c-FOS expression is an indicator of recent neural cell activity, making the detection of c-FOS+ cells a high priority for many neuroscientists. This image stack captures the hippocampus and cortex in the transverse plane of an intact mouse brain stained with anti-c-FOS. These images were acquired with SmartSPIM at 3.6X magnification and 4 µm z-step.
But ultimately, quantification is key. To complete the end-to-end tissue processing pipeline, LifeCanvas has developed SmartAnalytics, a powerful machine learning analysis tool designed for large 3D datasets. SmartAnalytics currently offers:
and there are more applications being developed all the time! Submit a Service Request form or schedule a consultation with one of our lead scientists to learn more about this technology.
Left: Heatmap of cell density by region in mouse brain hemisphere stained with anti-c-FOS, generated with SmartAnalytics. Right: Histogram of cell density by region in same c-FOS stained hemisphere, generated by SmartAnalytics.
Emerging organoid technologies have been used to model human organ development and diseases “in a dish”. Organoids are derived either from induced pluripotent or organ-restricted adult stem cells. These cells are self-renewing, self-organizing and able to grow into 3D structures resembling the architecture and physiology of the original biological specimens.
Over 190 million people have now been infected with the novel coronavirus SARS-CoV-2 in the ongoing coronavirus disease (COVID-19) pandemic with a total number of deaths passing 4.1 million worldwide. Multiple organs are affected by COVID-19, however, the lungs are the main site of disease.
The goal of histology has long been to interrogate complex structures in whole tissue samples. Over centuries, slicing of samples for traditional two-dimensional histology has been indispensable in understanding the structure and morphology of multiple tissue types. However, this approach is limited to yielding images in thin tissue sections, is time-consuming and is error-prone due to tissue distortion and information loss.
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