c-FOS expression is an indicator of recent neural cell activity, making the detection of c-FOS+ cells a high priority for many neuroscientists. This image stack captures the hippocampus and cortex in the sagittal plane of a mouse brain left hemisphere stained with anti-c-FOS. These images were acquired with SmartSPIM at 3.6X magnification and 4 µm z-step. Scale bar 500 µm.
But ultimately, quantification is key. To complete the end-to-end tissue processing pipeline, LifeCanvas has developed SmartAnalytics, a powerful machine learning analysis tool designed for large 3D datasets. The red dots in the video above indicate the results of a c-FOS cell detection algorithm applied to the SmartSPIM image dataset. SmartAnalytics currently offers:
and there are more applications being developed all the time! Submit a Service Request form or schedule a consultation with one of our lead scientists to learn more about this technology.
Left: Heatmap of cell density by region in mouse brain hemisphere stained with anti-c-FOS, generated with SmartAnalytics. Right: Histogram of cell density by region in same c-FOS stained hemisphere, generated by SmartAnalytics.
Although in the realm of neuroscience c-FOS protein is typically recognized as a marker of neuronal activity, it was originally discovered nearly forty years ago in rat fibroblasts and characterized as being encoded by a proto-oncogene (a gene that once mutated can become tumor-inducing) and
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