Delineate Tissue Architecture and Cell Mass

Tissue Mapping

Use Case: Pinpoint the spatial location of specific cell types within tissues and organoids

  • Mark tissue boundaries: Utilize various cell-type and structural markers to delineate biological regions with disparate makeup, such as cortical layers in the brain or putative cortical layers in organoids.
  • Segment cell masses: Utilize three dimensional imaging data to precisely demarcate the border of cell masses such as tumors and islets cells growing within tissues.
  • Define unique morphological features: Identify unique morphological features such as capsules, fissures, or crypts within complex tissues.

Extended Figure 2E (Ref 2): 3D delineation of the mouse primary motor cortex layer (MOp) borders based on light sheet imaging of whole brain immunostained with anti-NeuN and anti-Neurofilament-M. (Top) An optical section (left) and zoom-in view (right). (Bottom) An optical section of the entire brain and 3D rendering of MOp (left) and the cortical layer borders of MOp delineated based on autofluorescence (black dotted lines) and immunofluorescence (white dotted lines)(right). Article licensed under the terms of the Creative Commons CC-BY.

For additional publications supporting this use case refer to:

  1. Albanese et al. Scientific Reports 10, 21487 (2020) – Figure 2i, 2l, 3b, 3c, 3f-h, 5, Supplementary Video 1-4
  2. Muñoz-Castañeda et al. Nature 598, 159–166 (2021) – Extended Figure 2e
  3. Shah et al. Journal of Experimental Medicine 220 (2): e20220618 (2023) – Figure 2h, 3f, Video 1-3
  4. Von Roemeling et al. Nature Communications 15, 5871 (2024) – Supplementary Figure 5h
  5. Rosselot et al. Science Translational Medicine 16(755):eadg3456 (2024)
Our experienced team of application scientists have protocols to get you to data quickly with validated applications and can propose customized solutions for you for unvalidated applications. Fill the form below to get in touch with someone from the applications team today.
Contact Us

For technical support reach out to our dedicated customer support team at support@lifecanvastech.com.