Tissue Clearing & Labeling

Delipidation and conduction buffers for tissue clearing

Our new Clear+ tissue clearing technique is the only method that delipidates samples with no change in morphology and with minimal impact on structural integrity. Fully delipidate whole mouse brains or comparably sized samples in just one day with SmartBatch+, or in one week with our passive clearing kit.

SmartBatch+

Our turn-key SmartBatch+ system combines electrophoretic tissue clearing and immunolabeling into one high-throughput device. Leverage the Clear+ tissue clearing method, along with eFLASH and patented stochastic electrotransport technologies, to rapidly clear and label whole organs. Key highlights and features include:

  • Batch processing of up to 12 whole mouse brains, 4 whole rat brains, or comparably sized samples
  • Uniform labeling with no surface saturation of antibodies or dyes, and minimal antibody usage
  • No immunolabeling necessary for endogenous fluorescence (no fluorescent protein quenching)
  • 1-day active tissue clearing, 2-day active immunolabeling (primary & secondary)

Our SeTau-647 Secondary Antibody is the only commercially available pre-conjugated SeTau Secondary that will provide superior photostability when labeling. Ideal for light sheet, 2P Imaging, expansion tissues, super-resolution, or live cell imaging where fluorescence stability is paramount.

Specifications:

  • Antibody Format: Whole IgG
  • Clonality: Polyclonal
  • Host: Donkey
  • Minimal Cross Reactivity: Bovine, chicken, goat, guinea pig, syrian hamster, horse, human, mouse, rat, sheep serum proteins (except for reactive target)
  • Physical State: Lyophilized solid
  • Provided in 0.5 mg vials.
EasyIndex for Index Matching

Our preformulated EasyIndex solution raises and homogenize the refractive index of delipidated tissue samples, rendering them fully transparent. This enables light penetration into the sample and ensures the acquisition of high-resolution, in-focus image data.

Recent publications citing LifeCanvas tissue clearing & labeling techniques

Anatomical and molecular organization of the nociceptive medial thalamus

Lindsay L. Ejoh, Raquel Adaia Sandoval Ortega, Jacqueline W. K. Wu, Alaa Elhiraika, Malaika Mahmood, Kyungdong Kim, Emmy Li, Oliver Joseph, Lisa M. Wooldridge, Jessica A. Wojick, Gregory J. Salimando, Gregory Corder

Journal:
biorXiv
Publication Date:
Research Area:
Neural Activity, Neural Circuits
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, CRO Services
Brain-immune interactions generate pathogen-specific sickness states

Zuri A Sullivan, Bertrand J. Wong, Vikrant Kapoor, Paula Kathleen Vincze, Harris S. Kaplan, Pia Giraudet, Brianna R. Watson, Adel Misherghi, Sebastian Lourido, Jeffrey R Moffitt, Catherine Dulac

Journal:
biorXiv
Publication Date:
Research Area:
Neural Activity
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, SmartAnalytics, CRO Services
Psilocybin triggers an activity-dependent rewiring of large-scale cortical networks

Quan Jiang, Ling-Xiao Shao, Shenqin Yao, Neil K. Savalia, Amelia D. Gilbert, Pasha A. Davoudian, Jack D. Nothnagel, Guilian Tian5, Tin Shing Hung, Hei Ming Lai, Kevin T. Beier, Hongkui Zeng, Alex C. Kwan

Journal:
Cell
Publication Date:
Research Area:
Neural Activity
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, SmartAnalytics, CRO Services