Immunohistochemistry (IHC) and 3D histology are different approaches to detecting specific proteins in biological tissue.
Published by Park et al. in Nature Biotechnology 2018, our SHIELD reagent (AKA GE38) safeguards endogenous fluorescence, protein antigenicity, nucleic acids, and overall tissue architecture against physical and chemical stressors. The SHIELD tissue preservation method also protects tissue during fluorescent protein imaging and antibody labeling across multiple rounds.
Compatible with:
Our streamlined protocol takes just 4-6 days, without the variability of tissue embedding via hydrogel or paraffin. Reagents are non-toxic and can be easily disposed of after use.
250 mL: Preserves 25 whole mouse brains or comparably sized samples.
500 mL: Preserves 50 whole mouse brains or comparably sized samples.
Dong Yang, Yu Wang, Tianbo Qi, Xi Zhang, Leyao Shen, Jingrui Ma, Zhengyuan Pang, Neeraj K. Lal, Daniel B. McClatchy, Saba Heydari Seradj, Verina H. Leung, Kristina Wang, Yi Xie, Filip S. Polli, Anton Maximov, Oscar Christian Gonzalez, Luis de Lecea, Hollis T. Cline, Vineet Augustine, John R. Yates III, and Li Ye.
Rhiana C. Simon, Weston T. Fleming, Pranav Senthilkumar, Brandy A. Briones1,2, Kentaro K. Ishii, Madelyn M. Hjort, Madison M. Martin, Koichi Hashikawa, Andrea D. Sanders, Sam A. Golden, Garret D. Stuber.
Maxime W C Rousseaux, Haley Geertsma, Zoe Fisk, Lillian Sauline, Alice Prigent, Kevin Kurgat, Steve Callaghan, Julianna Tomlinson, Michael Henderson
Immunohistochemistry (IHC) and 3D histology are different approaches to detecting specific proteins in biological tissue.
The SHIELD kit provides optimal tissue preservation by stabilizing molecular features & tissue architecture with flexible epoxide bonds.
Without SHIELD preservation, many tissue clearing techniques can lead to significant sample degradation and loss of biomolecular information.
Immunohistochemistry methods are laborious, error-prone, and expensive – intact tissue processing technologies resolve many of these issues.
For technical support reach out to our dedicated customer support team at smartspim@lifecanvastech.com