Circuit Mapping | Trace Local and Long-Range projections

Circuit Mapping

Use Case: Trace Local and Long-Range Projections

  • Map connected brain regions: Identify neuronal projections from a brain region and neuronal inputs to a brain region from every atlas defined region using anterograde and retrograde virus tracing without having to section the brain.
  • Reconstruct entire neurons: Make accurate reconstructions of elaborate axonal and dendritic arbors that make fine connections with local and distant regions of the central nervous system to make inferences about how signals are processed in the brain.
  • Make complete wiring diagrams: Create connectomes or detailed structural maps of connectivity to investigate how mis-wiring of these connections can lead to dysfunction in conditions ranging from epilepsy and autism to schizophrenia and Alzheimer’s.

(Ref 1) Rabies-labeled, SHIELD-cleared brain with full SNr neuronal reconstructions reveal that their dendrites spread partway into adjacent rostral and caudal levels, but do not span the entire length of SNr.

Article licensed under the terms of the http://creativecommons.org/licenses/by/4.0/.

For additional publications supporting this use case refer to:

  1. Foster et al. Nature 598, 188–194 (2021) – Figure 1k
  2. Bedenbaugh et al. (2022) Journal of Comparative Neurology 530(16):2835-2851 – Figure 6, Movie 1-5
  3. Chen et al. Cell Reports 43(3):113829 (2024) – Figures 1a-1c, 2a-2f, 6a
  4. Park et al. Science 384(6701):eadh9979 (2024) – Figure 2l-2m, 8a-8f
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