Whole Organ Mapping

Gain valuable new insights into your data with advanced tissue clearing, imaging, and analysis platforms.

LifeCanvas tissue processing pipeline

Intact tissue clearing combined with whole sample imaging and analysis can provide previously inaccessible levels of biological detail and understanding. LifeCanvas offers extensive expertise and state-of-the-art tools and technologies to enable access to these insights:

  • SmartClear II Pro – Tissue clearing instrument capable of processing up to four adult mouse brains or eight hemispheres simultaneously, fully clearing in 3-5 days.
  • SmartLabel – Advanced immunostaining device, capable of uniformly labeling fully intact, several millimeter-thick tissues with multiple probes in under 24 hours.
  • SmartSPIM – Light sheet microscope optimized for resolution, speed, and flexibility for imaging large samples. High resolution, single channel imaging of an intact adult mouse brain can be achieved in 60 minutes or less.
  • SmartAnalytics – Full service options including mouse brain atlas alignment, regional segmentation, fluorescence intensity measurements, and cell counting.

    Ready to start the conversation? Fill out our Service Request Form to get a quote for our tissue processing and image analysis services!

Featured Applications

LifeCanvas has perfected technology to label and image c-FOS+ cells throughout the CNS. We also offer robust models for detecting c-FOS+ cells in murine brain samples and providing customers with cell counts, densities, and heat maps quantifying levels of regional neural activity registered to the Allen brain atlas. If you are interested in running a pilot trial or a comprehensive cohort-level study, please reach out to us with the details of your project.

Researchers have developed transgenic mouse models of Alzheimer’s and Parkinson’s diseases that provide the neuroscience community with important tools to study disease progression and explore potential therapies. LifeCanvas offers researchers the opportunity to process and analyze samples for β-amyloid deposition and for the accumulation of α-synuclein in Lewy Bodies. Images are registered to the Allen brain atlas and levels of protein aggregation are determined for regionally specific brain areas.

LifeCanvas’ contract research services give researchers the opportunity to have brain samples cleared and imaged for endogenous fluorescent markers tagging select cells and processes.  For transgenic mice with endogenous reporters or for samples labeled via viral transfection, we can offer detection and analyses of labeled cell counts, densities, and heat maps registered to the Allen brain atlas. 

For custom projects, our sample immunolabeling technology is versatile and can be applied to common neuroscience biomarkers such as PV, NeuN, GFAP, IBA1, TH, ChAT, MBP, as well as nuclear and vascular stains. We also have extensive experience with other mammalian vital organs (e.g. spinal cord, lungs, pancreas, kidneys, intestines) and other types of samples including organoids and tumors.