Discover anterograde and retrograde connections

Circuit Mapping

Use Case: Discover anterograde and retrograde connections

  • Delineate connections: Label mono-synaptically connected neurons using anterograde and retrograde viruses and comprehensively map them in brain atlas space at single cell resolution. 
  • Localize projections: Identify dense projections that map to local and distant brain regions, revealing potential new circuits for further anatomical and functional investigation.
  • Identify connected neurons: Molecularly profile traced neurons using brain-wide immunolabeling of celltype defining markers and colocalization analysis.

Figure 6 (Ref 1): (a) Diagram of the viral strategy for tracing the control network for insula→BNST cells. (b) Schematic of rabies tracing strategy. (c) Maximum projection image of 3D reconstruction of light sheet data set to illustrate insula→BNST control network. (d) Resampled images in the coronal plane at the level of the anterior insula. (e) At the level of the BNST. (f) At the level of thalamic and amygdalar structures. (g) Distribution of labeled neurons represented as a composite whole-brain density heatmap of semi-autonomously quantified insula→BNST control network cells as density in mm3. (h) Graphical representation of cell densities in top eight regions (by raw cell count) labeled as part of the labeled insula→BNST control network of five mice. (i) Top eight regions (by cell count per region/total cells in each respective brain × 100) of the labeled insula→BNST control network. (j) Top eight regions (by cell density) of the labeled insula→BNST control network. Article is licensed under the Creative Commons Attribution 4.0 International License.

For additional publications supporting this use case refer to:

  1. Luchsinger et al. Nature Communications 12(1), 3561 (2021) – See Figures 6, 7, 8a-8g, supplementary movie 1-12
  2. Ghosh et al. Nature Neuroscience 25, 390–398 (2022) – See Figure 4a-4b
  3. Brown et al. Neuropsychopharmacology 48, 1133–1143 (2023) – See Figure 5a-5f
  4. Williford et al. Neuropsychopharmacology 48, 1031–1041 (2023) – See Figure 3a-3c, Supplementary Figure 3
  5. Escobedo et al. (2023) Elife 12, RP90972 (2023) – See Figure 1a, Supplementary Figure 2m
  6. Antonoudiou et al. Cell Reports 43(7), 114489 (2024) – See Supplementary Figure 4f-4g
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