Virus Mapping | Screen and validate AAVs for desirable properties

Use Case: Screen viral vector tools for desirable properties

Confirm virus injections: Gauge different modes of virus delivery for efficient non-invasive gene transfer, unbiasedly and comprehensively throughout whole organs.

Confirm virus transductions: Assess natural and genetically-engineered virus capsids for their ability to transduce specific tissues, cell-types, and within discrete time-windows for delivery of various payloads.

Confirm virus gene expression: Evaluate different genetic control elements for their ability to promote or restrict gene expression to specific cell-types.

Mouse brain cleared and imaged for GFP expression after AAV injections and vector distribution after striatal injection. (A) Experimental setup, (B) coronal view, (C) top view, (D) lateral side view of injection side (E) Higher magnification of hippocampus 35 days, (F) overlay with viral vector DNA probe (red).

Figure 1a – 1f extracted from Nobre, R. J., Lobo, D. D., Henriques, C., Duarte, S. P., Lopes, S. M., Silva, A. C., Lopes, M. M., & Toonen, L. J. A. (2021). miRNA-mediated knockdown of ATXN3 alleviates molecular disease hallmarks in a mouse model for spinocerebellar ataxia type 3. Nucleic Acid Therapeutics, 31(5), 352-364. https://doi.org/10.1089/nat.2021.0020. Article distributed under the terms of the Creative Commons CC BY license.

For additional publications supporting this use case refer to:

Zheng et al. Cell 187(13):3236-3248 (2024) – See Figure 2f

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