Mouse Brain: Rabbit CNP
Antibody link: Encor RPCA-CNP
Rabbit CNP staining in a whole mouse brain (40 µm max intensity projection).
Zoom in of cortex / corpus collosum / hippocampus (left) and cerebellum (right) below.
Protocol
1. Standard SHIELD protocol.
2. Passive delipidation with Clear+ Delipidation Buffer.
3. 2 days blocking with Blocking Buffer at 37°C.
4. Radiant Labeling in SmartBatch+ using 20 µg Rabbit Anti-CNP antibody in the Single Sample Staining Cup.
5. Secondary Labeling with 40 µg Donkey anti-Rabbit SeTau-647 in SmartBatch+.
6. EasyIndex index matching and mounting.
7. Imaged on SmartSPIM with 3.6X objective.