Tissue Clearing & Labeling

Delipidation and conduction buffers for tissue clearing

Our new Clear+ tissue clearing technique is the only method that delipidates samples with no change in morphology and with minimal impact on structural integrity. Fully delipidate whole mouse brains or comparably sized samples in just one day with SmartBatch+, or in one week with our passive clearing kit.

A black machine with a digital control panel displaying blue screens and buttons. "LifeCanvas Technologies" logo on the front.

Our turn-key SmartBatch+ system combines electrophoretic tissue clearing and immunolabeling into one high-throughput device. Leverage the Clear+ tissue clearing method, along with eFLASH and patented stochastic electrotransport technologies, to rapidly clear and label whole organs. Key highlights and features include:

  • Batch processing of up to 12 whole mouse brains, 4 whole rat brains, or comparably sized samples
  • Uniform labeling with no surface saturation of antibodies or dyes, and minimal antibody usage
  • No immunolabeling necessary for endogenous fluorescence (no fluorescent protein quenching)
  • 1-day active tissue clearing, 2-day active immunolabeling (primary & secondary)

Our SeTau-647 Secondary Antibody is the only commercially available pre-conjugated SeTau Secondary that will provide superior photostability when labeling. Ideal for light sheet, 2P Imaging, expansion tissues, super-resolution, or live cell imaging where fluorescence stability is paramount.

Specifications:

  • Antibody Format: Whole IgG
  • Clonality: Polyclonal
  • Host: Donkey
  • Minimal Cross Reactivity: Bovine, chicken, goat, guinea pig, syrian hamster, horse, human, mouse, rat, sheep serum proteins (except for reactive target)
  • Physical State: Lyophilized solid
  • Provided in 0.5 mg vials.
EasyIndex for Index Matching

Our preformulated EasyIndex solution raises and homogenize the refractive index of delipidated tissue samples, rendering them fully transparent. This enables light penetration into the sample and ensures the acquisition of high-resolution, in-focus image data.

Recent publications citing LifeCanvas tissue clearing & labeling techniques

An anatomical map of squid lateral lines

Haoming Wang, Carsten Wolff, Nicolas Pintozzi, Anna-Maria Petriv, Brian M. McDermott Jr.

Journal:
Current Biology
Publication Date:
Products Cited:
SHIELD, EasyIndex, SmartBatch+, MegaSPIM, Radiant, SeTau Secondary
Topographic structure and function of locus coeruleus noradrenaline neurons

Zhixiao Su, Polina Kosillo, Kanghoon Jung, Shuonan Chen, Mathew T. Summers, Alex Piet, Han Hou, Kenta M. Hagihara, Drew Friedmann, Olivia Ho-Shing, Matthew I. Becker, Thomas Chartrand, Xinxin Yin, Peter Grotz, Ella Hilton-VanOsdall, Margaret Lee, Rajvi Javeri, Samantha L. Tuggle, Naveen Ouellette, Holly Myers, Judith Baka, Camilo Laiton, Kaelin Wulf, John Rohde, Alessio P. Buccino, Cameron Arshadi, Di Wang, Sharmishtaa Seshamani, Sonya Vasquez, Carolyn M. Eng, Douglas R. Ollerenshaw, Nick Dee, Tamara Casper, Windy Ho, Matthew Jungert, Atlas Jordan, Elliot Phillips, Anish Bhaswanth Chakka, Kamiliam Nasirova, Krista Blake, Audrey McCutcheon, Megan Koch, Maria Camila Vergara, Kimberly A. Smith, Tim Jarsky, Nicholas Lusk, Mara C. P. Rue, Xiaoyin Chen, Joshua H. Siegle, Adam K. Glaser, Brian R. Lee, Karel Svoboda, Yoh Isogai, Jayaram V. Chandrashekar & Jeremiah Y. Cohen

Journal:
Nature
Publication Date:
Research Area:
Neural Circuits
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
EasyIndex, SmartBatch+, SmartSPIM
A queen odour mediates reproductive suppression in a eusocial mammal

Mohammed A. Khallaf, Daniel W. Hart, Wenhan Luo, Firdevs Murad, Felipe Cybis Pereira, Daniel Mendez-Aranda, Nicole Hagenah, Alice Rossi, Valérie Bégay, Jan Okrouhlík, Dietmar Krautwurst, Mungo Kisinza Ngalameno, Andre Ganswindt, Alison J. Barker, Radim Šumbera, Markus Knaden, Sophie Pezet, Andrew Woehler, Bill S. Hansson, Nigel C. Bennett & Gary R. Lewin

Journal:
Nature
Publication Date:
Research Area:
Neural Activity
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartClear, SmartLabel, SmartBatch+, Passive Clearing Kit, SmartSPIM, CRO Services