Contract Research Services

We are now offering service as a way for members of the research community to:

1. Evaluate the potential of adopting cleared tissue / 3D whole organ approaches into their research program on a complimentary basis (first trial of our contract research services is free with your own sample) 

 

2. Take advantage of our  whole-organ processing and imaging expertise that we’ve developed in-house by contracting with us to design and/or conduct studies on your behalf to get 3D data

Our Services

Experimental Design Consultation

Before beginning your study, we will work with you to understand the strengths of our tissue-processing pipeline, how best to prepare your samples, what results to expect, and other key details critical to project success.

 

SHIELD Preservation

To process your samples, we first use a novel, polyepoxide-based fixation technique called SHIELD (Park et al, Nature Biotech, 2018) to maximally preserve the tissue’s endogenous biomolecules, including fluorescent proteins and targets for immunohistochemical labeling.

 

Tissue Clearing

We then use detergents and electrophoresis (via SmartClear 2 Pro) to optically clear the tissue by removing lipid-filled cell membranes, which hinder antibody penetration and limit imaging depth by causing light to scatter.

 

Active Labeling

Using SmartLabel, which incorporates our proprietary stochastic electrotransport technology (Kim et al, PNAS, 2015), we then label intact samples like mouse brain hemispheres with molecular probes such as IgG antibodies in just 1-3 days.

 

Refractive Index-Matching and Sample Embedding:

After clearing (and optional labeling), we turn translucent, delipidated samples fully transparent by incubating them in a solution (EasyIndex) that raises and homogenizes their refractive index, greatly enhancing penetration of the visible wavelengths of light. The sample is then mounted in an agarose gel for maximum stability during imaging, aiding colocalization of signals across channels.

 

Light-Sheet Imaging:

Intact samples are then imaged in their entirety using our light-sheet microscope, SmartSPIM, generating datasets at a default voxel size (1.8 µm x 1.8 µm x 4 µm). Other acquisition parameters can be discussed in our initial consultation.

 

Data Analysis (i.e. cell counting)

Using image volumes sufficient to identify each cell in 3D (e.g., using tissue injected with viral tracers, or following anti-cFos staining using SmartLabel), we can then quantify the labeling pattern in a number of ways to meet your needs.

 

Sign up for Free Trial of Sample Processing Service

Service Information Form (click above to download)

Please share details of your tissue samples and research goals and we will follow-up with you soon!