LifeCanvas Technologies is pioneering holistic intact-sample tissue processing with its sample-to-dataset pipeline and tools, seamlessly integrating each step in the workflow. As the only company to own the entire workflow, from tissue preservation, active clearing, antibody labeling, and refractive index-matching, to light-sheet imaging and image analysis, we are positioned to break down barriers and empower researchers with our streamlined approaches to ensure the best results and data possible.
Below you will find materials to help you make best use of LifeCanvas’s products, including the SHIELD tissue preservation protocol, tissue clearing resources, safety data sheets for reagents, and video tutorials.
Delipidating tissue to enable intact-sample labeling and imaging involves exposing samples to harsh treatments like high temperatures and pH changes. If left unchecked, this can cause damage to proteins and overall tissue structure, leading to incomplete or inaccurate image datasets. Therefore, to preserve the sample’s endogenous fluorescence and antigenicity along with tissue architecture, we use a novel tissue preservation technique called SHIELD that forms intramolecular bonds using polyfunctional, flexible epoxides to stabilize tissue architecture.
After SHIELD preservation, the tissue can be delipidated and either refractive index-matched for imaging or subjected to active immunohistochemical labeling. Notably, SHIELD-preserved tissues are well-suited for antibody multiplexing, i.e., iterative staining and de-staining, without loss of tissue antigenicity, to build up a rich picture of protein expression over repeated rounds of imaging. Check out the FAQ page for more information.
Full Pipeline Protocol (see “SHIELD” section beginning on page 3)
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