DALISPIM is an open-top, inverted light sheet microscope that combines high-speed, high-resolution volumetric imaging with broad sample compatibility. Using patent-pending Depth Adaptive Light Interface (DALI) technology, it enables seamless, scalable imaging of diverse tissue types in standard sample carriers like multiwell plates.
In a typical inverted light-sheet setup, the light paths must be oblique to the surface of the sample carrier, which can result in refractive index (RI) mismatches and optical aberrations.
DALISPIM avoids this by pairing an enclosed RI-matching immersion with a deformable interface (see right), creating an RI-matched light path throughout the z-depth range. This allows DALISPIM to achieve optical coupling with standard sample holders – no immersion baths necessary.
Now you can effortlessly acquire high-quality 3D data from samples in standard holders like petri dishes, well plates, slide glasses, and organ-on-a-chip devices – no immersion bath necessary.
DALISPIM is compatible with live or cleared tissues including intact organs, organoids, biopsies, sections, and even entire model organisms. Its flexible configuration accomodates laterally large plates up to 125 x 85 mm, or custom containers up to 250 x 75 x 6 mm.
DALISPIM captures data with near isotropic resolution at imaging speeds 1-2 orders of magnitude faster than conventional confocal microscopy. Custom-designed optics and patented axial sweeping technology (Dean et al. 2015) deliver exceptional image quality for large-scale imaging.
Axial sweeping allows DALISPIM to generate images with uniformly high resolution in all axes without compromising acquisition speed. Illumination optics scan the beam while synchronized to the camera’s rolling shutter detection, providing homogeneous axial point spread functions (PSFz) across the entire field of view.
Imaging setup and acquisition: Our user-friendly software allows you to easily set up boundary parameters, optimize acquisition settings, and navigate through your sample(s), with step-by-step instructions for alignment and tiling.
Post-processing: DALISPIM comes with a comprehensive post-processing package to seamlessly take you from raw data to volumetric datasets.
Data storage: We now offer MOSAIC – a high-performance, networked storage solution optimized for light-sheet datasets. Learn more about MOSAIC here.
| Technical specifications | |
|---|---|
|
Light sheet formation |
Dynamic axial sweeping (Dean et al. 2015) |
|
Lateral specimen size |
Up to 250 x 75 mm stage travel |
|
Illumination objective |
Custom 0.125 NA, 25 mm WD |
|
Detection objectives |
3.6X, 9X |
|
Camera |
Hamamatsu Orca Fusion sCMOS |
|
Laser lines |
405 – 785 nm, up to 7 lines |
|
Refractive index compatibility |
1.33 – 1.56 (all objectives) |
|
Multi-sample imaging |
Automated sequential and array imaging |
Prepare samples with tools for preservation, clearing, labeling, & sectioning.
Acquire spatially informative images with innovative microscopy techniques.
Generate publication-quality data with robust analysis tools.
For technical support reach out to our dedicated customer support team at support@lifecanvastech.com.
