Mouse Brain: Rabbit Parvalbumin
Antibody link: ThermoFisher PA1-933
Parvalbumin staining in a whole mouse brain (40 µm max intensity projection).
Zoom in of cortex (left) and cerebellum (right) below.
Protocol
1. Standard SHIELD protocol.
2. Passive delipidation with Clear+ Delipidation Buffer.
3. 2 days blocking with Blocking Buffer at 37°C.
4. Radiant Labeling in SmartBatch+ using 10 µg Rabbit anti-Parvalbumin antibody in the Single Sample Staining Cup.
5. Secondary Labeling with 20 µg Donkey anti-Rabbit Alexa 555+ in SmartBatch+.
6. EasyIndex index matching and mounting.
7. Imaged on SmartSPIM with 3.6X objective.