Light Sheet Microscopy

DALISPIM

DALISPIM is an open-top, inverted light sheet microscope that combines high-speed, high-resolution volumetric imaging with broad sample compatibility. Key highlights and features include:

  • Patented axial sweeping technology, which generates homogenous PSFz across field of view
  • Aberration-free optical coupling with standard sample holders (e.g. well plates, slide glasses, petri dishes, organ-on-a-chip devices) without the need for immersion baths
  • Capacity for well plates up to 125 x 85 mm, or custom containers up to 250 x 75 –  image live or cleared tissues including intact organs, organoids, biopsies, sections, and even entire model organisms
  • Custom-designed optics and patented axial sweeping technology for rapid acquisition of images with uniformly high resolution in all axes
  • Easy-to-use setup and acquisition software
  • Comprehensive post-processing package
Learn more about DALISPIM
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SmartSPIM​

The SmartSPIM light sheet microscope provides unparalleled resolution and acquisition speed throughout intact tissue volumes, while its modular design allows you to upgrade and customize individual components.
Key highlights and features include:

  • Patented axial sweeping technology, which generates homogenous PSFz across field of view
  • Rapid volumetric acquisition (intact mouse brain hemisphere in <30 minutes)
  • Large imaging chamber and dual illumination, optimized for large (up to 40mm x 75mm) or multi-sample acquisition – easily image organoids, rat brains, entire juvenile mice, & everything in between
  • Focus compensation and tunable imaging parameters
  • Intuitive acquisition software with real-time tile correction
  • Objectives available for 1.63X through 22X magnification
Learn more about SmartSPIM
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MegaSPIM Light Sheet Microscope

MegaSPIM

The MegaSPIM microscope is our newest light sheet imaging system. Utilizing the same powerful axial sweeping technology as SmartSPIM, MegaSPIM is optimized for imaging not only laterally large samples, but thin slices and arrays of small tissues as well. Unique highlights and features include:

  • Capacity for lateral sample sizes up to 200 mm x 200 mm –  easily image expanded tissues, large slabs, and arrays of organoids, slices, or whole rodent organs
  • Custom mounting solutions for a variety of sample types
  • High-throughput multicolor acquisition with dual cameras and independent focus correction
  • Post-processing tools to deskew and stitch your data into complete ready-to-analyze volumes
Learn more about MegaSPIM
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Recent publications citing LifeCanvas light sheet microscopy

A parabrachial hub for need-state control of enduring pain

Nitsan Goldstein, Amadeus Maes, Heather N. Allen, Tyler S. Nelson, Kayla A. Kruger, Morgan Kindel, Albert T. M. Yeung, Nicholas K. Smith, Jamie R. E. Carty, Lavinia Boccia, Niklas Blank, Emily Lo, Rachael E. Villari, Ella Cho, Erin L. Marble, Michelle Awh, Yasmina Dumiaty, Melissa J. Chee, Rajesh Khanna, Christoph A. Thaiss, Bradley K. Taylor, Ann Kennedy & J. Nicholas Betley

Journal:
Nature
Publication Date:
Research Area:
Neural Circuits
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM, CRO Services
Brain tumors induce widespread disruption of calvarial bone and alteration of skull marrow immune landscape

Abhishek Dubey, Erika Yamashita, Biljana Stangeland, Imane Abbas, David Fooksman, Robert A. Harris, Gregory M. Palmer, Wade R. Koba, Jinghang Zhang, Benjamin T. Himes, Olivia R. Lu, Winson S. Ho, Raoul V. Kuiper, Derek Huffman, Zhiping Wu, Yutaka Uchida, Masaru Ishii, Rachel L. Welch, Alexander F. Fiedler, David Reynolds, S. A. Mohieb Hosainey, Kostantin Dobrenis, Qinge Ye, Kevin Fisher, Nathaniel Killian, E. Richard Stanley, Emad Eskandar & Jinan Behnan

Journal:
Nature Neuroscience
Publication Date:
Research Area:
Cancer
Tissue Species:
Mouse
Tissue Type:
Brain, Bone
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM
Three-dimensional quantification of oxytocin neurons in the hypothalamic paraventricular nucleus reveals sex- and subregion-specific differences in two genetic mouse models of autism

Aishwarya Patwardhan, Siyao Li, Jessica Chen, Katrina Y. Choe

Journal:
Journal of Neuroendocrinology
Publication Date:
Tissue Species:
Mouse
Tissue Type:
Brain
Products Cited:
SHIELD, EasyIndex, SmartBatch+, SmartSPIM
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For technical support reach out to our dedicated customer support team at support@lifecanvastech.com.