Mouse Brain: Rabbit Melanin Concentrating Hormone
Antibody link: abcam ab274415
Rabbit Melanin concentrating hormone staining in a whole mouse brain (40 µm max intensity projection).
Zoom in of hypothalamus/thalamus (left) and hindbrain (right) below.
Protocol
1. Standard SHIELD protocol.
2. Passive delipidation with Clear+ Delipidation Buffer.
3. 2 days blocking with Blocking Buffer at 37°C.
4. Radiant Labeling in SmartBatch+ using 6 µg Rabbit Anti-MCH antibody in the Single Sample Staining Cup.
5. Secondary Labeling with 12 µg Donkey anti-Rabbit Alexa 555+ in SmartBatch+.
6. EasyIndex index matching and mounting.
7. Imaged on SmartSPIM with 3.6X objective.