SmartSPIM is an advanced light sheet microscope capable of imaging large tissue samples with high speed and resolution. The system was designed from the ground up, with no limitation based on commercially available microscope bodies. The unique illumination arm of the SmartSPIM creates a flat-top illumination profile laterally, and a focused beam axially. To achieve homogeneous axial point spread functions (PSFz) across the entire FOV, the illumination optics scan the beam axially while synchronized to the camera’s rolling shutter detection.
Entire hemisphere was stained in 1 day with SmartLabel using SYTO 16 green fluorescent nucleic acid stain and imaged using SmartSPIM with a custom LifeCanvas 3.6x objective. Acquisition time was less than 1 hour. The high and uniform axial resolution allows for clear delineation of cell bodies in XY and importantly in Z.
Dynamically axially swept light sheet
Custom broadband chromatically corrected optics ( NA = 0.20 )
(higher NA illumination available as a custom item)
2 cm x 3.5 cm x 1.2 cm (standard)
3 cm x 5.5 cm x 1.2 cm (custom)
Infinity corrected (180 mm EFL tube lens)
3.6x = 1.8 µm/pix; 10x = 0.64 µm/pix; 15x = 0.41 µm/pix
3.6x: NA 0.2, PSFz = 3.2-4.0 µm
10x: NA 0.6, PSFz = 1.2-2.2 µm
15x: NA 0.4, PSFz = 1.4-2.4 µm
3.6x = 3650 µm ; 10x = 1300 µm; 15x = 850 µm
20 FPS during z-stack acquisition
(imaging speed can be reduced for increased resolution)
2048 x 2048 sCMOS with rolling shutter – synchronized to sweeping light sheet for confocal detection, high homogeneity, and improved resolution
Up to 6 in a single combiner (wavelengths from 488 – 785 nm)
SmartSPIM image of fluorescent protein expression in a subset of neurons. High-resolution axial spacing (4 µm) enables seamless display of original XY-dimensional data in orthogonal directions (XZ & YZ), providing for accurate identification of each cell body.
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