Tissue Clearing & Labeling

We now offer active tissue clearing and antibody labeling in one high-throughput device! SmartBatch+ switches between clearing and labeling modes simply by changing buffers, and processes 12 whole mouse brains or comparably sized samples per batch. Leveraging our new Clear+ tissue clearing method, eFLASH, and patented stochastic electrotransport technology, SmartBatch+ rapidly clears and uniformly labels whole organs with no change in tissue morphology and no surface saturation of antibodies or dyes.

Our legacy active tissue clearing system employs a patented stochastic electrotransport mechanism to foster rapid delivery of detergent into tissues, facilitating uniform removal of light-scattering membrane lipids. SmartClear ensures homogenous delipidation while preventing black particle formation, all with economic and user-friendly Clear+ buffers that last up to 10 days.

By combining our patented stochastic electrotransport method with SWITCH, our legacy active immunolabeling system achieves uniform whole-organ antibody labeling from surface to core. Compared to passive labeling techniques such as iDISCO and CUBIC, SmartLabel provides superior penetration and uniformity of probes in just 1-2 days, allowing you to visualize proteins deep within an organ and study the fine-scale topography of cells.

Light Sheet Microscopy

With its axially-swept light sheet design, our SmartSPIM microscope provides unprecedented resolution and uniformity over a large field of view. SmartSPIM is optimized to quickly image large, cleared tissue samples, featuring a large imaging chamber, easy-to-use interface, and rapid volumetric acquisition rates.

SmartSPIM can image at magnifications from 1.63x to 22x, and easily accommodates samples up to 40mm x 75mm. Continuous focus throughout the thickness of the samples ensures quality data throughout the imaging volume, and intuitive software allows for faster data acquisition with less effort.

Quantify your results using LifeCanvas’ SmartSPIM-optimized workstation. LifeCanvas performs cell detection across the entire sample using machine learning pipelines customized and optimized for 3D fluorescent microscopy images. Generate detailed analysis outputs: visualize your effects with our publication-quality heatmaps, and statistically verify your experimental questions with per-brain region cell counts and densities for each sample.