CRO

Subpopulation of parvalbumin (PV) -expressing GABAergic neurons visible throughout mouse hemisphere labelled with anti-PV, 3.6X.

Whole Organ Mapping

LifeCanvas provides exceptional-quality whole organ mapping services to meet and surpass your research goals. We possess extensive expertise in tissue processing and imaging, and robust image analysis methods for complex, information-rich datasets. Just send us your PFA-fixed samples, and we’ll do the rest.

Left: Mouse spinal cord labeled with tdTomato. Sample courtesy of Lai Lab, UT Southwestern. Scale bar = 300 µm.

Leverage our comprehensive pipeline

Figure 1. SHIELD cross-links biological samples.
Tissue Preservation
Delipidation
Delipidation
Immunolabeling
Immunolabeling
Clearing
Optical Clearing
Imaging
Light Sheet Imaging
analysis
3D Image Analysis

Featured Applications

We have perfected technology to label and image c-FOS+ cells throughout the CNS. We also offer robust models for detecting c-FOS+ cells in murine brain samples and provide cell counts, densities, and heat maps quantifying levels of regional neural activity registered to the Allen Brain Atlas.

Right: Hippocampus and cortex in sagittal plane of mouse brain left hemisphere stained with anti-c-FOS (white), with detected cells in red. 3.6X, scale bar = 500 µm.

LifeCanvas offers researchers the opportunity to process and analyze samples for β-amyloid deposition (e.g. in Alzheimer’s disease) and for the accumulation of α-synuclein in Lewy Bodies (e.g. in Parkinson’s disease). Images are registered to the Allen Brain Atlas and levels of protein aggregation are determined for regionally specific brain areas.

Left: GFAP (cyan) and Beta-amyloid plaques (magenta) in 5xFAD mouse brain model of Alzheimer’s disease. 15X, scale bar = 100 µm. Sample courtesy of Epp Lab, University of Calgary.

LifeCanvas can clear and image brain samples which include endogenous fluorescent reporters tagging select cells and processes. Our versatile technologies can be employed to label multiple targets, including specific cell types (e.g. neurons, glia), nuclei, and vasculature. We can also process multiple organ or sample types using tissue-specific markers.

Right: Mouse duodenum segment, labeled with lectin (red, vasculature) and anti-Olfm4 (cyan, stem cell marker). Sample courtesy of Dr. Chaudhry, Ferrara Bone Marrow Transplantation Lab, Icahn School of Medicine.