Tissue Clearing & Labeling

Delipidation and conduction buffers for tissue clearing

Our new Clear+ tissue clearing technique is the only method that delipidates samples with no change in morphology and with minimal impact on structural integrity. Fully delipidate whole mouse brains or comparably sized samples in just one day with SmartBatch+, or in one week with our passive clearing kit.

A black machine with a digital control panel displaying blue screens and buttons. "LifeCanvas Technologies" logo on the front.

Our turn-key SmartBatch+ system combines electrophoretic tissue clearing and immunolabeling into one high-throughput device. Leverage the Clear+ tissue clearing method, along with eFLASH and patented stochastic electrotransport technologies, to rapidly clear and label whole organs. Key highlights and features include:

  • Batch processing of up to 12 whole mouse brains, 4 whole rat brains, or comparably sized samples
  • Uniform labeling with no surface saturation of antibodies or dyes, and minimal antibody usage
  • No immunolabeling necessary for endogenous fluorescence (no fluorescent protein quenching)
  • 1-day active tissue clearing, 2-day active immunolabeling (primary & secondary)

Our SeTau-647 Secondary Antibody is the only commercially available pre-conjugated SeTau Secondary that will provide superior photostability when labeling. Ideal for light sheet, 2P Imaging, expansion tissues, super-resolution, or live cell imaging where fluorescence stability is paramount.

Specifications:

  • Antibody Format: Whole IgG
  • Clonality: Polyclonal
  • Host: Donkey
  • Minimal Cross Reactivity: Bovine, chicken, goat, guinea pig, syrian hamster, horse, human, mouse, rat, sheep serum proteins (except for reactive target)
  • Physical State: Lyophilized solid
  • Provided in 0.5 mg vials.
EasyIndex for Index Matching

Our preformulated EasyIndex solution raises and homogenize the refractive index of delipidated tissue samples, rendering them fully transparent. This enables light penetration into the sample and ensures the acquisition of high-resolution, in-focus image data.

Recent publications citing LifeCanvas tissue clearing & labeling techniques

The S1–insula Circuit Differentially Modulates Alcohol Drinking and Aversive Behavior in Mice

Tatiyana L. Adkins, Amanda L. Salazar, Jincy R. Little, Ellen C. Howard, Samuel W. Centanni

Journal:
Journal of Neuroscience
Publication Date:
Research Area:
Neural Circuits
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SmartBatch+, SmartSPIM
A human cardiomyocyte screen identifies optimized lipid nanoparticles for in vivo cardiac gene editing

Yehui Sun, Yu-Chung Pien, Yufen Xiao, Wei Tan, Efrain Sanchez-Ortiz, Mateusz Z. Durbacz, Zexiang Chen, John R. McAnally, Yu Zhang, Hui Li, Andreas C. Chai, Francesco Chemello, Fangyu Zhang, Sang M. Lee, Priyanka Patel, Denise M. Ramirez, Sumanta Chatterjee, Ning Liu, Eric N. Olson, Daniel J. Siegwart

Journal:
PNAS
Publication Date:
Research Area:
Cardiovascular, Biodistribution
Tissue Species:
Mouse
Tissue Type:
Heart
Products Cited:
SHIELD, EasyIndex, Passive Clearing Kit, SmartSPIM
The midbrain reticular formation in contextual control of perceptual decisions

Jordan R. Shaker, Jeremy N. Schroeter, Daniel Birman, Nicholas A. Steinmetz

Journal:
Neuron
Publication Date:
Research Area:
Neural Circuits
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, Passive Clearing Kit, SmartSPIM