Menu
LifeCanvas provides exceptional-quality whole organ mapping services to meet and surpass your research goals. Our team has extensive expertise in tissue processing and imaging, as well as robust image analysis methods for complex, information-rich datasets. Just send us your PFA-fixed samples, and we’ll do the rest.
Pictured above: Mouse brain hemisphere with ChAT+ (magenta) neurons in brainstem and basal forebrain, with dense labeling of NPY (cyan) throughout hypothalamus. Tissue courtesy of Dr. Hongwei Dong, Center for Integrative Connectomics (CIC), USC.
Our interactive data delivery platform presents your data in an easily digestible and customizable format. View the experimental effect on multiple brain regions in side-by-side bar plots, and compare treatment groups with subtracted heatmaps.
We deliver:
We can clear and image intact samples with endogenous fluorescent reporters (e.g. GFP, YFP, RFPs) tagging select cells and processes. Our tools preserve fluorescent proteins exceptionally well, and work with a variety of tissue and sample types, including: spinal cord, kidney, liver, heart, lung, intestine, organoids, and tumors.
Right: Thy1-driven YFP expression in an adult mouse brain. Sample courtesy of Dr. G. Allan Johnson, Duke Center for In Vivo Microscopy.
We have perfected technology to label and image c-FOS+ cells throughout the CNS. Using our robust models for detecting c-FOS+ cells in mouse brains, we provide: cell counts, densities, and heat maps quantifying levels of regional neural activity registered to the Allen Brain Atlas.
Right: c-FOS (green) expression upon chemogenetic stimulation of tdTomato-labelled BSX neurons (red). Sample courtesy of Dr. Mathias Treier, Max-Delbrück Center for Molecular Medicine, Berlin.
LifeCanvas can process and analyze samples for β-amyloid deposition in Alzheimer’s disease models, and for accumulation of α-synuclein in Lewy Bodies in Parkinson’s disease models. We determine levels of protein aggregation for regionally specific brain areas registered to the Allen Brain Atlas.
Right: β-amyloid disease plaques in magenta, astrocytes (GFAP) in cyan, in brain of 7-month-old homozygous ARTE10 male mouse from Taconic Biosciences, Alzheimer’s disease model #16347.
Our versatile technologies allow us to label multiple targets, including specific cell types (e.g. neurons, glia), nuclei, and vasculature. We can also process multiple organ or sample types using tissue-specific markers.
Right: Mouse duodenum segment, labeled with lectin (red, vasculature) and anti-Olfm4 (cyan, stem cell marker). Sample courtesy of Dr. Suhail Chaudhry, Icahn School of Medicine.