Here, we demonstrate how the Megatome Premium can easily facilitate this process through whole-mount and non-destructive tissue sectioning.
Delipidation and refractive index (RI) -matching allow light to pass unhindered through the sample during microscopy. In effect, tissue clearing refers to the combined process of delipidation and optical clearing via index matching.
Delipidated tissues will appear translucent, while cleared and RI-matched tissues will appear transparent. Our preformulated EasyIndex solution prepares samples for sharp, informative volumetric imaging with light sheet microscopes like SmartSPIM and MegaSPIM.
The 500-mL EasyIndex bottle can be used for up to 12 whole mouse brains or similarly sized intact samples, or ~200 1 mm-thick sections. We provide RI = 1.52 standard solutions with RI = 1.46 solution available on request.
EasyIndex is ideal for index-matching multiple tissue types, immunolabeled tissues, and specimens cleared with aqueous methods like SHIELD or CLARITY.
Here, we demonstrate how the Megatome Premium can easily facilitate this process through whole-mount and non-destructive tissue sectioning.
Immunohistochemistry (IHC) and 3D histology are different approaches to detecting specific proteins in biological tissue.
Tissue clearing techniques have long since sought to maximize optical transparency while preserving sample architecture and minimizing processing time. While...
LifeCanvas tissue clearing is fast, reliable, and cost-effective, allowing uniform and simultaneous clearing for multiple samples.
For technical support reach out to our dedicated customer support team at support@lifecanvastech.com.