SmartClear II Pro

Active tissue clearing made easy

SmartClear II Pro is ready-to-use, an order of magnitude faster than passive clearing, and provides optimal imaging and staining quality. Our SmartClear system effectively delipidates for best antibody diffusion and minimal light scatter, and ensures maximum preservation of fluorescent protein signals with optimized buffers that maintain pH.

Patent-pending nanoporous membranes prevent common clearing issues including tissue damage, contamination, browning, black precipitates, and deformation.

Efficient and cost-effective

SmartClear II Pro can clear 4 whole mouse brains, 2 rat brains, or similarly sized samples simultaneously. Its customizable larger clearing chamber enables clearing of various sample types and sizes to suit your needs.

Our Clear+ tissue clearing buffer set maximizes optical transparency, minimizes clearing time, and lasts ~10 days without loss of clearing ability. Plus, there is no tissue shrinkage or expansion!

Highly compatible

SmartClear II Pro works with many tissue preservation techniques which avoid the loss of endogenous fluorescence and tissue shrinkage present in organic solvent-based clearing methods, including:

Uniform, rapid intact tissue staining can then be achieved SmartBatch+ or SmartLabel.

Set up your device

Protocols

Documentation

Recent publications citing SmartClear

Nudell et al. “HYBRiD: hydrogel-reinforced DISCO for clearing mammalian bodies.” Nature Methods (March 2022).

Nobre et al. “MiRNA-Mediated Knockdown of ATXN3 Alleviates Molecular Disease Hallmarks in a Mouse Model for Spinocerebellar Ataxia Type 3.” Nucleic Acid Therapeutics (December 2021).
Menzel et al. “Lymphocyte access to lymphoma is impaired by high endothelial venule regression.” Cell Reports (October 2021).
Foster et al. “The Mouse Cortico-Basal Ganglia-Thalamic Network.” Nature (October 2021).
Muñoz-Castañeda et al. “Cellular Anatomy of the Mouse Primary Motor Cortex.” Nature (October 2021).