Cell Mapping | Pinpoint the spatial location of specific cell types within tissues and organoids

Use Case: Pinpoint the spatial location of specific cell types within tissues and organoids

Map cells precisely in 3D: Use viral, transgenic or immunolabeling of cell-specific markers to readily identify distinct cell-types within organ and organ-like tissue environments.

Uncover anatomical insights: Use quantitative analysis on spatial positions of single cells, vessels, and tissue borders to measure multi-scale features that characterize the microenvironment, cytoarchitecture, and whole tissue.

Monitor pathophysiological progression: Capture the cellular response to diseased, damaged, and inflamed tissues using immune cell-specific markers.

A 3D rendering of a D59 (FWK8) human fetal eye after whole mount immunostaining and clearing. Recoverin (RCVRN, magenta) labeling is only restricted to one specific region of the retina, temporal to the optic nerve. IBA1 (green) was used to visualize the structure of the retina.

Video S1, extracted from Wohlschlegel, J., et al. ASCL1 induces neurogenesis in human Müller glia. Stem Cell Reports, 18(12), 2400-2417 (2023). https://doi.org/10.1016/j.stemcr.2023.08.004. Article licensed under the terms of the Creative Commons CC-BY.

For additional publications supporting this use case refer to:

Folorunso et al. Sci Rep 13, 9595 (2023) – See Supplementary Figure 2
Wholschlegel et al. Stem Cell Reports 18(12):P2400-2417 (2023) – See Figure 1D, Video S1
Nudell et al. Nature Methods 19, 479–485 (2022) – See Figure 3, 4g-4q, 5e-5k, Supplementary Videos 1-4
Bedenbaugh et al. Journal of Comparative Neurology 530(16): 2835–2851 (2022) – See Figure 2, 6, Supplementary movies 1-5
Paulsen et al. Nature 602, 268–273 (2022) – See Extended Data 4a-4b
Sweeney et al. Science Translational Medicine 13(590): eabd6434 (2021) – See Figure 1a-1d, Movie S1-S4
Knights et al. EMBO Journal 40: e106061 (2021) – See Figure 1a, Extended View Figure 1a
Albanese et al. Scientific Reports 10, 21487 (2020) – See Figure 1e, 5h, 6a, Supplementary Videos 1-4

Our experienced team of application scientists have protocols to get you to data quickly with validated applications and can propose customized solutions for you for unvalidated applications. Fill the form below to get in touch with someone from the applications team today.

Cell Mapping | Pinpoint the spatial location of specific cell types within tissues and organoids

Use Case: Pinpoint the spatial location of specific cell types within tissues and organoids

Map cells precisely in 3D: Use viral, transgenic or immunolabeling of cell-specific markers to readily identify distinct cell-types within organ and organ-like tissue environments.

Uncover anatomical insights: Use quantitative analysis on spatial positions of single cells, vessels, and tissue borders to measure multi-scale features that characterize the microenvironment, cytoarchitecture, and whole tissue.

Monitor pathophysiological progression: Capture the cellular response to diseased, damaged, and inflamed tissues using immune cell-specific markers.

Our experienced team of application scientists have protocols to get you to data quickly with validated applications and can propose customized solutions for you for unvalidated applications. Fill the form below to get in touch with someone from the applications team today.

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