counts, densities, and heat maps quantifying levels of regional neural activity registered to the Allen Brain Atlas.

Left: c-FOS expression in rat hippocampus.

LifeCanvas can process and analyze samples for β-amyloid deposition in Alzheimer’s disease models, and for accumulation of α-synuclein in Lewy Bodies in Parkinson’s disease models. We determine levels of protein aggregation for regionally specific brain areas registered to the Allen Brain Atlas.

Left: GFAP (cyan) and Beta-amyloid plaques (magenta) in 5xFAD mouse brain model of Alzheimer’s disease. 15X, scale bar = 100 µm. Sample courtesy of Drs. David Elliott and Jonathan Epp, University of Calgary.

Our versatile technologies allow us to label multiple targets, including specific cell types (e.g. neurons, glia), nuclei, and vasculature. We can also process multiple organ or sample types using tissue-specific markers.

Left: Mouse duodenum segment, labeled with lectin (red, vasculature) and anti-Olfm4 (cyan, stem cell marker). Sample courtesy of Dr. Suhail Chaudhry, Ferrara Bone Marrow Transplantation Lab, Icahn School of Medicine.

Header: c-FOS (green) whole mouse brain mapping upon chemogenetic stimulation of tdTomato-labelled BSX neurons (red). Sample courtesy of Dr. Mathias Treier, Max-Delbrück Center for Molecular Medicine, Berlin.