(a) Diagram of the viral strategy for tracing the control network for insula→BNST cells. (b) Schematic of rabies tracing strategy. (c) Maximum projection image of 3D reconstruction of light sheet data set to illustrate insula→BNST control network. (d) Resampled images in the coronal plane at the level of the anterior insula. (e) At the level of the BNST. (f) At the level of thalamic and amygdalar structures. (g) Distribution of labeled neurons represented as a composite whole-brain density heatmap of semi-autonomously quantified insula→BNST control network cells as density in mm3. (h) Graphical representation of cell densities in top eight regions (by raw cell count) labeled as part of the labeled insula→BNST control network of five mice. (i) Top eight regions (by cell count per region/total cells in each respective brain × 100) of the labeled insula→BNST control network. (j) Top eight regions (by cell density) of the labeled insula→BNST control network.

Figure 6, extracted from: Luchsinger, J.R., Fetterly, T.L., Williford, K.M. et al. Delineation of an insula-BNST circuit engaged by struggling behavior that regulates avoidance in mice. Nat Commun 12, 3561 (2021). https://doi.org/10.1038/s41467-021-23674-z. Article is licensed under the Creative Commons Attribution 4.0 International License.