Tissue Clearing & Labeling

Tissue Clearing Buffers

Our new Clear+ tissue clearing technique is the only method that delipidates samples with no change in morphology and with minimal impact on structural integrity. Fully delipidate whole mouse brains or comparably sized samples in just one day with SmartBatch+, or in one week with our passive clearing kit.

SmartBatch+

Our turn-key SmartBatch+ system combines electrophoretic tissue clearing and immunolabeling into one high-throughput device. Leverage the Clear+ tissue clearing method, along with eFLASH and patented stochastic electrotransport technologies, to rapidly clear and label whole organs. Key highlights and features include:

  • Batch processing of up to 12 whole mouse brains, 4 whole rat brains, or comparably sized samples
  • Uniform labeling with no surface saturation of antibodies or dyes, and minimal antibody usage
  • No immunolabeling necessary for endogenous fluorescence (no fluorescent protein quenching)
  • 1-day active tissue clearing, 2-day active immunolabeling (primary & secondary)

Our SeTau-647 Secondary Antibody is the only commercially available pre-conjugated SeTau Secondary that will provide superior photostability when labeling. Ideal for light sheet, 2P Imaging, expansion tissues, super-resolution, or live cell imaging where fluorescence stability is paramount.

Specifications:

  • Antibody Format: Whole IgG
  • Clonality: Polyclonal
  • Host: Donkey
  • Minimal Cross Reactivity: Bovine, chicken, goat, guinea pig, syrian hamster, horse, human, mouse, rat, sheep serum proteins (except for reactive target)
  • Physical State: Lyophilized solid
  • Provided in 0.5 mg vials.
EasyIndex bottle

Our preformulated EasyIndex solution raises and homogenize the refractive index of delipidated tissue samples, rendering them fully transparent. This enables light penetration into the sample and ensures the acquisition of high-resolution, in-focus image data.

Recent publications citing LifeCanvas tissue clearing & labeling techniques

Adeno-associated virus delivered CXCL9 sensitizes glioblastoma to anti-PD-1 immune checkpoint blockade

Christina A. von Roemeling, Jeet A. Patel, Savannah L. Carpenter, Oleg Yegorov, Changlin Yang, Alisha Bhatia, Bently P. Doonan, Rylynn Russell, Vrunda S. Trivedi, Kelena Klippel, Daniel H. Ryu, Adam Grippin, Hunter S. Futch, Yong Ran, Lan B. Hoang-Minh, Frances L. Weidert, Todd E. Golde & Duane A. Mitchell

Journal:
Nature Communications
Publication Date:
Products Cited:
SmartBatch+, Passive Clearing Kit, SmartSPIM, CRO Services
Serine racemase deletion alters adolescent social behavior and whole-brain cFos activation

Stephanie E. Brown, Ziyi (Zephyr) Wang, Emily L. Newman, Elif Engin, Sabina Berretta, Darrick T. Balu, Oluwarotimi O. Folorunso.

Journal:
Frontiers in Psychiatry
Publication Date:
Research Area:
cFos, Mapping
Tissue Species:
Mouse
Tissue Type:
Heart
Products Cited:
SHIELD, SmartBatch+, SmartSPIM, CRO Services
A cocaine-activated ensemble exerts increased control over behavior while decreasing in size

Kimberly C. Thibeault, Michael Z. Leonard, Veronika Kondev, Soren D. Emerson, Rishik Bethi, Alberto J. Lopez, Jonathon P. Sens, Brett P. Nabit, Hannah B. Elam, Danny G. Winder, Sachin Patel, Drew D. Kiraly, Brad A. Grueter, Erin S. Calipari.

Journal:
Biological Psychiatry
Publication Date:
Research Area:
cFos, Mapping
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, SmartBatch+, SmartSPIM, CRO Services