Tissue Clearing & Labeling

Tissue Clearing + Tissue Labeling - Highlighted pipeline icons
Tissue Clearing Buffers

Our new Clear+ tissue clearing technique is the only method that delipidates samples with no change in morphology and with minimal impact on structural integrity. Fully delipidate whole mouse brains or comparably sized samples in just one day with SmartBatch+, or in one week with our passive clearing kit.

SmartBatch+ with lid closed

Our turn-key SmartBatch+ system combines electrophoretic tissue clearing and immunolabeling into one high-throughput device. Leverage the Clear+ tissue clearing method, along with eFLASH and patented stochastic electrotransport technologies, to rapidly clear and label whole organs. Key highlights and features include:

  • Batch processing of up to 12 whole mouse brains, 4 whole rat brains, or comparably sized samples
  • Uniform labeling with no surface saturation of antibodies or dyes, and minimal antibody usage
  • No immunolabeling necessary for endogenous fluorescence (no fluorescent protein quenching)
  • 1-day active tissue clearing, 2-day active immunolabeling (primary & secondary)
SmartClear II Pro

This legacy active tissue clearing system employs patented stochastic electrotransport technology to rapidly and uniformly remove light-scattering membrane lipids. SmartClear uses the Clear+ tissue clearing method and prevents sample deformation and precipitate formation.

SmartLabel

This legacy active immunolabeling system achieves uniform whole-organ antibody labeling by combining our patented stochastic electrotransport method with SWITCH. Compared to passive labeling techniques such as iDISCO and CUBIC, SmartLabel provides superior penetration and uniformity of probes in just 1-2 days.

Recent publications citing LifeCanvas tissue clearing & labeling techniques

Loss of insulin signaling in astrocytes exacerbates Alzheimer-like phenotypes in a 5xFAD mouse model

Wenqiang Chen, Qian Huang, Ekaterina Katie Lazdon, Antonio Gomes, Marisa Wong, Emily Stephens, Tabitha Grace Royal, Dan Frenkel, Weikang Cai, C. Ronald Kahn

Journal:
PNAS
Publication Date:
Research Area:
Neurodegeneration, Metabolism
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, Passive Clearing Kit, SmartSPIM, SmartAnalytics, CRO Services
Leveraging the Power of 3D Brain-Wide Imaging and Mapping Tools for Brain Injury Research in Murine Models

Mehwish Anwer, Jeffrey LeDue, Zefang Wang, Sarah Wang, Wai Hang Cheng, Mariia Burdyniuk, Honor Cheung, Jianjia Fan, Carlos Barron, Peter A. Cripton, Mark S. Cembrowski, Fabio Rossi, Timothy H. Murphy, Cheryl L. Wellington

Journal:
bioRxiv
Publication Date:
Research Area:
Brain Injury, Methods
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, SmartAnalytics, CRO Services
BNST PKCδ neurons are activated by specific aversive conditions to promote anxiety-like behavior

Kellie M. Williford, Anne Taylor, James R. Melchior, Hye Jean Yoon, Eryn Sale, Milen D. Negasi, Danielle N. Adank, Jordan A. Brown, Michelle N. Bedenbaugh, Joseph R. Luchsinger, Samuel W. Centanni, Sachin Patel, Erin S. Calipari, Richard B. Simerly, Danny G. Winder

Journal:
Neuropsychopharmacology
Publication Date:
Research Area:
Neural Circuits
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, Passive Clearing Kit, SmartSPIM, SmartAnalytics