SeTau-647 Secondary Antibody

Ensuring superior photostability when labeling

Our SeTau-647 Secondary Antibody is the only commercially available pre-conjugated SeTau Secondary that will provide superior photostability when labeling. 

Features and highlights:

  • Most photostable far-red fluorescent dye we have tested.
  • Fast, uniform labeling: Combine with SmartBatch+ for the best results when staining with antibodies in the 647 channel. 
  • Applications: Ideal for light sheet, 2P Imaging, expansion tissues, super-resolution, or live cell imaging where fluorescence stability is paramount.
  • SeTau-647 secondary antibody is available in 0.5 mg vials. Each vial is preconjugated with SeTau-647 from SETA Biomedicals.

Video shows how SeTau-647 maintains photostability over time compared to a conventional 647 fluorophore. A whole mouse brain was labeled with anti-NeuN primary antibody. After hemisecting the brain, the hemisphere on the left was stained with a conventional 647 fluorophore secondary antibody, and the hemisphere on the right with SeTau-647. Hemispheres were glued together and imaged on SmartSPIM at 1.6X with 100% laser power.

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SeTau-647 versus a conventional fluorophore

Photobleaching occurs when a fluorophore loses its fluorescence due to light-induced damage. As different fluorophores have varied susceptibility to this phenomenon, we measure fluorescence intensity of different fluorophores over time to determine photostability.

LifeCanvas developed SeTau-647 Secondary Antibody which as observed in the graph, shows higher fluorescence intensity sustainability over time compared to conventional 647 fluorophores. This establishes SeTau-647 as the premier choice, showcasing superior photostability over conventional alternatives.

Minimizing photobleaching is crucial for light sheet imaging and other applications requiring fluorescence stability, such as 2P, expansion tissues, super-resolution, and live cell imaging.

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Documentation

Protocols

Recent publications citing SmartBatch+

Brain tumors induce widespread disruption of calvarial bone and alteration of skull marrow immune landscape

Abhishek Dubey, Erika Yamashita, Biljana Stangeland, Imane Abbas, David Fooksman, Robert A. Harris, Gregory M. Palmer, Wade R. Koba, Jinghang Zhang, Benjamin T. Himes, Olivia R. Lu, Winson S. Ho, Raoul V. Kuiper, Derek Huffman, Zhiping Wu, Yutaka Uchida, Masaru Ishii, Rachel L. Welch, Alexander F. Fiedler, David Reynolds, S. A. Mohieb Hosainey, Kostantin Dobrenis, Qinge Ye, Kevin Fisher, Nathaniel Killian, E. Richard Stanley, Emad Eskandar & Jinan Behnan

Journal:
Nature Neuroscience
Publication Date:
Research Area:
Cancer
Tissue Species:
Mouse
Tissue Type:
Brain, Bone
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM
Three-dimensional quantification of oxytocin neurons in the hypothalamic paraventricular nucleus reveals sex- and subregion-specific differences in two genetic mouse models of autism

Aishwarya Patwardhan, Siyao Li, Jessica Chen, Katrina Y. Choe

Journal:
Journal of Neuroendocrinology
Publication Date:
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM
Passive intestinal microbiome sampling using an ingestible device with tortuous lattices

Hanan Mohammed, Sadaf Usmani, Brij Bhushan, Anique Ahmad, Oraib Al-Ketan, Ahmed A. Shibl, Maylis Boitet, Devjoy Dev, Heba Naser, Aashish R. Jha, Khalil B. Ramadi

Journal:
Cell Device
Publication Date:
Research Area:
Methods, Other
Tissue Species:
Mouse
Tissue Type:
GI Tract
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, CRO Services
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Further reading

LifeCanvas Portraits: Dr. Mike Taormina

Dr. Mike Taormina uses SmartSPIM light sheet microscopy to rapidly image whole mouse brains, streamlining workflows at the Allen Institute.

For technical support reach out to our dedicated customer support team at support@lifecanvastech.com.