Extended Figure 2E (Ref 2): 3D delineation of the mouse primary motor cortex layer (MOp) borders based on light sheet imaging of whole brain immunostained with anti-NeuN and anti-Neurofilament-M. (Top) An optical section (left) and zoom-in view (right). (Bottom) An optical section of the entire brain and 3D rendering of MOp (left) and the cortical layer borders of MOp delineated based on autofluorescence (black dotted lines) and immunofluorescence (white dotted lines)(right). Article licensed under the terms of the Creative Commons CC-BY.
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