SHIELD

Tissue Preservation - Highlighted pipeline icon

Exceptional tissue preservation

Published by Park et al. in Nature Biotechnology 2018, our SHIELD reagent (AKA GE38) safeguards endogenous fluorescence, protein antigenicity, nucleic acids, and overall tissue architecture against physical and chemical stressors. The SHIELD tissue preservation method also protects tissue during fluorescent protein imaging and antibody labeling across multiple rounds.

Compatible with:

GFP+ motor cortical neurons in whole mouse brain (left) and tdTomato+ in a mouse spinal cord cell-type (right)
Left: GFP+ motor cortical neurons, sample courtesy of Dr. Byungkook Lim, UCSD. Right: RFP+ spinal cord cells, sample courtesy of Dr. Helen Lai, UT Southwestern. Both imaged with SmartSPIM.

Quick & easy tissue preservation protocol

Our streamlined protocol takes just 4-6 days, without the variability of tissue embedding via hydrogel or paraffin. Reagents are non-toxic and can be easily disposed of after use.

Figure showing SHIELD tissue preservation mechanism

Two SHIELD kit sizes

250 mL: Preserves 25 whole mouse brains or comparably sized samples.

  • 250 mL SH-Epoxy
  • 125 mL SH-Buffer
  • 500 mL SH-ON

500 mL: Preserves 50 whole mouse brains or comparably sized samples.

  • 500 mL SH-Epoxy
  • 250 mL SH-Buffer
  • 1000 mL SH-ON
SHIELD Kit for tissue preservation

Recent publications citing SHIELD

Brain tumors induce widespread disruption of calvarial bone and alteration of skull marrow immune landscape

Abhishek Dubey, Erika Yamashita, Biljana Stangeland, Imane Abbas, David Fooksman, Robert A. Harris, Gregory M. Palmer, Wade R. Koba, Jinghang Zhang, Benjamin T. Himes, Olivia R. Lu, Winson S. Ho, Raoul V. Kuiper, Derek Huffman, Zhiping Wu, Yutaka Uchida, Masaru Ishii, Rachel L. Welch, Alexander F. Fiedler, David Reynolds, S. A. Mohieb Hosainey, Kostantin Dobrenis, Qinge Ye, Kevin Fisher, Nathaniel Killian, E. Richard Stanley, Emad Eskandar & Jinan Behnan

Journal:
Nature Neuroscience
Publication Date:
Research Area:
Cancer
Tissue Species:
Mouse
Tissue Type:
Brain, Bone
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM
Three-dimensional quantification of oxytocin neurons in the hypothalamic paraventricular nucleus reveals sex- and subregion-specific differences in two genetic mouse models of autism

Aishwarya Patwardhan, Siyao Li, Jessica Chen, Katrina Y. Choe

Journal:
Journal of Neuroendocrinology
Publication Date:
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM
Passive intestinal microbiome sampling using an ingestible device with tortuous lattices

Hanan Mohammed, Sadaf Usmani, Brij Bhushan, Anique Ahmad, Oraib Al-Ketan, Ahmed A. Shibl, Maylis Boitet, Devjoy Dev, Heba Naser, Aashish R. Jha, Khalil B. Ramadi

Journal:
Cell Device
Publication Date:
Research Area:
Methods, Other
Tissue Species:
Mouse
Tissue Type:
GI Tract
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, CRO Services

Further reading