c-FOS Brain Mapping: Challenges & Solutions

Mapping the localization of increased c-FOS expression provides important insights into specific brain regions implicated in the responses to a range of stimuli. Researchers have used c-FOS protein up-regulation as an indicator for a variety of cellular activities. For instance, this marker is a useful proxy for measuring the mammalian response to various stimuli including… Continue reading c-FOS Brain Mapping: Challenges & Solutions

3D Phenotyping with the SmartSPIM Light Sheet Microscope

After tissue clearing and (optional) labeling, intact tissue needs to be index-matched with EasyIndex refractive index-matching solution. Afterwards, tissues will appear fully transparent and ready for imaging using the SmartSPIM light sheet microscope. To complete your workflow, LifeCanvas Technologies offers SmartAnalytics – a powerful machine learning analysis tool, specially designed for large 3D datasets. This… Continue reading 3D Phenotyping with the SmartSPIM Light Sheet Microscope

c-FOS: Molecular biology and detection for analysis of neuronal activity throughout the brain

An image stack captures the hippocampus and cortex in the sagittal plane of a mouse brain left hemisphere stained with anti-c-FOS. Acquired at 3.6X magnification and 4 µm z-step. Scale bar = 500 µm.

Although in the realm of neuroscience c-FOS protein is typically recognized as a marker of neuronal activity, it was originally discovered nearly forty years ago in rat fibroblasts (Curran and Teich, J. Virology, 1982) and characterized as being encoded by a proto-oncogene (a gene that once mutated can become tumor-inducing) and is overexpressed in a… Continue reading c-FOS: Molecular biology and detection for analysis of neuronal activity throughout the brain