3D Histology & Imaging Free Trial

High-quality spatial data begins with effective tissue processing and imaging tools. Many tissue clearing methods are not only laborious and error-prone, but also damage your samples and key molecular characteristics like proteins, probes, and antigenicity. That’s why we developed our Clear+ system: to preserve these features all the way through the tissue preparation process, while saving precious time and resources.

Our proprietary tissue preservation and clearing methods, combined with advanced light sheet microscopy techniques, generate unbiased 3D data for a variety of research applications – from neuroscience to cancer, regenerative medicine, and beyond.

tdTomato traces ennervation in whole mouse head with attached skull and spine, processed with modified SHIELD & Clear+ method by Dr. Jun Takato at MIT

eGFP-expressing motor cortical neurons in whole mouse brain, imaged with SmartSPIM light sheet microscope, 10x objective — Retrogradely-labeled motor cortical neurons projecting to striatum using rabies virus expressing eGFP, 10x objective. Sample courtesy of Dr. Byungkook Lim, UCSD.

NPY and ChAT in intact mouse right brain hemisphere — Mouse brain hemisphere displaying ChAT-positive neurons in both the brainstem and basal forebrain and dense labeling of Neuropeptide Y throughout the hypothalamus. Tissue immunolabeled anti-NPY (cyan) and anti-ChAT (magenta). Tissue courtesy of Hongwei Dong, Bin Zhang, and Neda Khanjani and the Center for Integrative Connectomics (CIC) lab.

Astrocytes and β-amyloid plaques in a 5xFAD intact mouse brain model of Alzheimer’s disease, imaged with SmartSPIM light sheet microscope, 3.6X objective — Immunolabeled GFAP (cyan) and Beta-amyloid plaques (magenta) in a 5xFAD mouse brain model of Alzheimer’s disease, 4X objective. Sample courtesy of Epp Lab, University of Calgary.

Intact mouse duodenum perfused with lectin and immunolabeled with anti-Olfm4 — Mouse duodenum segment, perfused with lectin (red) to label vasculature and immunolabeled with anti-Olfm4 stem cell marker (cyan). Imaged at 2 µm Z-step and 1.8 µm XY pixel size.

tdTomato labeling of mouse spinal cord cell-type in intact sample — tdTomato labeling of mouse spinal cord cell-type. Sample courtesy of Dr. Helen Lai, UT Southwestern.

Thy1-driven YFP expression in whole mouse brain — Thy1-driven YFP expression in adult mouse brain. Sample courtesy of Dr. G. Allan Johnson, Duke Center for In Vivo Microscopy.

Astrocytes and β-amyloid plaques in intact mouse brain model of Alzheimer's disease, imaged with SmartSPIM light sheet microscope, 15X objective — GFAP (magenta) and β-amyloid (cyan) in brain of 7-month-old homozygous ARTE10 male mouse (model #16347) from Taconic Biosciences.

Interested in testing out our tools in your own lab? Please fill out the form below with as much detail as possible, and one of our experts will be in touch shortly. Our comprehensive free trial includes:

*Available for intact organ samples. Imaging for other sample types may be available on request.