Tissue Clearing & Labeling

Delipidation and conduction buffers for tissue clearing

Our new Clear+ tissue clearing technique is the only method that delipidates samples with no change in morphology and with minimal impact on structural integrity. Fully delipidate whole mouse brains or comparably sized samples in just one day with SmartBatch+, or in one week with our passive clearing kit.

SmartBatch+

Our turn-key SmartBatch+ system combines electrophoretic tissue clearing and immunolabeling into one high-throughput device. Leverage the Clear+ tissue clearing method, along with eFLASH and patented stochastic electrotransport technologies, to rapidly clear and label whole organs. Key highlights and features include:

  • Batch processing of up to 12 whole mouse brains, 4 whole rat brains, or comparably sized samples
  • Uniform labeling with no surface saturation of antibodies or dyes, and minimal antibody usage
  • No immunolabeling necessary for endogenous fluorescence (no fluorescent protein quenching)
  • 1-day active tissue clearing, 2-day active immunolabeling (primary & secondary)

Our SeTau-647 Secondary Antibody is the only commercially available pre-conjugated SeTau Secondary that will provide superior photostability when labeling. Ideal for light sheet, 2P Imaging, expansion tissues, super-resolution, or live cell imaging where fluorescence stability is paramount.

Specifications:

  • Antibody Format: Whole IgG
  • Clonality: Polyclonal
  • Host: Donkey
  • Minimal Cross Reactivity: Bovine, chicken, goat, guinea pig, syrian hamster, horse, human, mouse, rat, sheep serum proteins (except for reactive target)
  • Physical State: Lyophilized solid
  • Provided in 0.5 mg vials.
EasyIndex for Index Matching

Our preformulated EasyIndex solution raises and homogenize the refractive index of delipidated tissue samples, rendering them fully transparent. This enables light penetration into the sample and ensures the acquisition of high-resolution, in-focus image data.

Recent publications citing LifeCanvas tissue clearing & labeling techniques

Brain tumors induce widespread disruption of calvarial bone and alteration of skull marrow immune landscape

Abhishek Dubey, Erika Yamashita, Biljana Stangeland, Imane Abbas, David Fooksman, Robert A. Harris, Gregory M. Palmer, Wade R. Koba, Jinghang Zhang, Benjamin T. Himes, Olivia R. Lu, Winson S. Ho, Raoul V. Kuiper, Derek Huffman, Zhiping Wu, Yutaka Uchida, Masaru Ishii, Rachel L. Welch, Alexander F. Fiedler, David Reynolds, S. A. Mohieb Hosainey, Kostantin Dobrenis, Qinge Ye, Kevin Fisher, Nathaniel Killian, E. Richard Stanley, Emad Eskandar & Jinan Behnan

Journal:
Nature Neuroscience
Publication Date:
Research Area:
Cancer
Tissue Species:
Mouse
Tissue Type:
Brain, Bone
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM
Three-dimensional quantification of oxytocin neurons in the hypothalamic paraventricular nucleus reveals sex- and subregion-specific differences in two genetic mouse models of autism

Aishwarya Patwardhan, Siyao Li, Jessica Chen, Katrina Y. Choe

Journal:
Journal of Neuroendocrinology
Publication Date:
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM
Passive intestinal microbiome sampling using an ingestible device with tortuous lattices

Hanan Mohammed, Sadaf Usmani, Brij Bhushan, Anique Ahmad, Oraib Al-Ketan, Ahmed A. Shibl, Maylis Boitet, Devjoy Dev, Heba Naser, Aashish R. Jha, Khalil B. Ramadi

Journal:
Cell Device
Publication Date:
Research Area:
Methods, Other
Tissue Species:
Mouse
Tissue Type:
GI Tract
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, CRO Services