EasyIndex

Whole mouse brain before delipidation, after delipidation, and after optical clearing including refractive index matching

Refractive index matching, simplified

Delipidation and refractive index (RI) -matching allow light to pass unhindered through the sample during microscopy. In effect, tissue clearing refers to the combined process of delipidation and optical clearing via index matching.

Delipidated tissues will appear translucent, while cleared and RI-matched tissues will appear transparent. Our preformulated EasyIndex solution prepares samples for sharp, informative volumetric imaging with light sheet microscopes like SmartSPIM and MegaSPIM.

Tissue Clearing - Highlighted pipeline icon
EasyIndex bottle

Optically clear intact tissues and sections

The 500-mL EasyIndex bottle can be used for up to 12 whole mouse brains or similarly sized intact samples, or ~200 1 mm-thick sections. We provide RI = 1.52 standard solutions with RI = 1.46 solution available on request.

EasyIndex is ideal for index-matching multiple tissue types, immunolabeled tissues, and specimens cleared with aqueous methods like SHIELD or CLARITY.

Recent publications citing EasyIndex

Brain tumors induce widespread disruption of calvarial bone and alteration of skull marrow immune landscape

Abhishek Dubey, Erika Yamashita, Biljana Stangeland, Imane Abbas, David Fooksman, Robert A. Harris, Gregory M. Palmer, Wade R. Koba, Jinghang Zhang, Benjamin T. Himes, Olivia R. Lu, Winson S. Ho, Raoul V. Kuiper, Derek Huffman, Zhiping Wu, Yutaka Uchida, Masaru Ishii, Rachel L. Welch, Alexander F. Fiedler, David Reynolds, S. A. Mohieb Hosainey, Kostantin Dobrenis, Qinge Ye, Kevin Fisher, Nathaniel Killian, E. Richard Stanley, Emad Eskandar & Jinan Behnan

Journal:
Nature Neuroscience
Publication Date:
Research Area:
Cancer
Tissue Species:
Mouse
Tissue Type:
Brain, Bone
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM
Three-dimensional quantification of oxytocin neurons in the hypothalamic paraventricular nucleus reveals sex- and subregion-specific differences in two genetic mouse models of autism

Aishwarya Patwardhan, Siyao Li, Jessica Chen, Katrina Y. Choe

Journal:
Journal of Neuroendocrinology
Publication Date:
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM
Passive intestinal microbiome sampling using an ingestible device with tortuous lattices

Hanan Mohammed, Sadaf Usmani, Brij Bhushan, Anique Ahmad, Oraib Al-Ketan, Ahmed A. Shibl, Maylis Boitet, Devjoy Dev, Heba Naser, Aashish R. Jha, Khalil B. Ramadi

Journal:
Cell Device
Publication Date:
Research Area:
Methods, Other
Tissue Species:
Mouse
Tissue Type:
GI Tract
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, CRO Services

Further reading