Video Gallery

Sample was cleared with Clear+ technique and imaged at 3.6X with SmartSPIM. Sample courtesy of Shenfeng Qiu at the University of Arizona College of Medicine.

β-amyloid disease plaques in magenta, astrocytes (GFAP) in cyan, in brain of 7-month-old homozygous ARTE10 male mouse from Taconic Biosciences, Alzheimer’s disease model #16347. Cleared and labeled with our pipeline, imaged with SmartSPIM at 15X.

β-amyloid disease plaques in magenta, astrocytes (GFAP) in cyan, in brain of 7-month-old homozygous ARTE10 male mouse from Taconic Biosciences, Alzheimer’s disease model #16347. Cleared and labeled with our pipeline, imaged with SmartSPIM at 4X.

Uniform whole brain staining was achieved with only 3.5 µg of anti-cFOS primary antibody. Imaged at 1.8 µm XY pixel size and 4 µm z-step.

TH staining of an intact mouse brain hemisphere, labeling the Ventral Tegmental Area, Substantia Nigra, Retrorubral Field, and Locus Coeruleus, regions highly populated with dopaminergic and noradrenergic neurons. Dataset acquired at 1.8 x 1.8 x 4 µm voxel size.

Cortical neurons, stained with NeuN (magenta), are interspersed with microglia, stained with IBA1 (cyan). Imaged at 15X magnification, 0.41 µm pixel size, and 1 µm z-step.

15X objective captures mouse brain astrocytes. Voxel size is 0.41 µm x 0.41 µm x 1 µm (z-step).

Tissue stained with a lectin dye labeling blood vessels with high specificity. A 1.5 x 1.5 x 1.8 mm region was acquired with a 1 µm Z-step in <5 minutes. 15X magnification.

Imaged at 4 µm Z-step and 1.8 µm XY pixel size.

 

Tissue courtesy of Gord Fishell, Broad Institute.

Mouse brain hemisphere displaying ChAT-positive neurons (magenta) in both the brainstem and basal forebrain and dense labeling of Neuropeptide Y (cyan) throughout the hypothalamus. Imaged at 4 µm Z-step and 1.8 µm XY pixel size.

 

Tissue courtesy of Hongwei Dong, Center for Integrative Connectomics (CIC), USC.

Mouse duodenum segment perfused with lectin (red) to label vasculature and immunolabeled with anti-Olfm4 (stem cell marker, cyan). Imaged at 2 µm Z-step and 1.8 µm XY pixel size.

 

Sample courtesy of Suhail Chaudhry of the Ferrara Bone Marrow Transplantation Lab, Icahn School of Medicine.

Stained with nuclear stain (SYTO16, cyan) and vasculature stain (DyLight 649-conjugated tomato lectin, pink) in <1 day.

 

Tissue courtesy of the Translational Bioimaging Group, Barrow Neurological Institute.

Immunolabeling of an intact mouse brain hemisphere using anti-GFP primary antibody & Fab fragment secondary antibody. Imaged at 1.8 μm/pixel in XY with 4-μm Z-step.

Immunolabeling of an intact mouse brain hemisphere using anti-Parvalbumin (PV) primary antibody & a Fab fragment secondary antibody. Imaged at 1.8 µm/pixel in XY with 4 µm Z-step.

2-mm thick slab of mouse brain immunostained for GFAP. Imaged at 1.8 µm/pixel in XY with 2 µm Z-steps.

Mouse brain labeled with vascularly delivered fluorescent dye, imaged at 10X.

 

Sample courtesy of David Kleinfeld and Byungkook Lim, UCSD.

tdTomato expression driven by the Calb2 (calretinin) promoter reveals circuits formed by subtypes of inhibitory interneurons in a mouse brain hemisphere. Imaged at 1.8 µm/pixel in XY with 4 µm Z-step.

 

Sample courtesy of Wei Xu, UT Southwestern.

Staining of a mouse cerebral hemisphere using green fluorescent nuclear dye (SYTO 16). Staining of astrocytes traces out the brain’s network of vasculature. Imaged with 1.8 µm/pixel in XY and 4 µm Z-step.

Tissue courtesy of Rogers Lab at Harvard School of Public Health.

Entire hemisphere was stained using SYTO 16 green fluorescent nucleic acid stain. Cleared and stained with our pipeline, imaged with SmartSPIM in less than 1 hour. High and uniform axial resolution allows for clear delineation of cell bodies in XY and importantly in Z.