Tissue Clearing & Labeling

Tissue Clearing Reagents

Our new Clear+ tissue clearing technique is the only method that delipidates samples with no change in morphology and with minimal impact on structural integrity. Fully delipidate whole mouse brains or comparably sized samples in just one day with SmartBatch+, or in one week with our passive clearing kit.

SmartBatch+

Our turn-key SmartBatch+ system combines electrophoretic tissue clearing and immunolabeling into one high-throughput device. Leverage the Clear+ tissue clearing method, along with eFLASH and patented stochastic electrotransport technologies, to rapidly clear and label whole organs. Key highlights and features include:

Batch processing of up to 12 whole mouse brains, 4 whole rat brains, or comparably sized samples
Uniform labeling with no surface saturation of antibodies or dyes, and minimal antibody usage
No immunolabeling necessary for endogenous fluorescence (no fluorescent protein quenching)
1-day active tissue clearing, 2-day active immunolabeling (primary & secondary)

EasyIndex

Our preformulated EasyIndex solution raises and homogenize the refractive index of delipidated tissue samples, rendering them fully transparent. This enables light penetration into the sample and ensures the acquisition of high-resolution, in-focus image data.

Recent publications citing LifeCanvas tissue clearing & labeling techniques

A Light sheet fluorescence microscopy and machine learning-based approach to investigate drug and biomarker distribution in whole organs and tumors

Niyanta Kumar, Petr Hrobar, Martin Vagenknecht, Jindrich Soukup, Nadia Patterson, Peter Bloomingdale, Tomoko Freshwater, Sophia Bardehle, Roman Peter, Ruban Mangadu, Cinthia V Pastuskovas, Chiswili Yves Chabu, Mark T. Cancilla

Journal:

bioRxiv

Publication Date:

September 17, 2023

Research Area:

Neurodegeneration, Cancer, Vasculature, Methods

Tissue Species:

Mouse

Tissue Type:

Brain, Tumor

Products Cited:

SHIELD, EasyIndex, SmartClear, SmartLabel, SmartSPIM, SmartAnalytics, CRO Services

Developmental Mouse Brain Common Coordinate Framework

Fae A. Kronman, Josephine K. Liwang, Rebecca Betty, Daniel J. Vanselow, Yuan-ting Wu, Nicholas J. Tustison, Ashwin Bhandiwad, Steffy B. Manjila, Jennifer A. Minteer, Donghui Shin, Choong Heon Lee, Rohan Patil, Jeffrey T. Duda, Luis Puelles, James C. Gee, Jiangyang Zhang, Lydia Ng, Yongsoo Kim

Journal:

bioRxiv

Publication Date:

September 15, 2023

Research Area:

Methods, Other

Tissue Species:

Mouse

Tissue Type:

Brain

Products Cited:

SHIELD, EasyIndex, SmartClear, SmartLabel, Passive Clearing Kit, SmartSPIM

Drugs of abuse hijack a mesolimbic pathway that processes homeostatic need

Bowen Tan, Caleb J. Browne, Tobias Nöbauer, Alipasha Vaziri, Jeffrey M. Friedman, Eric J. Nestler

Journal:

bioRxiv

Publication Date:

September 5, 2023

Research Area:

Neural Circuits

Tissue Species:

Mouse

Tissue Type:

Brain

Products Cited:

SHIELD, EasyIndex, SmartBatch+, SmartSPIM, CRO Services