Here, we demonstrate how the Megatome Premium can easily facilitate this process through whole-mount and non-destructive tissue sectioning.
Published by Park et al. in Nature Biotechnology 2018, our SHIELD reagent (AKA GE38) safeguards endogenous fluorescence, protein antigenicity, nucleic acids, and overall tissue architecture against physical and chemical stressors. The SHIELD tissue preservation method also protects tissue during fluorescent protein imaging and antibody labeling across multiple rounds.
Compatible with:
Our streamlined protocol takes just 4-6 days, without the variability of tissue embedding via hydrogel or paraffin. Reagents are non-toxic and can be easily disposed of after use.
250 mL: Preserves 25 whole mouse brains or comparably sized samples.
500 mL: Preserves 50 whole mouse brains or comparably sized samples.
Abhishek Dubey, Erika Yamashita, Biljana Stangeland, Imane Abbas, David Fooksman, Robert A. Harris, Gregory M. Palmer, Wade R. Koba, Jinghang Zhang, Benjamin T. Himes, Olivia R. Lu, Winson S. Ho, Raoul V. Kuiper, Derek Huffman, Zhiping Wu, Yutaka Uchida, Masaru Ishii, Rachel L. Welch, Alexander F. Fiedler, David Reynolds, S. A. Mohieb Hosainey, Kostantin Dobrenis, Qinge Ye, Kevin Fisher, Nathaniel Killian, E. Richard Stanley, Emad Eskandar & Jinan Behnan
Aishwarya Patwardhan, Siyao Li, Jessica Chen, Katrina Y. Choe
Hanan Mohammed, Sadaf Usmani, Brij Bhushan, Anique Ahmad, Oraib Al-Ketan, Ahmed A. Shibl, Maylis Boitet, Devjoy Dev, Heba Naser, Aashish R. Jha, Khalil B. Ramadi
Here, we demonstrate how the Megatome Premium can easily facilitate this process through whole-mount and non-destructive tissue sectioning.
Immunohistochemistry (IHC) and 3D histology are different approaches to detecting specific proteins in biological tissue.
The SHIELD kit provides optimal tissue preservation by stabilizing molecular features & tissue architecture with flexible epoxide bonds.
Without SHIELD preservation, many tissue clearing techniques can lead to significant sample degradation and loss of biomolecular information.
For technical support reach out to our dedicated customer support team at support@lifecanvastech.com.