Perfusion
Before SHIELD begins, the animal should be perfused to remove residual blood, which can cause issues with autofluorescence or nonspecific antibody labeling. Proper technique is essential for generating good data.
This protocol outlines a standard perfusion procedure to remove blood from the tissue of interest.
Use this protocol if you wish to perfuse your sample with the SHIELD solutions as seen in the publication by Park et al..
We have seen better performance using the Standard Protocol, which also requires less reagents, compared to this protocol.