c-FOS expression is an indicator of recent neural cell activity, making the detection of c-FOS+ cells a high priority for many neuroscientists. This image stack captures the hippocampus and cortex in the transverse plane of an intact mouse brain stained with anti-c-FOS. These images were acquired with SmartSPIM at 3.6X magnification and 4 µm z-step.
But ultimately, quantification is key. To complete the end-to-end tissue processing pipeline, LifeCanvas has developed SmartAnalytics, a powerful machine learning analysis tool designed for large 3D datasets. SmartAnalytics currently offers:
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Left: Heatmap of cell density by region in mouse brain hemisphere stained with anti-c-FOS, generated with SmartAnalytics. Right: Histogram of cell density by region in same c-FOS stained hemisphere, generated by SmartAnalytics.
Although in the realm of neuroscience c-FOS protein is typically recognized as a marker of neuronal activity, it was originally discovered nearly forty years ago in rat fibroblasts and characterized as being encoded by a proto-oncogene (a gene that once mutated can become tumor-inducing) and
In 1906 at the University of Munich the neuropathologist Dr. Alois Alzheimer [Figure 1] was studying the brain of a recently deceased patient, Auguste Deter. Previously Alzheimer had assisted Frau Deter in the city asylum in Frankfurt and observed that she suffered from memory loss
Acetylcholine neurotransmission in the brain via the central cholinergic system has been implicated in critical functions including cognition, arousal, attention and learning. Moreover, interest in central cholinergic transmission has been piqued given that loss of cholinergic innervation may play an important role in neurodegenerative disorders
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