(2) Figure 6. Light-sheet imaging of tyrosine hydroxylase (TH)+ axon terminals (red; middle left) together with Iba1-labeled microglia (green; left) confirmed their spatial proximity. Middle right, three-dimensional (3D) rendering image of a 50-μm-thick slice (scale bar, 20 μm); top right, high-magnification view of the boxed region (scale bar, 5 μm); bottom right, further enlarged view of the region in the dashed box and automatically detected axon boutons (magenta) (scale bar, 2 μm). b, Distance of boutons to the nearest microglia. Article licensed under the terms of the http://creativecommons.org/licenses/by/4.0/.
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