Proximity Mapping | Co-localize cells, cellular structures and protein aggregates

Proximity Mapping

Use Case: Co-localize cells, cellular structures, and protein aggregates

  • ID cells with cell-specific markers:
    • Identify cells of interest such as active neurons, invading immune cells, differentiated cells by colocalizing them with molecular markers that distinguish their cellular identity.
  • Define tissue microenvironments:
    • Delineate the local, 3D environment of cells, molecules, and structures that surround and support other cells by conducting a proximity analysis of the local signaling molecules, interacting cells, and structural components.
  • Examine the impact of proteinopatheis:
    • Gather evidence for the devastating effects of abnormal protein aggregation on the surrounding tissue microenvironments and the ensuing biological response by colocalizing these protein aggregates with other biological markers.
Figure 6: Light-sheet imaging showing TH+ axon terminals (red) near Iba1-labeled microglia (green). 3D slice rendering with 20 μm scale; high-magnification views at 5 μm and 2 μm show axon boutons (magenta). Graph of bouton-to-microglia distance

(1) Figure 6. Light-sheet imaging of tyrosine hydroxylase (TH)+ axon terminals (red; middle left) together with Iba1-labeled microglia (green; left) confirmed their spatial proximity. Middle right, three-dimensional (3D) rendering image of a 50-μm-thick slice (scale bar, 20 μm); top right, high-magnification view of the boxed region (scale bar, 5 μm); bottom right, further enlarged view of the region in the dashed box and automatically detected axon boutons (magenta) (scale bar, 2 μm). b, Distance of boutons to the nearest microglia. Article licensed under the terms of the http://creativecommons.org/licenses/by/4.0/.

For additional publications supporting this use case refer to:

  1. Chen et al. Proc Natl Acad Sci 120(21):e2220684120 (2023) – Figure 4a, 4b, Movie 1, 2
  2. Ma et al. (2024) Nature Communications 27, 249–258 (2024) – Figure 6a, 6b
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