Immunohistochemistry (IHC) and 3D histology are different approaches to detecting specific proteins in biological tissue.
Delipidation and refractive index (RI) -matching allow light to pass unhindered through the sample during microscopy. In effect, tissue clearing refers to the combined process of delipidation and optical clearing via index matching.
Delipidated tissues will appear translucent, while cleared and RI-matched tissues will appear transparent. Our preformulated EasyIndex solution prepares samples for sharp, informative volumetric imaging with light sheet microscopes like SmartSPIM and MegaSPIM.
The 500-mL EasyIndex bottle can be used for up to 12 whole mouse brains or similarly sized intact samples, or ~200 1 mm-thick sections. We provide RI = 1.52 standard solutions with RI = 1.46 solution available on request.
EasyIndex is ideal for index-matching multiple tissue types, immunolabeled tissues, and specimens cleared with aqueous methods like SHIELD or CLARITY.
Dong Yang, Yu Wang, Tianbo Qi, Xi Zhang, Leyao Shen, Jingrui Ma, Zhengyuan Pang, Neeraj K. Lal, Daniel B. McClatchy, Saba Heydari Seradj, Verina H. Leung, Kristina Wang, Yi Xie, Filip S. Polli, Anton Maximov, Oscar Christian Gonzalez, Luis de Lecea, Hollis T. Cline, Vineet Augustine, John R. Yates III, and Li Ye.
Rhiana C. Simon, Weston T. Fleming, Pranav Senthilkumar, Brandy A. Briones1,2, Kentaro K. Ishii, Madelyn M. Hjort, Madison M. Martin, Koichi Hashikawa, Andrea D. Sanders, Sam A. Golden, Garret D. Stuber.
Matthew R. Steinhart, Wouter H. van der Valk, Daniel Osorio, Sara A. Serdy, Jingyuan Zhang, Carl Nist-Lund, Jin Kim, Cynthia Moncada-Reid, Liang Sun, Jiyoon Lee, Karl R. Koehler
Immunohistochemistry (IHC) and 3D histology are different approaches to detecting specific proteins in biological tissue.
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