Tatiyana Adkins is the lead author on a recent JNeurosci paper uncovering the neural circuitry behind alcohol drinking and aversive...
The SmartSPIM light sheet microscope captures high quality 3D data from a variety of intact cleared tissues, from whole mouse and rat brains to marmoset brain hemispheres and even entire juvenile mice.
Unlike many other light sheet microscopes, which are limited by acquisition time and imaging chamber size, SmartSPIM combines an innovative acquisition technique with a modular design to make cleared tissue imaging easy, fast, and flexible. Intuitive software and expert support makes light sheet microscopy accessible for your whole lab, not just one specialist.
The SmartSPIM light sheet microscope leverages patented axial sweeping technology (Dean et al. 2015): illumination optics scan the beam while synchronized to the camera’s rolling shutter detection, providing homogeneous axial point spread functions (PSFz) across the entire field of view (FOV).
Left: The sample imaged without axial sweeping displays high axial resolution only at the center of the FOV, while the sample imaged with sweeping shows uniform axial resolution across the FOV.
Microglia (IBA-1, 647 nm) in cortex of a 7-month-old wild-type C57BL/6 male mouse from Taconic Biosciences, model #B6, 3.6X objective.
Custom-designed imaging chamber and sample holders can accommodate a variety of tissue sizes, from organoids to rat brains, and even entire juvenile mice. With no points of contact between tissue and mounting hardware, the user can mount many sample types and sizes in different orientations.
Tunable imaging parameters allow for high speed volumetric imaging while maintaining quality resolution. 20 fps acquisition allows imaging of a mouse brain hemisphere or comparably sized intact sample in <30 minutes (at 1.8 µm x 1.8 µm x 4 µm voxel size).
Streamlined for efficient data collection, SmartSPIM’s acquisition software has real-time tile correction and is simple to operate, making imaging large samples a breeze.
Tatiyana Adkins is the lead author on a recent JNeurosci paper uncovering the neural circuitry behind alcohol drinking and aversive...
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Dr. Sam Centanni uses the LifeCanvas pipeline to investigate the neurobiological overlap between stress and predisposition to alcohol abuse, as...
Dr. Mike Taormina uses SmartSPIM light sheet microscopy to rapidly image whole mouse brains, streamlining workflows at the Allen Institute.
Clear and label whole tissues while preserving key biomolecular information.
Acquire spatially informative images with innovative microscopy techniques.
Generate publication-quality data with robust analysis tools.
Tatiyana Adkins is the lead author on a recent JNeurosci paper uncovering the neural circuitry behind alcohol drinking and aversive...
We caught up with Dr. Bedenbaugh about her experience as a new investigator, what starting a new lab entails, and...
Dr. Montero Llopis shares her insights on the MicRoN Core’s vision, its adoption of LifeCanvas technologies, and how our values...
Dr. Tanya Daigle used LifeCanvas' services to acquire 3D images of spinal motor neurons illuminated by enhancer AAV.
