Header: Subpopulation of parvalbumin (PV) -expressing GABAergic neurons visible throughout mouse hemisphere labelled with anti-PV, 3.6X. Left: Mouse spinal cord labeled with tdTomato. Sample courtesy of Dr. Helen Lai, UT Southwestern. Scale bar = 300 µm.
We can clear and image intact samples with endogenous fluorescent reporters (e.g. GFP, YFP, RFPs) tagging select cells and processes. Our tools preserve fluorescent proteins exceptionally well, and work with a variety of tissue and sample types, including: spinal cord, kidney, liver, heart, lung, intestine, organoids, and tumors.
Right: Thy1-driven YFP expression in an adult mouse brain. Sample courtesy of Dr. G. Allan Johnson, Duke Center for In Vivo Microscopy.
We have perfected technology to label and image c-FOS+ cells throughout the CNS. Using our robust models for detecting c-FOS+ cells in mouse brains, we provide: cell counts, densities, and heat maps quantifying levels of regional neural activity registered to the Allen Brain Atlas.
Left: c-FOS expression in rat hippocampus.
LifeCanvas can process and analyze samples for β-amyloid deposition in Alzheimer’s disease models, and for accumulation of α-synuclein in Lewy Bodies in Parkinson’s disease models. We determine levels of protein aggregation for regionally specific brain areas registered to the Allen Brain Atlas.
Left: GFAP (cyan) and Beta-amyloid plaques (magenta) in 5xFAD mouse brain model of Alzheimer’s disease. 15X, scale bar = 100 µm. Sample courtesy of Drs. David Elliott and Jonathan Epp, University of Calgary.
Our versatile technologies allow us to label multiple targets, including specific cell types (e.g. neurons, glia), nuclei, and vasculature. We can also process multiple organ or sample types using tissue-specific markers.
Left: Mouse duodenum segment, labeled with lectin (red, vasculature) and anti-Olfm4 (cyan, stem cell marker). Sample courtesy of Dr. Suhail Chaudhry, Ferrara Bone Marrow Transplantation Lab, Icahn School of Medicine.